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[Study on the changing regularity of special antibody and expression of stomach and enteric involvement on SARS-coronavirus infection in the recovery period of severe acute respiratory syndrome].
Zhonghua Liu Xing Bing Xue Za Zhi. 2010 Jul; 31(7):795-9.ZL

Abstract

OBJECTIVE

To study the change of special antibodies titer IgG, IgM and nucleocapsid to SARS coronavirus (CoV) and observing the expression of stomach and enteric involvement on SARS-CoV infection by monoclonal antibody against N protein of SARS-CoV in the 7-year recovery period among family clustering cases of severe acute respiratory syndrome.

METHODS

Special antibody titer to SARS-CoV of 14 patients from 5 different families and their 10 kinfolks continuously tested by IFA and antigen-capturing ELISA methods. Samples were taken in the 1(st) - 7(th) year periods after SARS patients infected by SARS-CoV, being diluted and measured on it titers of three kinds of antibodies. Immunochemical staining with monoclonal antibody (mAb) against N protein of SARS-CoV was used to determine the stomach and enteric tissues among 5 SARS patients with their nucleocapsid antibody titer ascended obviously after 1(st)-7(th) year.

RESULTS

When testing the IgG antibody titer of the 14 SARS patients by IFA method, the average titer was 1/71 (95%CI: 1/58 - 1/85) in the 1(st) year, but began to descend in the following years, and the IgG antibody of the most SARS patients disappeared in the 7(th) year. Regarding the IgM titer, it disappeared in most of the SARS patients 1 year later. The average value of nucleocapsid antibody titer was 1/146 (95%CI: 1/122 - 1/171) in the 1(st) year, and it descended as the IgG antibody titer did. In 5 cases, differences appeared. The nucleocapsid antibody titer was between 1/156 and 1/210 in 3 cases, and 2 cases were normal. Immunochemical staining with mAb against N protein of SARS-CoV was identified in the stomach and enteric tissues of 5 SARS patients with the nucleocapsid antibody titer increased significantly, 1(st)-7(th) year later. The five patients were detected by gastroscopy detection and cell immunohistochemistry test. 3 cases showed N protein antibody positive in the serum, and positive immunohistochemical expression in most of the cytoplasm in the gastric tissue mucous gland epithelial cells. 1 case also expressed in the intestinal tissue slurry columnar epithelium and interstitial cells. The other two cases showed negative on both serum N protein antibody and immunohistochemical expression. The biopsy results of the 5 patients were as follows: 1 case diagnosed as "signet-ring cell carcinoma of the stomach and rectum multiple transfer", 1 case of gastric polyp, 1 case of superficial antral gastritis and 2 cases were normal.

CONCLUSION

By testing the special IgG, IgM, nucleocapsid antibody to SARS-CoV of the 14 family clustering cases, we found that they all decreased in the 7(th) year, and most of them disappeared. The nucleocapsid antibody titer was related to pathogenetic condition. SARS-CoV was proved to be still present in stomach and enteric tissues of SARS patients with the nucleocapsid antibody titer increased significantly after the 7(th) year.

Authors+Show Affiliations

Shi YL
Clinic Laboratory, General Hospital of Guangzhou Command, Guangzhou, China. shiyuling6@sina.com
Li LH
No affiliation info available
Sun ZH
No affiliation info available
Chen JY
No affiliation info available
Liao Y
No affiliation info available
Zeng LL
No affiliation info available
Zhang W
No affiliation info available
Chen XD
No affiliation info available
Cao C
No affiliation info available

MeSH

AdultAgedAntibodies, MonoclonalAntibodies, ViralFemaleFollow-Up StudiesGastrointestinal TractHumansImmunoglobulin GImmunoglobulin MMaleMiddle AgedNucleocapsid ProteinsSARS VirusSevere Acute Respiratory Syndrome

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21162846

Citation

Shi, Yu-ling, et al. "[Study On the Changing Regularity of Special Antibody and Expression of Stomach and Enteric Involvement On SARS-coronavirus Infection in the Recovery Period of Severe Acute Respiratory Syndrome]." Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, vol. 31, no. 7, 2010, pp. 795-9.
Shi YL, Li LH, Sun ZH, et al. [Study on the changing regularity of special antibody and expression of stomach and enteric involvement on SARS-coronavirus infection in the recovery period of severe acute respiratory syndrome]. Zhonghua Liu Xing Bing Xue Za Zhi. 2010;31(7):795-9.
Shi, Y. L., Li, L. H., Sun, Z. H., Chen, J. Y., Liao, Y., Zeng, L. L., Zhang, W., Chen, X. D., & Cao, C. (2010). [Study on the changing regularity of special antibody and expression of stomach and enteric involvement on SARS-coronavirus infection in the recovery period of severe acute respiratory syndrome]. Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, 31(7), 795-9.
Shi YL, et al. [Study On the Changing Regularity of Special Antibody and Expression of Stomach and Enteric Involvement On SARS-coronavirus Infection in the Recovery Period of Severe Acute Respiratory Syndrome]. Zhonghua Liu Xing Bing Xue Za Zhi. 2010;31(7):795-9. PubMed PMID: 21162846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Study on the changing regularity of special antibody and expression of stomach and enteric involvement on SARS-coronavirus infection in the recovery period of severe acute respiratory syndrome]. AU - Shi,Yu-ling, AU - Li,Lin-hai, AU - Sun,Zhao-hui, AU - Chen,Jian-yun, AU - Liao,Yang, AU - Zeng,Lan-lan, AU - Zhang,Wei, AU - Chen,Xiao-dong, AU - Cao,Cheng, PY - 2010/12/18/entrez PY - 2010/12/18/pubmed PY - 2013/6/21/medline SP - 795 EP - 9 JF - Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi JO - Zhonghua Liu Xing Bing Xue Za Zhi VL - 31 IS - 7 N2 - OBJECTIVE: To study the change of special antibodies titer IgG, IgM and nucleocapsid to SARS coronavirus (CoV) and observing the expression of stomach and enteric involvement on SARS-CoV infection by monoclonal antibody against N protein of SARS-CoV in the 7-year recovery period among family clustering cases of severe acute respiratory syndrome. METHODS: Special antibody titer to SARS-CoV of 14 patients from 5 different families and their 10 kinfolks continuously tested by IFA and antigen-capturing ELISA methods. Samples were taken in the 1(st) - 7(th) year periods after SARS patients infected by SARS-CoV, being diluted and measured on it titers of three kinds of antibodies. Immunochemical staining with monoclonal antibody (mAb) against N protein of SARS-CoV was used to determine the stomach and enteric tissues among 5 SARS patients with their nucleocapsid antibody titer ascended obviously after 1(st)-7(th) year. RESULTS: When testing the IgG antibody titer of the 14 SARS patients by IFA method, the average titer was 1/71 (95%CI: 1/58 - 1/85) in the 1(st) year, but began to descend in the following years, and the IgG antibody of the most SARS patients disappeared in the 7(th) year. Regarding the IgM titer, it disappeared in most of the SARS patients 1 year later. The average value of nucleocapsid antibody titer was 1/146 (95%CI: 1/122 - 1/171) in the 1(st) year, and it descended as the IgG antibody titer did. In 5 cases, differences appeared. The nucleocapsid antibody titer was between 1/156 and 1/210 in 3 cases, and 2 cases were normal. Immunochemical staining with mAb against N protein of SARS-CoV was identified in the stomach and enteric tissues of 5 SARS patients with the nucleocapsid antibody titer increased significantly, 1(st)-7(th) year later. The five patients were detected by gastroscopy detection and cell immunohistochemistry test. 3 cases showed N protein antibody positive in the serum, and positive immunohistochemical expression in most of the cytoplasm in the gastric tissue mucous gland epithelial cells. 1 case also expressed in the intestinal tissue slurry columnar epithelium and interstitial cells. The other two cases showed negative on both serum N protein antibody and immunohistochemical expression. The biopsy results of the 5 patients were as follows: 1 case diagnosed as "signet-ring cell carcinoma of the stomach and rectum multiple transfer", 1 case of gastric polyp, 1 case of superficial antral gastritis and 2 cases were normal. CONCLUSION: By testing the special IgG, IgM, nucleocapsid antibody to SARS-CoV of the 14 family clustering cases, we found that they all decreased in the 7(th) year, and most of them disappeared. The nucleocapsid antibody titer was related to pathogenetic condition. SARS-CoV was proved to be still present in stomach and enteric tissues of SARS patients with the nucleocapsid antibody titer increased significantly after the 7(th) year. SN - 0254-6450 UR - https://www.unboundmedicine.com/medline/citation/21162846/[Study_on_the_changing_regularity_of_special_antibody_and_expression_of_stomach_and_enteric_involvement_on_SARS_coronavirus_infection_in_the_recovery_period_of_severe_acute_respiratory_syndrome]_ DB - PRIME DP - Unbound Medicine ER -
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