Tags

Type your tag names separated by a space and hit enter

Chemical composition of bioactive pressurized extracts of Romanian aromatic plants.
J Chromatogr A. 2011 Jul 29; 1218(30):4918-27.JC

Abstract

In this contribution, pressurized liquid extraction (PLE) has been employed to isolate bioactive compounds from three native Romanian plants, oregano (Origanum vulgare), tarragon (Artemisia dracunculus) and wild thyme (Thymus serpyllum). Different PLE conditions have been tested including extraction with water, ethanol and their mixtures in a wide range of extraction temperatures (50-200°C), and the antioxidant capacity of the extracts was measured using different assays (DPPH radical scavenging, TEAC assay and Folin-Ciocalteau assay to measure total phenols). Moreover, a complete chemical characterization by using LC-MS/MS was carried out to be able to correlate the bioactivity with the particular chemical composition of each extract and plant. The use of PLE with water as a solvent at the highest temperature tested (200°C) always provided the highest extraction yields for the three studied plants, being maximum for oregano (>60%). Besides, oregano's pressurized water extracts at lower temperatures (50°C) presented the highest content on total phenols (184.9 mg gallic acid/g extract) and the best antioxidant activities (EC(50) 6.98 μg/ml). In general, oregano extracts were the most active, followed by wild thyme extracts. The antioxidant capacity measured by DPPH assay was highly correlated with the amount of total phenols. Moreover, the use of a LC-MS/MS method allowed the identification of 30 different phenolic compounds in the different extracts, including phenolic acids, flavones, flavanones and flavonols, which have an important influence on the total antioxidant capacity of the different extracts.

Links

Publisher Full Text

Authors+Show Affiliations

Miron TL
Department of Bioengineering, Faculty of Food Science and Engineering, Dunarea de Jos University, Galati, Romania.
Plaza M
No affiliation info available
Bahrim G
No affiliation info available
Ibáñez E
No affiliation info available
Herrero M
No affiliation info available

MeSH

Analysis of VarianceAntioxidantsArtemisiaBiphenyl CompoundsChemical FractionationChromatography, LiquidFlavonoidsHydroxybenzoatesOriganumPicratesPlant ExtractsTandem Mass SpectrometryThymus Plant

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21163488

Citation

Miron, T L., et al. "Chemical Composition of Bioactive Pressurized Extracts of Romanian Aromatic Plants." Journal of Chromatography. A, vol. 1218, no. 30, 2011, pp. 4918-27.
Miron TL, Plaza M, Bahrim G, et al. Chemical composition of bioactive pressurized extracts of Romanian aromatic plants. J Chromatogr A. 2011;1218(30):4918-27.
Miron, T. L., Plaza, M., Bahrim, G., Ibáñez, E., & Herrero, M. (2011). Chemical composition of bioactive pressurized extracts of Romanian aromatic plants. Journal of Chromatography. A, 1218(30), 4918-27. https://doi.org/10.1016/j.chroma.2010.11.055
Miron TL, et al. Chemical Composition of Bioactive Pressurized Extracts of Romanian Aromatic Plants. J Chromatogr A. 2011 Jul 29;1218(30):4918-27. PubMed PMID: 21163488.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical composition of bioactive pressurized extracts of Romanian aromatic plants. AU - Miron,T L, AU - Plaza,M, AU - Bahrim,G, AU - Ibáñez,E, AU - Herrero,M, Y1 - 2010/11/27/ PY - 2010/10/18/received PY - 2010/11/18/revised PY - 2010/11/19/accepted PY - 2010/12/18/entrez PY - 2010/12/18/pubmed PY - 2011/10/4/medline SP - 4918 EP - 27 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1218 IS - 30 N2 - In this contribution, pressurized liquid extraction (PLE) has been employed to isolate bioactive compounds from three native Romanian plants, oregano (Origanum vulgare), tarragon (Artemisia dracunculus) and wild thyme (Thymus serpyllum). Different PLE conditions have been tested including extraction with water, ethanol and their mixtures in a wide range of extraction temperatures (50-200°C), and the antioxidant capacity of the extracts was measured using different assays (DPPH radical scavenging, TEAC assay and Folin-Ciocalteau assay to measure total phenols). Moreover, a complete chemical characterization by using LC-MS/MS was carried out to be able to correlate the bioactivity with the particular chemical composition of each extract and plant. The use of PLE with water as a solvent at the highest temperature tested (200°C) always provided the highest extraction yields for the three studied plants, being maximum for oregano (>60%). Besides, oregano's pressurized water extracts at lower temperatures (50°C) presented the highest content on total phenols (184.9 mg gallic acid/g extract) and the best antioxidant activities (EC(50) 6.98 μg/ml). In general, oregano extracts were the most active, followed by wild thyme extracts. The antioxidant capacity measured by DPPH assay was highly correlated with the amount of total phenols. Moreover, the use of a LC-MS/MS method allowed the identification of 30 different phenolic compounds in the different extracts, including phenolic acids, flavones, flavanones and flavonols, which have an important influence on the total antioxidant capacity of the different extracts. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/21163488/Chemical_composition_of_bioactive_pressurized_extracts_of_Romanian_aromatic_plants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(10)01629-8 DB - PRIME DP - Unbound Medicine ER -