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High prevalence of plasmid-mediated quinolone resistance determinant aac(6')-Ib-cr amongst Salmonella enterica serotype Typhimurium isolates from hospitalised paediatric patients with diarrhoea in China.
Int J Antimicrob Agents. 2011 Feb; 37(2):152-5.IJ

Abstract

In this study, the antimicrobial susceptibilities and prevalence of plasmid-mediated quinolone resistance determinants amongst Salmonella enterica serotype Typhimurium isolates from hospitalised paediatric patients with diarrhoea in China were investigated. In total, 40 (64.5%) of 62 S. Typhimurium isolates were resistant to ciprofloxacin (minimum inhibitory concentration ≥0.5 μg/mL), comprising 28 isolates with low-level resistance and 12 isolates with high-level resistance. All ciprofloxacin-resistant isolates were multiresistant to other antimicrobial agents. Four pulsed-field gel electrophoresis (PFGE) clusters were found amongst the 40 ciprofloxacin-resistant isolates, amongst which PFGE clusters A, B, E and D accounted for 7, 4, 1 and 28 isolates, respectively. Two isolates with high-level ciprofloxacin resistance had two mutations in the quinolone resistance-determining regions (QRDRs) of gyrA and parC. The remaining ciprofloxacin-resistant isolates had only one mutation in the QRDR of gyrA. All 62 S. Typhimurium isolates were negative for qnr genes and qepA and 23 (37.1%) of the isolates were positive for aac(6')-Ib-cr. Nineteen isolates harbouring aac(6')-Ib-cr belonged to PFGE cluster D. A high prevalence of ciprofloxacin resistance and aac(6')-Ib-cr was found amongst S. Typhimurium isolates in China from hospitalised paediatric patients with diarrhoea not receiving quinolones. A single mutation in the QRDR of gyrA as well as production of AAC(6')-Ib-cr contributed to ciprofloxacin resistance. Clonal spread was responsible for the dissemination of aac(6')-Ib-cr amongst S. Typhimurium isolates.

Authors+Show Affiliations

Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21163630

Citation

Yu, Fangyou, et al. "High Prevalence of Plasmid-mediated Quinolone Resistance Determinant aac(6')-Ib-cr Amongst Salmonella Enterica Serotype Typhimurium Isolates From Hospitalised Paediatric Patients With Diarrhoea in China." International Journal of Antimicrobial Agents, vol. 37, no. 2, 2011, pp. 152-5.
Yu F, Chen Q, Yu X, et al. High prevalence of plasmid-mediated quinolone resistance determinant aac(6')-Ib-cr amongst Salmonella enterica serotype Typhimurium isolates from hospitalised paediatric patients with diarrhoea in China. Int J Antimicrob Agents. 2011;37(2):152-5.
Yu, F., Chen, Q., Yu, X., Pan, J., Li, Q., Yang, L., Chen, C., Zhuo, C., Li, X., Zhang, X., Huang, J., & Wang, L. (2011). High prevalence of plasmid-mediated quinolone resistance determinant aac(6')-Ib-cr amongst Salmonella enterica serotype Typhimurium isolates from hospitalised paediatric patients with diarrhoea in China. International Journal of Antimicrobial Agents, 37(2), 152-5. https://doi.org/10.1016/j.ijantimicag.2010.10.021
Yu F, et al. High Prevalence of Plasmid-mediated Quinolone Resistance Determinant aac(6')-Ib-cr Amongst Salmonella Enterica Serotype Typhimurium Isolates From Hospitalised Paediatric Patients With Diarrhoea in China. Int J Antimicrob Agents. 2011;37(2):152-5. PubMed PMID: 21163630.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High prevalence of plasmid-mediated quinolone resistance determinant aac(6')-Ib-cr amongst Salmonella enterica serotype Typhimurium isolates from hospitalised paediatric patients with diarrhoea in China. AU - Yu,Fangyou, AU - Chen,Qiang, AU - Yu,Xiaojun, AU - Pan,Jingye, AU - Li,Qiaoqiao, AU - Yang,Lehe, AU - Chen,Cong, AU - Zhuo,Chao, AU - Li,Xiaoqiang, AU - Zhang,Xueqing, AU - Huang,Jinwei, AU - Wang,Liangxing, Y1 - 2010/12/15/ PY - 2010/04/16/received PY - 2010/09/29/revised PY - 2010/10/18/accepted PY - 2010/12/18/entrez PY - 2010/12/18/pubmed PY - 2011/4/16/medline SP - 152 EP - 5 JF - International journal of antimicrobial agents JO - Int J Antimicrob Agents VL - 37 IS - 2 N2 - In this study, the antimicrobial susceptibilities and prevalence of plasmid-mediated quinolone resistance determinants amongst Salmonella enterica serotype Typhimurium isolates from hospitalised paediatric patients with diarrhoea in China were investigated. In total, 40 (64.5%) of 62 S. Typhimurium isolates were resistant to ciprofloxacin (minimum inhibitory concentration ≥0.5 μg/mL), comprising 28 isolates with low-level resistance and 12 isolates with high-level resistance. All ciprofloxacin-resistant isolates were multiresistant to other antimicrobial agents. Four pulsed-field gel electrophoresis (PFGE) clusters were found amongst the 40 ciprofloxacin-resistant isolates, amongst which PFGE clusters A, B, E and D accounted for 7, 4, 1 and 28 isolates, respectively. Two isolates with high-level ciprofloxacin resistance had two mutations in the quinolone resistance-determining regions (QRDRs) of gyrA and parC. The remaining ciprofloxacin-resistant isolates had only one mutation in the QRDR of gyrA. All 62 S. Typhimurium isolates were negative for qnr genes and qepA and 23 (37.1%) of the isolates were positive for aac(6')-Ib-cr. Nineteen isolates harbouring aac(6')-Ib-cr belonged to PFGE cluster D. A high prevalence of ciprofloxacin resistance and aac(6')-Ib-cr was found amongst S. Typhimurium isolates in China from hospitalised paediatric patients with diarrhoea not receiving quinolones. A single mutation in the QRDR of gyrA as well as production of AAC(6')-Ib-cr contributed to ciprofloxacin resistance. Clonal spread was responsible for the dissemination of aac(6')-Ib-cr amongst S. Typhimurium isolates. SN - 1872-7913 UR - https://www.unboundmedicine.com/medline/citation/21163630/High_prevalence_of_plasmid_mediated_quinolone_resistance_determinant_aac_6'__Ib_cr_amongst_Salmonella_enterica_serotype_Typhimurium_isolates_from_hospitalised_paediatric_patients_with_diarrhoea_in_China_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924-8579(10)00494-2 DB - PRIME DP - Unbound Medicine ER -