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Virulence profiling and quantification of verocytotoxin-producing Escherichia coli O145:H28 and O26:H11 isolated during an ice cream-related hemolytic uremic syndrome outbreak.
Foodborne Pathog Dis. 2011 Mar; 8(3):421-6.FP

Abstract

In September-October 2007, a mixed-serotype outbreak of verocytotoxin-producing Escherichia coli (VTEC) O145:H28 and O26:H11 occurred in the province of Antwerp, Belgium. Five girls aged between 2 and 11 years developed hemolytic uremic syndrome, and seven other coexposed persons with bloody diarrhea were identified. Laboratory confirmation of O145:H28 infection was obtained for three hemolytic uremic syndrome patients, one of whom was coinfected with O26:H11. The epidemiological and laboratory investigations revealed ice cream as the most likely source of the outbreak. The ice cream was produced at a local dairy farm using pasteurized milk. VTEC of both serotypes with indistinguishable pulsed-field gel electrophoresis patterns were isolated from patients, ice cream, and environmental samples. Quantitative analysis of the ice cream indicated concentrations of 2.4 and 0.03 CFU/g for VTEC O145 and O26, respectively. Virulence typing revealed that the repertoire of virulence genes carried by the O145:H28 outbreak strain was comparable to that of O157 VTEC and more exhaustive as compared to the O26:H11 outbreak strain and nonrelated clinical strains belonging to these serotypes. Taken together, these data suggest that O145:H28 played the most important role in this outbreak.

Authors+Show Affiliations

Belgian Reference Laboratory for VTEC/EHEC, Department of Microbiology and Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Brussels, Belgium. gbuvens@vub.ac.beNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21166561

Citation

Buvens, Glenn, et al. "Virulence Profiling and Quantification of Verocytotoxin-producing Escherichia Coli O145:H28 and O26:H11 Isolated During an Ice Cream-related Hemolytic Uremic Syndrome Outbreak." Foodborne Pathogens and Disease, vol. 8, no. 3, 2011, pp. 421-6.
Buvens G, Possé B, De Schrijver K, et al. Virulence profiling and quantification of verocytotoxin-producing Escherichia coli O145:H28 and O26:H11 isolated during an ice cream-related hemolytic uremic syndrome outbreak. Foodborne Pathog Dis. 2011;8(3):421-6.
Buvens, G., Possé, B., De Schrijver, K., De Zutter, L., Lauwers, S., & Piérard, D. (2011). Virulence profiling and quantification of verocytotoxin-producing Escherichia coli O145:H28 and O26:H11 isolated during an ice cream-related hemolytic uremic syndrome outbreak. Foodborne Pathogens and Disease, 8(3), 421-6. https://doi.org/10.1089/fpd.2010.0693
Buvens G, et al. Virulence Profiling and Quantification of Verocytotoxin-producing Escherichia Coli O145:H28 and O26:H11 Isolated During an Ice Cream-related Hemolytic Uremic Syndrome Outbreak. Foodborne Pathog Dis. 2011;8(3):421-6. PubMed PMID: 21166561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Virulence profiling and quantification of verocytotoxin-producing Escherichia coli O145:H28 and O26:H11 isolated during an ice cream-related hemolytic uremic syndrome outbreak. AU - Buvens,Glenn, AU - Possé,Björn, AU - De Schrijver,Koen, AU - De Zutter,Lieven, AU - Lauwers,Sabine, AU - Piérard,Denis, Y1 - 2010/12/18/ PY - 2010/12/21/entrez PY - 2010/12/21/pubmed PY - 2012/4/20/medline SP - 421 EP - 6 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 8 IS - 3 N2 - In September-October 2007, a mixed-serotype outbreak of verocytotoxin-producing Escherichia coli (VTEC) O145:H28 and O26:H11 occurred in the province of Antwerp, Belgium. Five girls aged between 2 and 11 years developed hemolytic uremic syndrome, and seven other coexposed persons with bloody diarrhea were identified. Laboratory confirmation of O145:H28 infection was obtained for three hemolytic uremic syndrome patients, one of whom was coinfected with O26:H11. The epidemiological and laboratory investigations revealed ice cream as the most likely source of the outbreak. The ice cream was produced at a local dairy farm using pasteurized milk. VTEC of both serotypes with indistinguishable pulsed-field gel electrophoresis patterns were isolated from patients, ice cream, and environmental samples. Quantitative analysis of the ice cream indicated concentrations of 2.4 and 0.03 CFU/g for VTEC O145 and O26, respectively. Virulence typing revealed that the repertoire of virulence genes carried by the O145:H28 outbreak strain was comparable to that of O157 VTEC and more exhaustive as compared to the O26:H11 outbreak strain and nonrelated clinical strains belonging to these serotypes. Taken together, these data suggest that O145:H28 played the most important role in this outbreak. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/21166561/Virulence_profiling_and_quantification_of_verocytotoxin_producing_Escherichia_coli_O145:H28_and_O26:H11_isolated_during_an_ice_cream_related_hemolytic_uremic_syndrome_outbreak_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2010.0693?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -