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Forkhead transcription factor Foxa1 is a novel target gene of C/EBPβ and suppresses the early phase of adipogenesis.
Gene. 2011 Mar 01; 473(2):150-6.GENE

Abstract

Forkhead/winged helix transcription factors (Foxs) regulate differentiation, metabolism, and development. Although Foxa1 is expressed in adipocytes, the roles and regulation of Foxa1 in them remain unclear. Here, we found that under the control of C/EBPβ, Foxa1 suppressed lipid accumulation and concomitantly caused a decrease in adipogenic gene expression in adipocytes. Foxa1 was expressed in undifferentiated mouse 3T3-L1 cells and in the early phase of adipogenesis, with its highest expression at 3h after the initiation of adipogenesis, which was followed by a subsequent decrease. SiRNA-mediated suppression of Foxa1 expression activated the expression of adipogenic genes such as PPARγ. Moreover, siRNAs for C/EBPβ, but not those for C/EBPδ, reduced Foxa1 mRNA and protein levels. The results of a promoter-reporter assay and chromatin immunoprecipitation assay demonstrated that C/EBPβ bound to the C/EBP binding element at -529 of the mouse Foxa1 promoter. Furthermore, siRNA-mediated knockdown of C/EBPβ decreased the promoter activity of mouse Foxa1 gene. These results suggest that Foxa1 plays a suppressive role in the early phase of adipogenesis, acting under the control of C/EBPβ, and might be involved in the regulation of the rate of progression of the early phase of adipogenesis.

Authors+Show Affiliations

Laboratory of Biodefense and Regulation, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka, Japan. fujimori@gly.oups.ac.jpNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21167261

Citation

Fujimori, Ko, and Fumio Amano. "Forkhead Transcription Factor Foxa1 Is a Novel Target Gene of C/EBPβ and Suppresses the Early Phase of Adipogenesis." Gene, vol. 473, no. 2, 2011, pp. 150-6.
Fujimori K, Amano F. Forkhead transcription factor Foxa1 is a novel target gene of C/EBPβ and suppresses the early phase of adipogenesis. Gene. 2011;473(2):150-6.
Fujimori, K., & Amano, F. (2011). Forkhead transcription factor Foxa1 is a novel target gene of C/EBPβ and suppresses the early phase of adipogenesis. Gene, 473(2), 150-6. https://doi.org/10.1016/j.gene.2010.12.002
Fujimori K, Amano F. Forkhead Transcription Factor Foxa1 Is a Novel Target Gene of C/EBPβ and Suppresses the Early Phase of Adipogenesis. Gene. 2011 Mar 1;473(2):150-6. PubMed PMID: 21167261.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Forkhead transcription factor Foxa1 is a novel target gene of C/EBPβ and suppresses the early phase of adipogenesis. AU - Fujimori,Ko, AU - Amano,Fumio, Y1 - 2010/12/15/ PY - 2010/09/08/received PY - 2010/11/22/revised PY - 2010/12/08/accepted PY - 2010/12/21/entrez PY - 2010/12/21/pubmed PY - 2011/4/7/medline SP - 150 EP - 6 JF - Gene JO - Gene VL - 473 IS - 2 N2 - Forkhead/winged helix transcription factors (Foxs) regulate differentiation, metabolism, and development. Although Foxa1 is expressed in adipocytes, the roles and regulation of Foxa1 in them remain unclear. Here, we found that under the control of C/EBPβ, Foxa1 suppressed lipid accumulation and concomitantly caused a decrease in adipogenic gene expression in adipocytes. Foxa1 was expressed in undifferentiated mouse 3T3-L1 cells and in the early phase of adipogenesis, with its highest expression at 3h after the initiation of adipogenesis, which was followed by a subsequent decrease. SiRNA-mediated suppression of Foxa1 expression activated the expression of adipogenic genes such as PPARγ. Moreover, siRNAs for C/EBPβ, but not those for C/EBPδ, reduced Foxa1 mRNA and protein levels. The results of a promoter-reporter assay and chromatin immunoprecipitation assay demonstrated that C/EBPβ bound to the C/EBP binding element at -529 of the mouse Foxa1 promoter. Furthermore, siRNA-mediated knockdown of C/EBPβ decreased the promoter activity of mouse Foxa1 gene. These results suggest that Foxa1 plays a suppressive role in the early phase of adipogenesis, acting under the control of C/EBPβ, and might be involved in the regulation of the rate of progression of the early phase of adipogenesis. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/21167261/Forkhead_transcription_factor_Foxa1_is_a_novel_target_gene_of_C/EBPβ_and_suppresses_the_early_phase_of_adipogenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(10)00457-9 DB - PRIME DP - Unbound Medicine ER -