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Molecular mapping of the new blast resistance genes Pi47 and Pi48 in the durably resistant local rice cultivar Xiangzi 3150.
Phytopathology. 2011 May; 101(5):620-6.P

Abstract

The indica rice cultivar Xiangzi 3150 (XZ3150) confers a high level of resistance to 95% of the isolates of Magnaporthe oryzae (the agent of rice blast disease) collected in Hunan Province, China. To identify the resistance (R) gene(s) controlling the high level of resistance in this cultivar, we developed 286 F(9) recombinant inbred lines (RILs) from a cross between XZ3150 and the highly susceptible cultivar CO39. Inoculation of the RILs and an F(2) population from a cross between the two cultivars with the avirulent isolate 193-1-1 in the growth chamber indicated the presence of two dominant R genes in XZ3150. A linkage map with 134 polymorphic simple sequence repeat and single feature polymorphism markers was constructed with the genotype data of the 286 RILs. Composite interval mapping (CIM) using the results of 193-1-1 inoculation showed that two major R genes, designated Pi47 and Pi48, were located between RM206 and RM224 on chromosome 11, and between RM5364 and RM7102 on chromosome 12, respectively. Interestingly, the CIM analysis of the four resistant components of the RILs to the field blast population revealed that Pi47 and Pi48 were also the major genetic factors responsible for the field resistance in XZ3150. The DNA markers linked to the new R genes identified in this study should be useful for further fine mapping, gene cloning, and marker-aided breeding of blast-resistant rice cultivars.

Authors+Show Affiliations

Hunan Agricultural University, Changsha, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21171885

Citation

Huang, Hongmei, et al. "Molecular Mapping of the New Blast Resistance Genes Pi47 and Pi48 in the Durably Resistant Local Rice Cultivar Xiangzi 3150." Phytopathology, vol. 101, no. 5, 2011, pp. 620-6.
Huang H, Huang L, Feng G, et al. Molecular mapping of the new blast resistance genes Pi47 and Pi48 in the durably resistant local rice cultivar Xiangzi 3150. Phytopathology. 2011;101(5):620-6.
Huang, H., Huang, L., Feng, G., Wang, S., Wang, Y., Liu, J., Jiang, N., Yan, W., Xu, L., Sun, P., Li, Z., Pan, S., Liu, X., Xiao, Y., Liu, E., Dai, L., & Wang, G. L. (2011). Molecular mapping of the new blast resistance genes Pi47 and Pi48 in the durably resistant local rice cultivar Xiangzi 3150. Phytopathology, 101(5), 620-6. https://doi.org/10.1094/PHYTO-08-10-0209
Huang H, et al. Molecular Mapping of the New Blast Resistance Genes Pi47 and Pi48 in the Durably Resistant Local Rice Cultivar Xiangzi 3150. Phytopathology. 2011;101(5):620-6. PubMed PMID: 21171885.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular mapping of the new blast resistance genes Pi47 and Pi48 in the durably resistant local rice cultivar Xiangzi 3150. AU - Huang,Hongmei, AU - Huang,Ling, AU - Feng,Guangping, AU - Wang,Suhua, AU - Wang,Yue, AU - Liu,Jinling, AU - Jiang,Nan, AU - Yan,Weiting, AU - Xu,Lingchao, AU - Sun,Pingyong, AU - Li,Zhiqiang, AU - Pan,Sujun, AU - Liu,Xionglun, AU - Xiao,Yinghui, AU - Liu,Erming, AU - Dai,Liangying, AU - Wang,Guo-Liang, PY - 2010/12/22/entrez PY - 2010/12/22/pubmed PY - 2012/1/18/medline SP - 620 EP - 6 JF - Phytopathology JO - Phytopathology VL - 101 IS - 5 N2 - The indica rice cultivar Xiangzi 3150 (XZ3150) confers a high level of resistance to 95% of the isolates of Magnaporthe oryzae (the agent of rice blast disease) collected in Hunan Province, China. To identify the resistance (R) gene(s) controlling the high level of resistance in this cultivar, we developed 286 F(9) recombinant inbred lines (RILs) from a cross between XZ3150 and the highly susceptible cultivar CO39. Inoculation of the RILs and an F(2) population from a cross between the two cultivars with the avirulent isolate 193-1-1 in the growth chamber indicated the presence of two dominant R genes in XZ3150. A linkage map with 134 polymorphic simple sequence repeat and single feature polymorphism markers was constructed with the genotype data of the 286 RILs. Composite interval mapping (CIM) using the results of 193-1-1 inoculation showed that two major R genes, designated Pi47 and Pi48, were located between RM206 and RM224 on chromosome 11, and between RM5364 and RM7102 on chromosome 12, respectively. Interestingly, the CIM analysis of the four resistant components of the RILs to the field blast population revealed that Pi47 and Pi48 were also the major genetic factors responsible for the field resistance in XZ3150. The DNA markers linked to the new R genes identified in this study should be useful for further fine mapping, gene cloning, and marker-aided breeding of blast-resistant rice cultivars. SN - 0031-949X UR - https://www.unboundmedicine.com/medline/citation/21171885/Molecular_mapping_of_the_new_blast_resistance_genes_Pi47_and_Pi48_in_the_durably_resistant_local_rice_cultivar_Xiangzi_3150_ L2 - https://apsjournals.apsnet.org/doi/10.1094/PHYTO-08-10-0209?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -