Tags

Type your tag names separated by a space and hit enter

The neuroprotective effect of modified Yeoldahanso-tang via autophagy enhancement in models of Parkinson's disease.
J Ethnopharmacol. 2011 Mar 24; 134(2):313-22.JE

Abstract

AIM OF STUDY

Modified Yeoldahanso-tang (MYH) is a Korean herbal formula, containing 10 herbs: Pueraria lobata (Willd.) Ohwi, Angelica tenuissima Nakai, Scutellaria baicalensis Georgi, Platycodon grandiflorum (Jacq), Angelicae Dahurica, Cimicifuga heracleifolia Kom, Raphanus sativa L., Polygala tenuifolia (Willd.), Acorus gramineus Soland. and Dimocarpus longan Lour. The constitutive ratio of the ten herbs is at 6:4:2:1:2:2:2:4:6:6 in dry weight. MYH has been used to treat amnesia, hypochondria and dementia in Korea. In this study, we explored the possibility of using MYH in the prevention and treatment of Parkinson's disease (PD). Specifically, we made an effort to demonstrate the neuroprotective effects of MYH using experimental methods similar to those used in a recent study of PD.

MATERIALS AND METHODS

1-Methyl-4-phenylpyridinium (MPP+) (400μM) was used to induce cytotoxicity in NGF (nerve growth factor)-differentiated PC12 cells. Cell viability was measured using a MTT assay. Induction of autophagy by MYH in NGF-differentiated PC12 cells was measured using an immunoblotting assay with LC3 and beclin 1 antibodies. The proteasomal inhibitor lactacystin (10μM) was used to cause UPS dysfunction in NGF-differentiated PC12 cells. Clearance of aggregated proteins by MYH was measured using an immunoblotting assay with an ubiquitin antibody. 1-Methyl-4-phenyl-1,2,3,6-tetrahydrophenylpyridine (MPTP) (20mg/kg, 4 times i.p.) caused substantia nigra injuries in C57BL/6 mice. Dopamine (DA) neurons were identified using a tyrosine hydroxylase-immunohistochemistry (TH-IHC) assay with a rabbit anti-TH antibody.

RESULTS

Our findings indicate that MYH provides protection against MPP+-induced injury in NGF-differentiated PC12 cell. And MYH provides neuroprotection against lactacystin-induced NGF-differentiated PC12 cell death, which effect is partially mediated by autophagy enhancement through enhanced degradation of aggregated proteins. Additionally, in a C57BL/6 mice model with MPTP-induced substantia nigra injuries, MYH inhibits both the loss of TH-positive neurons in the substantia nigra pars compacta (SNpc) and the reduction of the optical density of TH-IR fibers in the striatum (ST).

CONCLUSIONS

All of our results indicate that MYH treatment has neuroprotective effects that are partially mediated by autophagy enhancement. MYH may be a promising herbal formula for the prevention and treatment of neurodegenerative diseases, especially PD.

Authors+Show Affiliations

Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, 290 Daejeon-dong, Gangneung, Gangwon-do 210-340, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21172413

Citation

Bae, Nayoung, et al. "The Neuroprotective Effect of Modified Yeoldahanso-tang Via Autophagy Enhancement in Models of Parkinson's Disease." Journal of Ethnopharmacology, vol. 134, no. 2, 2011, pp. 313-22.
Bae N, Ahn T, Chung S, et al. The neuroprotective effect of modified Yeoldahanso-tang via autophagy enhancement in models of Parkinson's disease. J Ethnopharmacol. 2011;134(2):313-22.
Bae, N., Ahn, T., Chung, S., Oh, M. S., Ko, H., Oh, H., Park, G., & Yang, H. O. (2011). The neuroprotective effect of modified Yeoldahanso-tang via autophagy enhancement in models of Parkinson's disease. Journal of Ethnopharmacology, 134(2), 313-22. https://doi.org/10.1016/j.jep.2010.12.016
Bae N, et al. The Neuroprotective Effect of Modified Yeoldahanso-tang Via Autophagy Enhancement in Models of Parkinson's Disease. J Ethnopharmacol. 2011 Mar 24;134(2):313-22. PubMed PMID: 21172413.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The neuroprotective effect of modified Yeoldahanso-tang via autophagy enhancement in models of Parkinson's disease. AU - Bae,Nayoung, AU - Ahn,Taekwon, AU - Chung,Sungkwon, AU - Oh,Myung Sook, AU - Ko,Hyeonseok, AU - Oh,Hyungun, AU - Park,Gunhyuk, AU - Yang,Hyun Ok, Y1 - 2010/12/21/ PY - 2010/06/20/received PY - 2010/11/26/revised PY - 2010/12/10/accepted PY - 2010/12/22/entrez PY - 2010/12/22/pubmed PY - 2011/11/8/medline SP - 313 EP - 22 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 134 IS - 2 N2 - AIM OF STUDY: Modified Yeoldahanso-tang (MYH) is a Korean herbal formula, containing 10 herbs: Pueraria lobata (Willd.) Ohwi, Angelica tenuissima Nakai, Scutellaria baicalensis Georgi, Platycodon grandiflorum (Jacq), Angelicae Dahurica, Cimicifuga heracleifolia Kom, Raphanus sativa L., Polygala tenuifolia (Willd.), Acorus gramineus Soland. and Dimocarpus longan Lour. The constitutive ratio of the ten herbs is at 6:4:2:1:2:2:2:4:6:6 in dry weight. MYH has been used to treat amnesia, hypochondria and dementia in Korea. In this study, we explored the possibility of using MYH in the prevention and treatment of Parkinson's disease (PD). Specifically, we made an effort to demonstrate the neuroprotective effects of MYH using experimental methods similar to those used in a recent study of PD. MATERIALS AND METHODS: 1-Methyl-4-phenylpyridinium (MPP+) (400μM) was used to induce cytotoxicity in NGF (nerve growth factor)-differentiated PC12 cells. Cell viability was measured using a MTT assay. Induction of autophagy by MYH in NGF-differentiated PC12 cells was measured using an immunoblotting assay with LC3 and beclin 1 antibodies. The proteasomal inhibitor lactacystin (10μM) was used to cause UPS dysfunction in NGF-differentiated PC12 cells. Clearance of aggregated proteins by MYH was measured using an immunoblotting assay with an ubiquitin antibody. 1-Methyl-4-phenyl-1,2,3,6-tetrahydrophenylpyridine (MPTP) (20mg/kg, 4 times i.p.) caused substantia nigra injuries in C57BL/6 mice. Dopamine (DA) neurons were identified using a tyrosine hydroxylase-immunohistochemistry (TH-IHC) assay with a rabbit anti-TH antibody. RESULTS: Our findings indicate that MYH provides protection against MPP+-induced injury in NGF-differentiated PC12 cell. And MYH provides neuroprotection against lactacystin-induced NGF-differentiated PC12 cell death, which effect is partially mediated by autophagy enhancement through enhanced degradation of aggregated proteins. Additionally, in a C57BL/6 mice model with MPTP-induced substantia nigra injuries, MYH inhibits both the loss of TH-positive neurons in the substantia nigra pars compacta (SNpc) and the reduction of the optical density of TH-IR fibers in the striatum (ST). CONCLUSIONS: All of our results indicate that MYH treatment has neuroprotective effects that are partially mediated by autophagy enhancement. MYH may be a promising herbal formula for the prevention and treatment of neurodegenerative diseases, especially PD. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/21172413/The_neuroprotective_effect_of_modified_Yeoldahanso_tang_via_autophagy_enhancement_in_models_of_Parkinson's_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(10)00892-5 DB - PRIME DP - Unbound Medicine ER -