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[Phenotype change of airway smooth muscle cell in bronchial asthma rats and the intervention of Zhichuan capsule on it].
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010 Sep; 30(9):982-5.ZZ

Abstract

OBJECTIVE

To investigate the phenotype change of airway smooth muscle cell (SMC) in bronchial asthma and the effect of Zhichuan Capsule (ZCC) on it.

METHODS

Sixty male SD rats were randomized into 5 groups equally, the normal control group, the model group, and three treated groups treated with ZCC, Western medicine (WM) and ZCC + WM respectively. Excepting rats in the control group, they were all made into bronchial asthma model rats and received respective treatment. Histomorphology of the airway SMC was observed by electron microsope at the 4th week, and the expressions of smalpha-actin and sm-MHC were examined at the 2nd and the 4th week after provocation.

RESULTS

As compared with the control group, moyfilaments in SMC were lesser, secretive organelles were more and levels of smalpha-actin and sm-MHC expressions at the 4th week were lower in the model group (P < 0.05). While histomorphology of SMC in the WM group was as narmal, but some hypoxia changes were seen, such as the fracture of mitochondria cristae and deplation of matrix; histomorphology of SMC in the ZCC treated group and the ZCC + WM treated group were similar to that in the normal control group, and their expressions of smalpha-actin and sm-MHC were enhanced as compared to those in the model group (P < 0.05).

CONCLUSION

Airway SMC changes from contractile phenotype to synthetic phenotype in asthma rats, but the progress of change may be inhibited by ZCC.

Authors+Show Affiliations

Shanghai University of Traditional Chinese Medicine, Shanghai.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21179743

Citation

Liu, Fang, et al. "[Phenotype Change of Airway Smooth Muscle Cell in Bronchial Asthma Rats and the Intervention of Zhichuan Capsule On It]." Zhongguo Zhong Xi Yi Jie He Za Zhi Zhongguo Zhongxiyi Jiehe Zazhi = Chinese Journal of Integrated Traditional and Western Medicine, vol. 30, no. 9, 2010, pp. 982-5.
Liu F, Fang H, Tang BQ. [Phenotype change of airway smooth muscle cell in bronchial asthma rats and the intervention of Zhichuan capsule on it]. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010;30(9):982-5.
Liu, F., Fang, H., & Tang, B. Q. (2010). [Phenotype change of airway smooth muscle cell in bronchial asthma rats and the intervention of Zhichuan capsule on it]. Zhongguo Zhong Xi Yi Jie He Za Zhi Zhongguo Zhongxiyi Jiehe Zazhi = Chinese Journal of Integrated Traditional and Western Medicine, 30(9), 982-5.
Liu F, Fang H, Tang BQ. [Phenotype Change of Airway Smooth Muscle Cell in Bronchial Asthma Rats and the Intervention of Zhichuan Capsule On It]. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2010;30(9):982-5. PubMed PMID: 21179743.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Phenotype change of airway smooth muscle cell in bronchial asthma rats and the intervention of Zhichuan capsule on it]. AU - Liu,Fang, AU - Fang,Hong, AU - Tang,Bin-Qing, PY - 2010/12/25/entrez PY - 2010/12/25/pubmed PY - 2013/2/5/medline SP - 982 EP - 5 JF - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine JO - Zhongguo Zhong Xi Yi Jie He Za Zhi VL - 30 IS - 9 N2 - OBJECTIVE: To investigate the phenotype change of airway smooth muscle cell (SMC) in bronchial asthma and the effect of Zhichuan Capsule (ZCC) on it. METHODS: Sixty male SD rats were randomized into 5 groups equally, the normal control group, the model group, and three treated groups treated with ZCC, Western medicine (WM) and ZCC + WM respectively. Excepting rats in the control group, they were all made into bronchial asthma model rats and received respective treatment. Histomorphology of the airway SMC was observed by electron microsope at the 4th week, and the expressions of smalpha-actin and sm-MHC were examined at the 2nd and the 4th week after provocation. RESULTS: As compared with the control group, moyfilaments in SMC were lesser, secretive organelles were more and levels of smalpha-actin and sm-MHC expressions at the 4th week were lower in the model group (P < 0.05). While histomorphology of SMC in the WM group was as narmal, but some hypoxia changes were seen, such as the fracture of mitochondria cristae and deplation of matrix; histomorphology of SMC in the ZCC treated group and the ZCC + WM treated group were similar to that in the normal control group, and their expressions of smalpha-actin and sm-MHC were enhanced as compared to those in the model group (P < 0.05). CONCLUSION: Airway SMC changes from contractile phenotype to synthetic phenotype in asthma rats, but the progress of change may be inhibited by ZCC. SN - 1003-5370 UR - https://www.unboundmedicine.com/medline/citation/21179743/[Phenotype_change_of_airway_smooth_muscle_cell_in_bronchial_asthma_rats_and_the_intervention_of_Zhichuan_capsule_on_it]_ L2 - https://medlineplus.gov/herbalmedicine.html DB - PRIME DP - Unbound Medicine ER -