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Inhibitory activity of root canal irrigants against Candida albicans, Enterococcus faecalis and Staphylococcus aureus.
Braz Oral Res. 2010 Oct-Dec; 24(4):406-12.BO

Abstract

The present study evaluated the antimicrobial activity of three root canal irrigants against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus. These microorganisms were incubated in the presence of citric acid (6 and 10%), EDTA (17%), and NaOCl (0.5, 1.0, 2.5, and 5.25%). Agar diffusion tests were performed and redox indicator resazurin was used to evaluate the inhibitory effect of the irrigants on the metabolic activity of these microorganisms. The mean diameters of the inhibition zones for the C. albicans cultures were 11.6 mm (17% EDTA), 5.5 mm (0.5% NaOCl), 12.9 mm (1% NaOCl), 22.1 mm (2.5% NaOCl), and 28.5 mm (5.25% NaOCl). The mean diameters of the inhibition zones for E. faecalis were 2.8 mm (1% NaOCl), 5.4 mm (2.5% NaOCl), and 8.3 mm (5.25% NaOCl). For S. aureus, the mean values were 8.0 mm (17% EDTA), 3.0 mm (1% NaOCl), 8.8 mm (2.5% NaOCl), and 10.0 mm (5.25% NaOCl). Most of the irrigant solutions presented effective antimicrobial activity against C. albicans. A high inhibitory effect on the metabolic activity of E. faecalis was detected when the microorganisms were incubated with 17% EDTA. The same result was reached when S. aureus was incubated in the presence of > 2.5% NaOCl. Altogether, these results indicate that 2.5% and 5.25% NaOCl are microbicides against S. aureus while 0.5% and 1% NaOCl are only microbiostatic against the tested bacteria. The 6% and 10% citric acid as well as 17% EDTA did not affect the viability of any of the assayed microorganisms.

Authors+Show Affiliations

Federal University of Rio de Janeiro, RJ, Brazil. tatiana_kelly@yahoo.com.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21180960

Citation

Fidalgo, Tatiana Kelly da Silva, et al. "Inhibitory Activity of Root Canal Irrigants Against Candida Albicans, Enterococcus Faecalis and Staphylococcus Aureus." Brazilian Oral Research, vol. 24, no. 4, 2010, pp. 406-12.
Fidalgo TK, Barcelos R, Portela MB, et al. Inhibitory activity of root canal irrigants against Candida albicans, Enterococcus faecalis and Staphylococcus aureus. Braz Oral Res. 2010;24(4):406-12.
Fidalgo, T. K., Barcelos, R., Portela, M. B., Soares, R. M., Gleiser, R., & Silva-Filho, F. C. (2010). Inhibitory activity of root canal irrigants against Candida albicans, Enterococcus faecalis and Staphylococcus aureus. Brazilian Oral Research, 24(4), 406-12.
Fidalgo TK, et al. Inhibitory Activity of Root Canal Irrigants Against Candida Albicans, Enterococcus Faecalis and Staphylococcus Aureus. Braz Oral Res. 2010;24(4):406-12. PubMed PMID: 21180960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory activity of root canal irrigants against Candida albicans, Enterococcus faecalis and Staphylococcus aureus. AU - Fidalgo,Tatiana Kelly da Silva, AU - Barcelos,Roberta, AU - Portela,Maristela Barbosa, AU - Soares,Rosangela Maria de Araújo, AU - Gleiser,Rogério, AU - Silva-Filho,Fernando Costa e, PY - 2010/02/06/received PY - 2010/08/09/accepted PY - 2010/12/25/entrez PY - 2010/12/25/pubmed PY - 2011/3/23/medline SP - 406 EP - 12 JF - Brazilian oral research JO - Braz Oral Res VL - 24 IS - 4 N2 - The present study evaluated the antimicrobial activity of three root canal irrigants against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus. These microorganisms were incubated in the presence of citric acid (6 and 10%), EDTA (17%), and NaOCl (0.5, 1.0, 2.5, and 5.25%). Agar diffusion tests were performed and redox indicator resazurin was used to evaluate the inhibitory effect of the irrigants on the metabolic activity of these microorganisms. The mean diameters of the inhibition zones for the C. albicans cultures were 11.6 mm (17% EDTA), 5.5 mm (0.5% NaOCl), 12.9 mm (1% NaOCl), 22.1 mm (2.5% NaOCl), and 28.5 mm (5.25% NaOCl). The mean diameters of the inhibition zones for E. faecalis were 2.8 mm (1% NaOCl), 5.4 mm (2.5% NaOCl), and 8.3 mm (5.25% NaOCl). For S. aureus, the mean values were 8.0 mm (17% EDTA), 3.0 mm (1% NaOCl), 8.8 mm (2.5% NaOCl), and 10.0 mm (5.25% NaOCl). Most of the irrigant solutions presented effective antimicrobial activity against C. albicans. A high inhibitory effect on the metabolic activity of E. faecalis was detected when the microorganisms were incubated with 17% EDTA. The same result was reached when S. aureus was incubated in the presence of > 2.5% NaOCl. Altogether, these results indicate that 2.5% and 5.25% NaOCl are microbicides against S. aureus while 0.5% and 1% NaOCl are only microbiostatic against the tested bacteria. The 6% and 10% citric acid as well as 17% EDTA did not affect the viability of any of the assayed microorganisms. SN - 1807-3107 UR - https://www.unboundmedicine.com/medline/citation/21180960/Inhibitory_activity_of_root_canal_irrigants_against_Candida_albicans_Enterococcus_faecalis_and_Staphylococcus_aureus_ L2 - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242010000400006&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -