Tags

Type your tag names separated by a space and hit enter

Nociceptin/orphanin FQ receptor knockout rats: in vitro and in vivo studies.
Neuropharmacology. 2011 Mar; 60(4):572-9.N

Abstract

Nociceptin/orphanin FQ (N/OFQ) regulates several biological functions via selective activation of the N/OFQ peptide (NOP) receptor. Recently knockout rats for the NOP receptor gene (NOP(-/-)) have been generated; these animals were used in the present study to investigate their emotional (open field, elevated plus maze, and forced swimming test), locomotor (drag and rotarod test), and nociceptive (plantar and formalin test) phenotypes in comparison with their NOP(+/+) littermates. In addition, N/OFQ sensitivity has been assessed in electrically stimulated vas deferens tissues taken from NOP(+/+) and NOP(-/-) rats. In the elevated plus maze and forced swimming tests NOP(-/-) rats showed anxiety- and anti-depressant-like phenotype, respectively. No differences were found in the open field test. NOP(-/-) rats outperformed their NOP(+/+) littermates in two motor behaviour assays. Genetic ablation of the NOP receptor gene produced a statistically significant increase in nociceptive behaviour of the mutant rats in the formalin test. Finally, in the electrically stimulated rat vas deferens taken from NOP(+/+) tissues, N/OFQ inhibited in a concentration-dependent manner the electrically induced twitches while the peptide was inactive in tissues taken from NOP(-/-) animals. These results, in line with previous findings obtained with selective NOP receptor antagonists in mice and rats and with mouse knockout studies, clearly indicate that endogenous N/OFQ-NOP receptor signalling plays an important role in controlling anxiety- and mood-related behaviours, exercise-driven locomotor activity and nociception. These observations are relevant for defining the therapeutic indications (and contraindications) of NOP receptor antagonists.

Authors+Show Affiliations

Department of Experimental and Clinical Medicine, University of Ferrara, Ferrara, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21184763

Citation

Rizzi, Anna, et al. "Nociceptin/orphanin FQ Receptor Knockout Rats: in Vitro and in Vivo Studies." Neuropharmacology, vol. 60, no. 4, 2011, pp. 572-9.
Rizzi A, Molinari S, Marti M, et al. Nociceptin/orphanin FQ receptor knockout rats: in vitro and in vivo studies. Neuropharmacology. 2011;60(4):572-9.
Rizzi, A., Molinari, S., Marti, M., Marzola, G., & Calo', G. (2011). Nociceptin/orphanin FQ receptor knockout rats: in vitro and in vivo studies. Neuropharmacology, 60(4), 572-9. https://doi.org/10.1016/j.neuropharm.2010.12.010
Rizzi A, et al. Nociceptin/orphanin FQ Receptor Knockout Rats: in Vitro and in Vivo Studies. Neuropharmacology. 2011;60(4):572-9. PubMed PMID: 21184763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nociceptin/orphanin FQ receptor knockout rats: in vitro and in vivo studies. AU - Rizzi,Anna, AU - Molinari,Stefano, AU - Marti,Matteo, AU - Marzola,Giuliano, AU - Calo',Girolamo, Y1 - 2010/12/22/ PY - 2010/07/29/received PY - 2010/12/15/revised PY - 2010/12/15/accepted PY - 2010/12/28/entrez PY - 2010/12/28/pubmed PY - 2011/5/13/medline SP - 572 EP - 9 JF - Neuropharmacology JO - Neuropharmacology VL - 60 IS - 4 N2 - Nociceptin/orphanin FQ (N/OFQ) regulates several biological functions via selective activation of the N/OFQ peptide (NOP) receptor. Recently knockout rats for the NOP receptor gene (NOP(-/-)) have been generated; these animals were used in the present study to investigate their emotional (open field, elevated plus maze, and forced swimming test), locomotor (drag and rotarod test), and nociceptive (plantar and formalin test) phenotypes in comparison with their NOP(+/+) littermates. In addition, N/OFQ sensitivity has been assessed in electrically stimulated vas deferens tissues taken from NOP(+/+) and NOP(-/-) rats. In the elevated plus maze and forced swimming tests NOP(-/-) rats showed anxiety- and anti-depressant-like phenotype, respectively. No differences were found in the open field test. NOP(-/-) rats outperformed their NOP(+/+) littermates in two motor behaviour assays. Genetic ablation of the NOP receptor gene produced a statistically significant increase in nociceptive behaviour of the mutant rats in the formalin test. Finally, in the electrically stimulated rat vas deferens taken from NOP(+/+) tissues, N/OFQ inhibited in a concentration-dependent manner the electrically induced twitches while the peptide was inactive in tissues taken from NOP(-/-) animals. These results, in line with previous findings obtained with selective NOP receptor antagonists in mice and rats and with mouse knockout studies, clearly indicate that endogenous N/OFQ-NOP receptor signalling plays an important role in controlling anxiety- and mood-related behaviours, exercise-driven locomotor activity and nociception. These observations are relevant for defining the therapeutic indications (and contraindications) of NOP receptor antagonists. SN - 1873-7064 UR - https://www.unboundmedicine.com/medline/citation/21184763/Nociceptin/orphanin_FQ_receptor_knockout_rats:_in_vitro_and_in_vivo_studies_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0028-3908(10)00345-X DB - PRIME DP - Unbound Medicine ER -