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Multiple regulatory sites in the Bacillus subtilis citB promoter region.
J Bacteriol. 1990 Sep; 172(9):5408-15.JB

Abstract

The aconitase (citB) gene of Bacillus subtilis is repressed during growth in a medium that contains a rapidly metabolizable carbon source and a source of 2-ketoglutarate. It is derepressed when either of these nutrient sources becomes limiting. Repression by rapidly metabolizable carbon sources was shown previously to depend at least in part on a DNA sequence located 67 to 84 base pairs upstream of the start point of citB transcription. In the present work, this region and surrounding DNA were mutagenized to identify more precisely the target for carbon catabolite repression. Mutations in a symmetric sequence located between positions -73 and -59 led to constitutive transcription from the citB promoter in media that normally provoke catabolite repression. By gel mobility shift assays, it was shown that at least one protein in extracts of B. subtilis binds to the symmetric sequence and that DNA of constitutive mutants binds to this protein much less effectively. A second sequence located near position -45 was also implicated in this regulation. A second form of regulation of citB was also investigated. This gene is known to be derepressed when cells are induced to sporulate by exhaustion of a nutrient broth medium or limitation of guanine nucleotide synthesis. The mutations that led to constitutivity with respect to the carbon source had no effect on citB expression in nutrient broth medium, indicating that control by catabolite repression and control by components of nutrient broth (presumably amino acids) act by different mechanisms.

Links

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Authors+Show Affiliations

Fouet A
Department of Molecular Biology and Microbiology, Tufts University Health Sciences Campus, Boston, Massachusetts 02111.
Jin SF
No affiliation info available
Raffel G
No affiliation info available
Sonenshein AL
No affiliation info available

MeSH

Aconitate HydrataseAdenosineAnti-Bacterial AgentsBacillus subtilisBase SequenceEnzyme RepressionGenes, BacterialGenes, RegulatorGenotypeKineticsMolecular Sequence DataMutationPlasmidsPromoter Regions, GeneticSequence Homology, Nucleic Acidbeta-Galactosidase

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2118511

Citation

Fouet, A, et al. "Multiple Regulatory Sites in the Bacillus Subtilis citB Promoter Region." Journal of Bacteriology, vol. 172, no. 9, 1990, pp. 5408-15.
Fouet A, Jin SF, Raffel G, et al. Multiple regulatory sites in the Bacillus subtilis citB promoter region. J Bacteriol. 1990;172(9):5408-15.
Fouet, A., Jin, S. F., Raffel, G., & Sonenshein, A. L. (1990). Multiple regulatory sites in the Bacillus subtilis citB promoter region. Journal of Bacteriology, 172(9), 5408-15.
Fouet A, et al. Multiple Regulatory Sites in the Bacillus Subtilis citB Promoter Region. J Bacteriol. 1990;172(9):5408-15. PubMed PMID: 2118511.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiple regulatory sites in the Bacillus subtilis citB promoter region. AU - Fouet,A, AU - Jin,S F, AU - Raffel,G, AU - Sonenshein,A L, PY - 1990/9/1/pubmed PY - 1990/9/1/medline PY - 1990/9/1/entrez SP - 5408 EP - 15 JF - Journal of bacteriology JO - J Bacteriol VL - 172 IS - 9 N2 - The aconitase (citB) gene of Bacillus subtilis is repressed during growth in a medium that contains a rapidly metabolizable carbon source and a source of 2-ketoglutarate. It is derepressed when either of these nutrient sources becomes limiting. Repression by rapidly metabolizable carbon sources was shown previously to depend at least in part on a DNA sequence located 67 to 84 base pairs upstream of the start point of citB transcription. In the present work, this region and surrounding DNA were mutagenized to identify more precisely the target for carbon catabolite repression. Mutations in a symmetric sequence located between positions -73 and -59 led to constitutive transcription from the citB promoter in media that normally provoke catabolite repression. By gel mobility shift assays, it was shown that at least one protein in extracts of B. subtilis binds to the symmetric sequence and that DNA of constitutive mutants binds to this protein much less effectively. A second sequence located near position -45 was also implicated in this regulation. A second form of regulation of citB was also investigated. This gene is known to be derepressed when cells are induced to sporulate by exhaustion of a nutrient broth medium or limitation of guanine nucleotide synthesis. The mutations that led to constitutivity with respect to the carbon source had no effect on citB expression in nutrient broth medium, indicating that control by catabolite repression and control by components of nutrient broth (presumably amino acids) act by different mechanisms. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/2118511/Multiple_regulatory_sites_in_the_Bacillus_subtilis_citB_promoter_region_ DB - PRIME DP - Unbound Medicine ER -