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The synergistic effect of hierarchical assemblies of siRNA and chemotherapeutic drugs co-delivered into hepatic cancer cells.
Biomaterials 2011; 32(8):2222-32B

Abstract

Diblock copolymers (PEI-PCL) of poly(ε-caprolactone) (PCL) and linear poly(ethylene imine) (PEI) were synthesized and assembled to biodegradable nano-carriers for co-delivery of BCL-2 siRNA and doxorubicin (DOX). Folic acid as a tumor-targeting ligand was conjugated to the polyanion, poly(ethylene glycol)-block-poly(glutamic acid) (FA-PEG-PGA). Driven by the electrostatic interaction, FA-PEG-PGA was coated onto the surface of the cationic PEI-PCL nanoparticles pre-loaded with siRNA and DOX, potentiating a ligand-directed delivery to human hepatic cancer cells Bel-7402. At certain N/P and C/N ratios (N/P: PEI-PCL nitrogen to siRNA phosphate; C/N: FA-PEG-PGA carboxyl to PEI-PCL amine), the nanoparticles exhibited not only high transfection efficiency but also ideally controlled release of drug. Compared to non-specific delivery, the folate-targeted delivery of BCL-2 siRNA resulted in more significant gene suppression at both the BCL-2 mRNA and protein expression levels, inducing cancer cell apoptosis and improving the therapeutic efficacy of the co-administered DOX. Herein we demonstrated that co-loading siRNA and small molecular drug in a multifunctional hierarchical nano-assembly enabled simultaneously delivering siRNA and drug into the same cancer cells, yielding synergistic effect of RNA interference and chemotherapy in cancer.

Authors+Show Affiliations

Center of Biomedical Engineering, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510275, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21186059

Citation

Cao, Nuo, et al. "The Synergistic Effect of Hierarchical Assemblies of siRNA and Chemotherapeutic Drugs Co-delivered Into Hepatic Cancer Cells." Biomaterials, vol. 32, no. 8, 2011, pp. 2222-32.
Cao N, Cheng D, Zou S, et al. The synergistic effect of hierarchical assemblies of siRNA and chemotherapeutic drugs co-delivered into hepatic cancer cells. Biomaterials. 2011;32(8):2222-32.
Cao, N., Cheng, D., Zou, S., Ai, H., Gao, J., & Shuai, X. (2011). The synergistic effect of hierarchical assemblies of siRNA and chemotherapeutic drugs co-delivered into hepatic cancer cells. Biomaterials, 32(8), pp. 2222-32. doi:10.1016/j.biomaterials.2010.11.061.
Cao N, et al. The Synergistic Effect of Hierarchical Assemblies of siRNA and Chemotherapeutic Drugs Co-delivered Into Hepatic Cancer Cells. Biomaterials. 2011;32(8):2222-32. PubMed PMID: 21186059.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The synergistic effect of hierarchical assemblies of siRNA and chemotherapeutic drugs co-delivered into hepatic cancer cells. AU - Cao,Nuo, AU - Cheng,Du, AU - Zou,Seyin, AU - Ai,Hua, AU - Gao,Jinming, AU - Shuai,Xintao, Y1 - 2010/12/24/ PY - 2010/09/21/received PY - 2010/11/26/accepted PY - 2010/12/28/entrez PY - 2010/12/28/pubmed PY - 2011/4/27/medline SP - 2222 EP - 32 JF - Biomaterials JO - Biomaterials VL - 32 IS - 8 N2 - Diblock copolymers (PEI-PCL) of poly(ε-caprolactone) (PCL) and linear poly(ethylene imine) (PEI) were synthesized and assembled to biodegradable nano-carriers for co-delivery of BCL-2 siRNA and doxorubicin (DOX). Folic acid as a tumor-targeting ligand was conjugated to the polyanion, poly(ethylene glycol)-block-poly(glutamic acid) (FA-PEG-PGA). Driven by the electrostatic interaction, FA-PEG-PGA was coated onto the surface of the cationic PEI-PCL nanoparticles pre-loaded with siRNA and DOX, potentiating a ligand-directed delivery to human hepatic cancer cells Bel-7402. At certain N/P and C/N ratios (N/P: PEI-PCL nitrogen to siRNA phosphate; C/N: FA-PEG-PGA carboxyl to PEI-PCL amine), the nanoparticles exhibited not only high transfection efficiency but also ideally controlled release of drug. Compared to non-specific delivery, the folate-targeted delivery of BCL-2 siRNA resulted in more significant gene suppression at both the BCL-2 mRNA and protein expression levels, inducing cancer cell apoptosis and improving the therapeutic efficacy of the co-administered DOX. Herein we demonstrated that co-loading siRNA and small molecular drug in a multifunctional hierarchical nano-assembly enabled simultaneously delivering siRNA and drug into the same cancer cells, yielding synergistic effect of RNA interference and chemotherapy in cancer. SN - 1878-5905 UR - https://www.unboundmedicine.com/medline/citation/21186059/The_synergistic_effect_of_hierarchical_assemblies_of_siRNA_and_chemotherapeutic_drugs_co_delivered_into_hepatic_cancer_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0142-9612(10)01516-4 DB - PRIME DP - Unbound Medicine ER -