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[Effect of hyperbaric oxygenation treatment on the apoptotic cell death pathway after transient focal cerebral ischemia].

Abstract

AIM

To evaluate the effects of administration of hyperbaric oxygenation(HBO) when initiated at different time after acute transient ischemia. Apoptosis in the ischemic penumbra was further investigated to search for the possible mechanism.

METHODS

The male SD rats were randomly assigned to the following groups: control, HBO therapy initiated 3 h after ischemia, HBO therapy initiated 6 h after ischemia, HBO therapy initiated 12 h after ischemia. All animals were subjected to 90 min intraluminal middle cerebral artery occlusion (MCAO) with the regional cerebral blood flow monitored in vivo by laser Doppler flowmetry. HBO treatment was performed in a pressure chamber with 100% O2, 3 arm for 1 h. Neurological deficits and infarct volumes were assessed at 24 hours after ischemia. The immunohistochemical changes of apoptosis in the penumbra were evaluated by detecting the expression of cleaved Caspase-3, cleaved Caspase-9, Bcl-2, Bax and TUNEL staining.

RESULTS

HBO therapy initiated at 3 and 6 hours after ischemia significantly improved the neurological function and reduced infarct volume. Meanwhile, it increased the expression of Bcl-2 protein and decreased the expression of activated Caspase-3, activated Caspase-9 and TUNEL-positive cells. However, HBO therapy administrated 12 hours after ischemia aggravated the neurological deficits and enlarged infarct volume, while it showed no significant reduction of apoptotic change compared with control.

CONCLUSION

There is a therapeutic window for the use of HBO in acute transient cerebral ischemia in rats. HBO-treatment is highly effective in reducing infarct volume when initiated up to 6h after the onset of ischemia. Inhibition of apoptotic cell death in the penumbra appears to be the underlying protective effect of early therapy.

Authors+Show Affiliations

Department of Neurology, Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou 310009, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21186562

Citation

Lou, Min, et al. "[Effect of Hyperbaric Oxygenation Treatment On the Apoptotic Cell Death Pathway After Transient Focal Cerebral Ischemia]." Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology, vol. 22, no. 1, 2006, pp. 1-5.
Lou M, Ding MP, Wen SQ. [Effect of hyperbaric oxygenation treatment on the apoptotic cell death pathway after transient focal cerebral ischemia]. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2006;22(1):1-5.
Lou, M., Ding, M. P., & Wen, S. Q. (2006). [Effect of hyperbaric oxygenation treatment on the apoptotic cell death pathway after transient focal cerebral ischemia]. Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology, 22(1), 1-5.
Lou M, Ding MP, Wen SQ. [Effect of Hyperbaric Oxygenation Treatment On the Apoptotic Cell Death Pathway After Transient Focal Cerebral Ischemia]. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2006;22(1):1-5. PubMed PMID: 21186562.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of hyperbaric oxygenation treatment on the apoptotic cell death pathway after transient focal cerebral ischemia]. AU - Lou,Min, AU - Ding,Mei-Ping, AU - Wen,Shu-Qun, PY - 2010/12/29/entrez PY - 2006/2/1/pubmed PY - 2012/6/1/medline SP - 1 EP - 5 JF - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology JO - Zhongguo Ying Yong Sheng Li Xue Za Zhi VL - 22 IS - 1 N2 - AIM: To evaluate the effects of administration of hyperbaric oxygenation(HBO) when initiated at different time after acute transient ischemia. Apoptosis in the ischemic penumbra was further investigated to search for the possible mechanism. METHODS: The male SD rats were randomly assigned to the following groups: control, HBO therapy initiated 3 h after ischemia, HBO therapy initiated 6 h after ischemia, HBO therapy initiated 12 h after ischemia. All animals were subjected to 90 min intraluminal middle cerebral artery occlusion (MCAO) with the regional cerebral blood flow monitored in vivo by laser Doppler flowmetry. HBO treatment was performed in a pressure chamber with 100% O2, 3 arm for 1 h. Neurological deficits and infarct volumes were assessed at 24 hours after ischemia. The immunohistochemical changes of apoptosis in the penumbra were evaluated by detecting the expression of cleaved Caspase-3, cleaved Caspase-9, Bcl-2, Bax and TUNEL staining. RESULTS: HBO therapy initiated at 3 and 6 hours after ischemia significantly improved the neurological function and reduced infarct volume. Meanwhile, it increased the expression of Bcl-2 protein and decreased the expression of activated Caspase-3, activated Caspase-9 and TUNEL-positive cells. However, HBO therapy administrated 12 hours after ischemia aggravated the neurological deficits and enlarged infarct volume, while it showed no significant reduction of apoptotic change compared with control. CONCLUSION: There is a therapeutic window for the use of HBO in acute transient cerebral ischemia in rats. HBO-treatment is highly effective in reducing infarct volume when initiated up to 6h after the onset of ischemia. Inhibition of apoptotic cell death in the penumbra appears to be the underlying protective effect of early therapy. SN - 1000-6834 UR - https://www.unboundmedicine.com/medline/citation/21186562/[Effect_of_hyperbaric_oxygenation_treatment_on_the_apoptotic_cell_death_pathway_after_transient_focal_cerebral_ischemia]_ DB - PRIME DP - Unbound Medicine ER -