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Validity of quantitative unspun urine microscopy, dipstick test leucocyte esterase and nitrite tests in rapidly diagnosing urinary tract infections.
J Assoc Physicians India. 2010 Aug; 58:485-7.JA

Abstract

OBJECTIVES

Rapid diagnostic tests can screen out negative samples and can save valuable time and money. The study was conducted to assess the usefulness of leukocyte esterase, nitrate reductase and quantitative microscopic unspun urine wet mount examination in rapidly diagnosing urinary tract infections (UTI).

METHODS

Four hundred and fifty samples were tested by semi-quantitative culture on cysteine lactose electrolyte deficient medium, microscopic examination of unspun urine for significant pyuria, dipstick leucocyte esterase test (LET) and nitrite test (NT). Culture was used as gold standard to evaluate the performance of direct microscopy and dipstick tests.

RESULTS

Urine culture examination revealed significant bacteriuria (>10(5) cfu/ml) 98 (21.8%), in urine samples. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and the diagnostic odds ratio (DOR) of the dip-stick LET were 73.5%, 58.5%, 33.0%, 88.8%, and 3.9 respectively; those of the dip-stick NT were 57.1%, 78.7%, 42.7%, 86.8%, and 4.9 respectively; and those for microscopic significant pyuria detection were 68.4%, 60.8%, 32.7%, 87.3%, and 3.4 respectively. Highest sensitivity (95.9%), NPV (97.9%) and DOR (25.7) was obtained on combining microscopy and dip-stick LET and NT (either of them positive). This can potentially cut costs by 79%.

CONCLUSION

Quantitative unspun urine wet mount microscopy and dipstick tests for leucocyte esterase and nitrite test should be added into routine laboratory practices for faster diagnosis of UTI.

Authors+Show Affiliations

Department of Medical Microbiology, PGIMER, Chandigarh.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

21189695

Citation

Taneja, N, et al. "Validity of Quantitative Unspun Urine Microscopy, Dipstick Test Leucocyte Esterase and Nitrite Tests in Rapidly Diagnosing Urinary Tract Infections." The Journal of the Association of Physicians of India, vol. 58, 2010, pp. 485-7.
Taneja N, Chatterjee SS, Singh M, et al. Validity of quantitative unspun urine microscopy, dipstick test leucocyte esterase and nitrite tests in rapidly diagnosing urinary tract infections. J Assoc Physicians India. 2010;58:485-7.
Taneja, N., Chatterjee, S. S., Singh, M., Sivapriya, S., Sharma, M., & Sharma, S. K. (2010). Validity of quantitative unspun urine microscopy, dipstick test leucocyte esterase and nitrite tests in rapidly diagnosing urinary tract infections. The Journal of the Association of Physicians of India, 58, 485-7.
Taneja N, et al. Validity of Quantitative Unspun Urine Microscopy, Dipstick Test Leucocyte Esterase and Nitrite Tests in Rapidly Diagnosing Urinary Tract Infections. J Assoc Physicians India. 2010;58:485-7. PubMed PMID: 21189695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validity of quantitative unspun urine microscopy, dipstick test leucocyte esterase and nitrite tests in rapidly diagnosing urinary tract infections. AU - Taneja,N, AU - Chatterjee,S S, AU - Singh,M, AU - Sivapriya,S, AU - Sharma,M, AU - Sharma,S K, PY - 2010/12/31/entrez PY - 2010/12/31/pubmed PY - 2011/1/12/medline SP - 485 EP - 7 JF - The Journal of the Association of Physicians of India JO - J Assoc Physicians India VL - 58 N2 - OBJECTIVES: Rapid diagnostic tests can screen out negative samples and can save valuable time and money. The study was conducted to assess the usefulness of leukocyte esterase, nitrate reductase and quantitative microscopic unspun urine wet mount examination in rapidly diagnosing urinary tract infections (UTI). METHODS: Four hundred and fifty samples were tested by semi-quantitative culture on cysteine lactose electrolyte deficient medium, microscopic examination of unspun urine for significant pyuria, dipstick leucocyte esterase test (LET) and nitrite test (NT). Culture was used as gold standard to evaluate the performance of direct microscopy and dipstick tests. RESULTS: Urine culture examination revealed significant bacteriuria (>10(5) cfu/ml) 98 (21.8%), in urine samples. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and the diagnostic odds ratio (DOR) of the dip-stick LET were 73.5%, 58.5%, 33.0%, 88.8%, and 3.9 respectively; those of the dip-stick NT were 57.1%, 78.7%, 42.7%, 86.8%, and 4.9 respectively; and those for microscopic significant pyuria detection were 68.4%, 60.8%, 32.7%, 87.3%, and 3.4 respectively. Highest sensitivity (95.9%), NPV (97.9%) and DOR (25.7) was obtained on combining microscopy and dip-stick LET and NT (either of them positive). This can potentially cut costs by 79%. CONCLUSION: Quantitative unspun urine wet mount microscopy and dipstick tests for leucocyte esterase and nitrite test should be added into routine laboratory practices for faster diagnosis of UTI. SN - 0004-5772 UR - https://www.unboundmedicine.com/medline/citation/21189695/Validity_of_quantitative_unspun_urine_microscopy_dipstick_test_leucocyte_esterase_and_nitrite_tests_in_rapidly_diagnosing_urinary_tract_infections_ L2 - https://medlineplus.gov/urineandurination.html DB - PRIME DP - Unbound Medicine ER -