Tags

Type your tag names separated by a space and hit enter

Inhibition of NF-kappa B can enhance Fas-mediated apoptosis in leukemia cell line HL-60.
Front Med China. 2010 Sep; 4(3):323-8.FM

Abstract

This study explored the effects of nuclear factor-kappa B (NF-κB) inhibitor Bay 11-7082 on Fas/FasL system and Fas-mediated apoptosis in cell line HL-60 cells. The mRNA and protein levels of Fas, FasL, and X-linked inhibitor of apoptosis protein (XIAP) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry (FCM); the level of sFasL was evaluated by enzyme-linked immunosorbent assay (ELISA); and apoptosis was determined by FCM. After treatment with Bay 11-7082, the mRNA and protein levels of FasL and XIAP in HL-60 cells were significantly lower than in the controls (P<0.05), but the mRNA and protein levels of Fas and sFasL did not change significantly (P>0.05). Apoptotic rate of HL-60 cells treated with Bay 11-7082 was significantly higher than in the controls (P<0.05). Therefore, we conclude that Bay 11-7082 can enhance Fas-mediated apoptosis in HL-60 cells by downregulating FasL and XIAP levels.

Authors+Show Affiliations

Department of Hematology, The Central Hospital of Wuhan, and Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014, China. wlmarx96@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21191839

Citation

Wang, Li, et al. "Inhibition of NF-kappa B Can Enhance Fas-mediated Apoptosis in Leukemia Cell Line HL-60." Frontiers of Medicine in China, vol. 4, no. 3, 2010, pp. 323-8.
Wang L, Zhao S, Wang HX, et al. Inhibition of NF-kappa B can enhance Fas-mediated apoptosis in leukemia cell line HL-60. Front Med China. 2010;4(3):323-8.
Wang, L., Zhao, S., Wang, H. X., & Zou, P. (2010). Inhibition of NF-kappa B can enhance Fas-mediated apoptosis in leukemia cell line HL-60. Frontiers of Medicine in China, 4(3), 323-8. https://doi.org/10.1007/s11684-010-0026-5
Wang L, et al. Inhibition of NF-kappa B Can Enhance Fas-mediated Apoptosis in Leukemia Cell Line HL-60. Front Med China. 2010;4(3):323-8. PubMed PMID: 21191839.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of NF-kappa B can enhance Fas-mediated apoptosis in leukemia cell line HL-60. AU - Wang,Li, AU - Zhao,Shi, AU - Wang,Hong-Xiang, AU - Zou,Ping, Y1 - 2010/04/24/ PY - 2009/10/26/received PY - 2009/11/28/accepted PY - 2010/12/31/entrez PY - 2010/12/31/pubmed PY - 2011/5/14/medline SP - 323 EP - 8 JF - Frontiers of medicine in China JO - Front Med China VL - 4 IS - 3 N2 - This study explored the effects of nuclear factor-kappa B (NF-κB) inhibitor Bay 11-7082 on Fas/FasL system and Fas-mediated apoptosis in cell line HL-60 cells. The mRNA and protein levels of Fas, FasL, and X-linked inhibitor of apoptosis protein (XIAP) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry (FCM); the level of sFasL was evaluated by enzyme-linked immunosorbent assay (ELISA); and apoptosis was determined by FCM. After treatment with Bay 11-7082, the mRNA and protein levels of FasL and XIAP in HL-60 cells were significantly lower than in the controls (P<0.05), but the mRNA and protein levels of Fas and sFasL did not change significantly (P>0.05). Apoptotic rate of HL-60 cells treated with Bay 11-7082 was significantly higher than in the controls (P<0.05). Therefore, we conclude that Bay 11-7082 can enhance Fas-mediated apoptosis in HL-60 cells by downregulating FasL and XIAP levels. SN - 1673-7458 UR - https://www.unboundmedicine.com/medline/citation/21191839/Inhibition_of_NF-kappa_B_can_enhance_Fas-mediated_apoptosis_in_leukemia_cell_line_HL-60. L2 - https://antibodies.cancer.gov/detail/CPTC-FAS-1 DB - PRIME DP - Unbound Medicine ER -