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Autoimmune regulator regulates autophagy in THP-1 human monocytes.
Front Med China. 2010 Sep; 4(3):336-41.FM

Abstract

The autoimmune regulator (AIRE) is a crucial factor for the induction of central tolerance, and mutations in this gene lead to abnormal immune responses. However, the role of AIRE in autophagy in immune cells, especially in monocytes, is obscure. In the present study, we found that overexpression of AIRE in THP-1 human monocytes resulted in increased endogenous light chain 3 (LC3)-II level and elevated LC3 positive vesicles. Moreover, an autophagy inhibitor or knockdown of AIRE by small interference RNA attenuated these effects. In contrast, the expression of p62/SQSTM1 remained unchanged in THP-1 cells after the corresponding treatment. Our findings indicate that AIRE plays a role in the regulation of autophagy in THP-1 human monocytes.

Authors+Show Affiliations

Clinical Laboratory Medicine Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. sliang@smail.hust.edu.cnNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21191841

Citation

Shi, Liang, et al. "Autoimmune Regulator Regulates Autophagy in THP-1 Human Monocytes." Frontiers of Medicine in China, vol. 4, no. 3, 2010, pp. 336-41.
Shi L, Hu LH, Li YR. Autoimmune regulator regulates autophagy in THP-1 human monocytes. Front Med China. 2010;4(3):336-41.
Shi, L., Hu, L. H., & Li, Y. R. (2010). Autoimmune regulator regulates autophagy in THP-1 human monocytes. Frontiers of Medicine in China, 4(3), 336-41. https://doi.org/10.1007/s11684-010-0096-4
Shi L, Hu LH, Li YR. Autoimmune Regulator Regulates Autophagy in THP-1 Human Monocytes. Front Med China. 2010;4(3):336-41. PubMed PMID: 21191841.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Autoimmune regulator regulates autophagy in THP-1 human monocytes. AU - Shi,Liang, AU - Hu,Li-Hua, AU - Li,Yi-Rong, Y1 - 2010/08/05/ PY - 2010/04/22/received PY - 2010/06/10/accepted PY - 2010/12/31/entrez PY - 2010/12/31/pubmed PY - 2011/5/14/medline SP - 336 EP - 41 JF - Frontiers of medicine in China JO - Front Med China VL - 4 IS - 3 N2 - The autoimmune regulator (AIRE) is a crucial factor for the induction of central tolerance, and mutations in this gene lead to abnormal immune responses. However, the role of AIRE in autophagy in immune cells, especially in monocytes, is obscure. In the present study, we found that overexpression of AIRE in THP-1 human monocytes resulted in increased endogenous light chain 3 (LC3)-II level and elevated LC3 positive vesicles. Moreover, an autophagy inhibitor or knockdown of AIRE by small interference RNA attenuated these effects. In contrast, the expression of p62/SQSTM1 remained unchanged in THP-1 cells after the corresponding treatment. Our findings indicate that AIRE plays a role in the regulation of autophagy in THP-1 human monocytes. SN - 1673-7458 UR - https://www.unboundmedicine.com/medline/citation/21191841/Autoimmune_regulator_regulates_autophagy_in_THP-1_human_monocytes. DB - PRIME DP - Unbound Medicine ER -