Tags

Type your tag names separated by a space and hit enter

Age and 17β-estradiol effects on blood-brain barrier tight junction and estrogen receptor proteins in ovariectomized rats.
Microvasc Res 2011; 81(2):198-205MR

Abstract

Age and estrogen levels alter blood-brain barrier (BBB) tight junction (TJ) regulation, impacting brain homeostasis and pathological outcomes. This examination evaluated BBB TJ and estrogen receptor (ER) protein expression changes in young (8-10 week) and middle-aged (10-12 month) ovariectomized female Fisher-344 rats with chronic 17β-estradiol or placebo treatment. Middle-aged rats showed decreased protein expression of occludin with 17β-estradiol (55 kDa band) or placebo (45, 55, 60 kDa bands) treatment compared to respective young. In young animals, 17β-estradiol treatment increased expression of the occludin 55 kDa band over placebo; however, this effect was lost in the middle-aged animals. In both young and middle-aged animals, expression of claudin-5 (23, 32 kDa bands) and ERα (66 kDa) increased with 17β-estradiol treatment, while junctional adhesion molecule-A showed no change across all groups. However, ERα expression (66 kDa) was significantly reduced in the middle-aged animals compared to young placebo treated animals. Measurement of BBB TJ permeability via in situ perfusion of (14)C-sucrose showed no change with age or treatment. Our results show that increasing age and 17β-estradiol treatment alters the expression of ERα and distinct BBB TJ protein isoforms without altering functional paracellular permeability.

Authors+Show Affiliations

Pharmaceutical Sciences, School of Pharmacy, Southern Illinois University, Edwardsville, 200 University Park Drive, Edwardsville, IL 62026, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21192956

Citation

Sandoval, Karin E., and Ken A. Witt. "Age and 17β-estradiol Effects On Blood-brain Barrier Tight Junction and Estrogen Receptor Proteins in Ovariectomized Rats." Microvascular Research, vol. 81, no. 2, 2011, pp. 198-205.
Sandoval KE, Witt KA. Age and 17β-estradiol effects on blood-brain barrier tight junction and estrogen receptor proteins in ovariectomized rats. Microvasc Res. 2011;81(2):198-205.
Sandoval, K. E., & Witt, K. A. (2011). Age and 17β-estradiol effects on blood-brain barrier tight junction and estrogen receptor proteins in ovariectomized rats. Microvascular Research, 81(2), pp. 198-205. doi:10.1016/j.mvr.2010.12.007.
Sandoval KE, Witt KA. Age and 17β-estradiol Effects On Blood-brain Barrier Tight Junction and Estrogen Receptor Proteins in Ovariectomized Rats. Microvasc Res. 2011;81(2):198-205. PubMed PMID: 21192956.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Age and 17β-estradiol effects on blood-brain barrier tight junction and estrogen receptor proteins in ovariectomized rats. AU - Sandoval,Karin E, AU - Witt,Ken A, Y1 - 2010/12/28/ PY - 2010/08/10/received PY - 2010/11/24/revised PY - 2010/12/20/accepted PY - 2011/1/4/entrez PY - 2011/1/5/pubmed PY - 2011/7/30/medline SP - 198 EP - 205 JF - Microvascular research JO - Microvasc. Res. VL - 81 IS - 2 N2 - Age and estrogen levels alter blood-brain barrier (BBB) tight junction (TJ) regulation, impacting brain homeostasis and pathological outcomes. This examination evaluated BBB TJ and estrogen receptor (ER) protein expression changes in young (8-10 week) and middle-aged (10-12 month) ovariectomized female Fisher-344 rats with chronic 17β-estradiol or placebo treatment. Middle-aged rats showed decreased protein expression of occludin with 17β-estradiol (55 kDa band) or placebo (45, 55, 60 kDa bands) treatment compared to respective young. In young animals, 17β-estradiol treatment increased expression of the occludin 55 kDa band over placebo; however, this effect was lost in the middle-aged animals. In both young and middle-aged animals, expression of claudin-5 (23, 32 kDa bands) and ERα (66 kDa) increased with 17β-estradiol treatment, while junctional adhesion molecule-A showed no change across all groups. However, ERα expression (66 kDa) was significantly reduced in the middle-aged animals compared to young placebo treated animals. Measurement of BBB TJ permeability via in situ perfusion of (14)C-sucrose showed no change with age or treatment. Our results show that increasing age and 17β-estradiol treatment alters the expression of ERα and distinct BBB TJ protein isoforms without altering functional paracellular permeability. SN - 1095-9319 UR - https://www.unboundmedicine.com/medline/citation/21192956/Age_and_17β_estradiol_effects_on_blood_brain_barrier_tight_junction_and_estrogen_receptor_proteins_in_ovariectomized_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0026-2862(10)00264-5 DB - PRIME DP - Unbound Medicine ER -