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Effect of different environmental stresses on the expression of oleate desaturase genes and fatty acid composition in olive fruit.
Phytochemistry. 2011 Feb; 72(2-3):178-87.P

Abstract

The regulation of microsomal and plastidial oleate desaturases by low and high temperature, darkness, and wounding was investigated. To this end, their gene expression levels and the fatty acid composition was determined in the mesocarp tissue of olive fruit from the Picual and Arbequina varieties subjected to the corresponding stress treatments. Firstly, a plastidial oleate desaturase from olive was cloned and its functional identity was confirmed by overexpression in Escherichia coli. The results showed that temperature and light regulate olive oleate desaturase genes at transcriptional level. However, no correlation between their expression levels and the linoleic acid content in microsomal and plastidial lipids was found. In addition, the involvement of microsomal but not plastidial oleate desaturases in the wounding response of olive fruit mesocarp is demonstrated. The fatty acid analysis revealed the appearance of palmitolinoleic acid only in microsomal lipids, reaching a maximum 3h after wounding.

Authors+Show Affiliations

Instituto de la Grasa (CSIC), Sevilla, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21194717

Citation

Hernández, M Luisa, et al. "Effect of Different Environmental Stresses On the Expression of Oleate Desaturase Genes and Fatty Acid Composition in Olive Fruit." Phytochemistry, vol. 72, no. 2-3, 2011, pp. 178-87.
Hernández ML, Padilla MN, Sicardo MD, et al. Effect of different environmental stresses on the expression of oleate desaturase genes and fatty acid composition in olive fruit. Phytochemistry. 2011;72(2-3):178-87.
Hernández, M. L., Padilla, M. N., Sicardo, M. D., Mancha, M., & Martínez-Rivas, J. M. (2011). Effect of different environmental stresses on the expression of oleate desaturase genes and fatty acid composition in olive fruit. Phytochemistry, 72(2-3), 178-87. https://doi.org/10.1016/j.phytochem.2010.11.026
Hernández ML, et al. Effect of Different Environmental Stresses On the Expression of Oleate Desaturase Genes and Fatty Acid Composition in Olive Fruit. Phytochemistry. 2011;72(2-3):178-87. PubMed PMID: 21194717.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of different environmental stresses on the expression of oleate desaturase genes and fatty acid composition in olive fruit. AU - Hernández,M Luisa, AU - Padilla,María N, AU - Sicardo,M Dolores, AU - Mancha,Manuel, AU - Martínez-Rivas,José M, Y1 - 2010/12/29/ PY - 2010/08/02/received PY - 2010/11/24/revised PY - 2010/11/30/accepted PY - 2011/1/4/entrez PY - 2011/1/5/pubmed PY - 2011/3/17/medline SP - 178 EP - 87 JF - Phytochemistry JO - Phytochemistry VL - 72 IS - 2-3 N2 - The regulation of microsomal and plastidial oleate desaturases by low and high temperature, darkness, and wounding was investigated. To this end, their gene expression levels and the fatty acid composition was determined in the mesocarp tissue of olive fruit from the Picual and Arbequina varieties subjected to the corresponding stress treatments. Firstly, a plastidial oleate desaturase from olive was cloned and its functional identity was confirmed by overexpression in Escherichia coli. The results showed that temperature and light regulate olive oleate desaturase genes at transcriptional level. However, no correlation between their expression levels and the linoleic acid content in microsomal and plastidial lipids was found. In addition, the involvement of microsomal but not plastidial oleate desaturases in the wounding response of olive fruit mesocarp is demonstrated. The fatty acid analysis revealed the appearance of palmitolinoleic acid only in microsomal lipids, reaching a maximum 3h after wounding. SN - 1873-3700 UR - https://www.unboundmedicine.com/medline/citation/21194717/Effect_of_different_environmental_stresses_on_the_expression_of_oleate_desaturase_genes_and_fatty_acid_composition_in_olive_fruit_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0031-9422(10)00464-4 DB - PRIME DP - Unbound Medicine ER -