Tags

Type your tag names separated by a space and hit enter

Analysis of global methylation using a Zta-expressing nasopharyngeal carcinoma cell line.
Genomics. 2011 Apr; 97(4):205-13.G

Abstract

EBV infects more than 90% of the human population and persists in most individuals as a latent infection where the viral genome is silenced by host-driven methylation. The lytic cycle is initiated when the viral protein Zta binds to methylated BRLF1 and BRRF1 promoters. Although studies reveal the role of Zta and methylation changes in the viral genome upon EBV infection to reactivation, whether Zta plays any role in alteration of methylation in the host genome remains unknown. Using an inducible model, we demonstrate that global DNA methylation, based on whole-genome 5-methylcytosine content, and regional DNA methylation in repetitive elements, imprinting genes and the X chromosome, remains unchanged in response to Zta expression. Expression of DNA methyltransferases was also unaffected by ectopically expressed Zta. Our data imply that alteration of host gene expression following EBV reactivation may reflect methylation-independent Zta-mediated gene activation and not epigenetic modification of the host genome.

Authors+Show Affiliations

Institute of Basic Medicine, College of Medicine, National Chung Kung University, Tainan, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21195163

Citation

Chen, Ying-Fan, et al. "Analysis of Global Methylation Using a Zta-expressing Nasopharyngeal Carcinoma Cell Line." Genomics, vol. 97, no. 4, 2011, pp. 205-13.
Chen YF, Tung CL, Chang Y, et al. Analysis of global methylation using a Zta-expressing nasopharyngeal carcinoma cell line. Genomics. 2011;97(4):205-13.
Chen, Y. F., Tung, C. L., Chang, Y., Hsiao, W. C., Su, L. J., & Sun, H. S. (2011). Analysis of global methylation using a Zta-expressing nasopharyngeal carcinoma cell line. Genomics, 97(4), 205-13. https://doi.org/10.1016/j.ygeno.2010.12.003
Chen YF, et al. Analysis of Global Methylation Using a Zta-expressing Nasopharyngeal Carcinoma Cell Line. Genomics. 2011;97(4):205-13. PubMed PMID: 21195163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of global methylation using a Zta-expressing nasopharyngeal carcinoma cell line. AU - Chen,Ying-Fan, AU - Tung,Chao-Ling, AU - Chang,Yao, AU - Hsiao,Wen-Chi, AU - Su,Lh-Jen, AU - Sun,H Sunny, Y1 - 2010/12/30/ PY - 2010/11/07/received PY - 2010/12/12/revised PY - 2010/12/14/accepted PY - 2011/1/4/entrez PY - 2011/1/5/pubmed PY - 2011/7/9/medline SP - 205 EP - 13 JF - Genomics JO - Genomics VL - 97 IS - 4 N2 - EBV infects more than 90% of the human population and persists in most individuals as a latent infection where the viral genome is silenced by host-driven methylation. The lytic cycle is initiated when the viral protein Zta binds to methylated BRLF1 and BRRF1 promoters. Although studies reveal the role of Zta and methylation changes in the viral genome upon EBV infection to reactivation, whether Zta plays any role in alteration of methylation in the host genome remains unknown. Using an inducible model, we demonstrate that global DNA methylation, based on whole-genome 5-methylcytosine content, and regional DNA methylation in repetitive elements, imprinting genes and the X chromosome, remains unchanged in response to Zta expression. Expression of DNA methyltransferases was also unaffected by ectopically expressed Zta. Our data imply that alteration of host gene expression following EBV reactivation may reflect methylation-independent Zta-mediated gene activation and not epigenetic modification of the host genome. SN - 1089-8646 UR - https://www.unboundmedicine.com/medline/citation/21195163/Analysis_of_global_methylation_using_a_Zta_expressing_nasopharyngeal_carcinoma_cell_line_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0888-7543(10)00264-8 DB - PRIME DP - Unbound Medicine ER -