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Improvement of the H5N1 influenza virus vaccine strain to decrease the pathogenicity in chicken embryos.
Arch Virol. 2011 Apr; 156(4):557-63.AV

Abstract

The avian influenza vaccine strain A/duck/Hokkaido/Vac-1/2004 (H5N1) (Vac-1) was found to be pathogenic in chicken embryos (CEs). In order to decrease the pathogenicity of Vac-1 in CEs, a series of reassortant viruses was generated between Vac-1 and A/Puerto Rico/8/1934 (H1N1) (PR8), and their pathogenicity and growth potential were compared in CEs. The results indicated that either the PB1 or PA protein was responsible for the pathogenicity of Vac-1 in CEs. The HA titers of the allantoic fluids of CEs inoculated with the recombinant H5N1 viruses, of which pathogenicity was lower than that of the recombinant Vac-1 prepared by reverse genetics in CEs, were equivalent to those of CEs inoculated with the recombinant Vac-1. One of the reassortant viruses, rg-PR8-PA/Vac-1 (H5N1), in which the PA gene was replaced with the corresponding gene of PR8, yielded allantoic fluids with the same HA titer as that of Vac-1, indicating that this reassortant should be a good candidate as an improved vaccine strain.

Authors+Show Affiliations

Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18 Nishi-9, Kita-ku, Sapporo, Hokkaido, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21203786

Citation

Isoda, Norikazu, et al. "Improvement of the H5N1 Influenza Virus Vaccine Strain to Decrease the Pathogenicity in Chicken Embryos." Archives of Virology, vol. 156, no. 4, 2011, pp. 557-63.
Isoda N, Sakoda Y, Okamatsu M, et al. Improvement of the H5N1 influenza virus vaccine strain to decrease the pathogenicity in chicken embryos. Arch Virol. 2011;156(4):557-63.
Isoda, N., Sakoda, Y., Okamatsu, M., Tsuda, Y., & Kida, H. (2011). Improvement of the H5N1 influenza virus vaccine strain to decrease the pathogenicity in chicken embryos. Archives of Virology, 156(4), 557-63. https://doi.org/10.1007/s00705-010-0890-y
Isoda N, et al. Improvement of the H5N1 Influenza Virus Vaccine Strain to Decrease the Pathogenicity in Chicken Embryos. Arch Virol. 2011;156(4):557-63. PubMed PMID: 21203786.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improvement of the H5N1 influenza virus vaccine strain to decrease the pathogenicity in chicken embryos. AU - Isoda,Norikazu, AU - Sakoda,Yoshihiro, AU - Okamatsu,Masatoshi, AU - Tsuda,Yoshimi, AU - Kida,Hiroshi, Y1 - 2011/01/04/ PY - 2010/07/29/received PY - 2010/12/08/accepted PY - 2011/1/5/entrez PY - 2011/1/5/pubmed PY - 2011/5/24/medline SP - 557 EP - 63 JF - Archives of virology JO - Arch Virol VL - 156 IS - 4 N2 - The avian influenza vaccine strain A/duck/Hokkaido/Vac-1/2004 (H5N1) (Vac-1) was found to be pathogenic in chicken embryos (CEs). In order to decrease the pathogenicity of Vac-1 in CEs, a series of reassortant viruses was generated between Vac-1 and A/Puerto Rico/8/1934 (H1N1) (PR8), and their pathogenicity and growth potential were compared in CEs. The results indicated that either the PB1 or PA protein was responsible for the pathogenicity of Vac-1 in CEs. The HA titers of the allantoic fluids of CEs inoculated with the recombinant H5N1 viruses, of which pathogenicity was lower than that of the recombinant Vac-1 prepared by reverse genetics in CEs, were equivalent to those of CEs inoculated with the recombinant Vac-1. One of the reassortant viruses, rg-PR8-PA/Vac-1 (H5N1), in which the PA gene was replaced with the corresponding gene of PR8, yielded allantoic fluids with the same HA titer as that of Vac-1, indicating that this reassortant should be a good candidate as an improved vaccine strain. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/21203786/Improvement_of_the_H5N1_influenza_virus_vaccine_strain_to_decrease_the_pathogenicity_in_chicken_embryos_ L2 - https://dx.doi.org/10.1007/s00705-010-0890-y DB - PRIME DP - Unbound Medicine ER -