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Predominant genera of fecal microbiota in children with atopic dermatitis are not altered by intake of probiotic bacteria Lactobacillus acidophilus NCFM and Bifidobacterium animalis subsp. lactis Bi-07.
FEMS Microbiol Ecol. 2011 Mar; 75(3):482-96.FM

Abstract

The effect of probiotic bacteria Lactobacillus acidophilus NCFM and Bifidobacterium lactis Bi-07 on the composition of the Lactobacillus group, Bifidobacterium and the total bacterial population in feces from young children with atopic dermatitis was investigated. The study included 50 children randomized to intake of one of the probiotic strain or placebo. Microbial composition was characterized by denaturing gradient gel electrophoresis, quantitative PCR and, in a subset of subjects, by pyrosequencing of the 16S rRNA gene. The core population of the Lactobacillus group was identified as Lactobacillus gasseri, Lactobacillus fermentum, Lactobacillus oris, Leuconostoc mesenteroides, while the bifidobacterial community included Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium longum and Bifidobacterium catenulatum. The fecal numbers of L. acidophilus and B. lactis increased significantly after intervention, indicating survival of the ingested bacteria. The levels of Bifidobacterium correlated positively (P=0.03), while the levels of the Lactobacillus group negatively (P=0.01) with improvement of atopic eczema evaluated by the Severity Scoring of Atopic Dermatitis index. This correlation was observed across the whole study cohort and not attributed to the probiotic intake. The main conclusion of the study is that administration of L. acidophilus NCFM and B. lactis Bi-07 does not affect the composition and diversity of the main bacterial populations in feces.

Authors+Show Affiliations

Department of Food Science, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark. nf@life.ku.dkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21204871

Citation

Larsen, Nadja, et al. "Predominant Genera of Fecal Microbiota in Children With Atopic Dermatitis Are Not Altered By Intake of Probiotic Bacteria Lactobacillus Acidophilus NCFM and Bifidobacterium Animalis Subsp. Lactis Bi-07." FEMS Microbiology Ecology, vol. 75, no. 3, 2011, pp. 482-96.
Larsen N, Vogensen FK, Gøbel R, et al. Predominant genera of fecal microbiota in children with atopic dermatitis are not altered by intake of probiotic bacteria Lactobacillus acidophilus NCFM and Bifidobacterium animalis subsp. lactis Bi-07. FEMS Microbiol Ecol. 2011;75(3):482-96.
Larsen, N., Vogensen, F. K., Gøbel, R., Michaelsen, K. F., Abu Al-Soud, W., Sørensen, S. J., Hansen, L. H., & Jakobsen, M. (2011). Predominant genera of fecal microbiota in children with atopic dermatitis are not altered by intake of probiotic bacteria Lactobacillus acidophilus NCFM and Bifidobacterium animalis subsp. lactis Bi-07. FEMS Microbiology Ecology, 75(3), 482-96. https://doi.org/10.1111/j.1574-6941.2010.01024.x
Larsen N, et al. Predominant Genera of Fecal Microbiota in Children With Atopic Dermatitis Are Not Altered By Intake of Probiotic Bacteria Lactobacillus Acidophilus NCFM and Bifidobacterium Animalis Subsp. Lactis Bi-07. FEMS Microbiol Ecol. 2011;75(3):482-96. PubMed PMID: 21204871.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Predominant genera of fecal microbiota in children with atopic dermatitis are not altered by intake of probiotic bacteria Lactobacillus acidophilus NCFM and Bifidobacterium animalis subsp. lactis Bi-07. AU - Larsen,Nadja, AU - Vogensen,Finn K, AU - Gøbel,Rikke, AU - Michaelsen,Kim F, AU - Abu Al-Soud,Waleed, AU - Sørensen,Søren J, AU - Hansen,Lars H, AU - Jakobsen,Mogens, Y1 - 2011/01/11/ PY - 2011/1/6/entrez PY - 2011/1/6/pubmed PY - 2011/2/26/medline SP - 482 EP - 96 JF - FEMS microbiology ecology JO - FEMS Microbiol. Ecol. VL - 75 IS - 3 N2 - The effect of probiotic bacteria Lactobacillus acidophilus NCFM and Bifidobacterium lactis Bi-07 on the composition of the Lactobacillus group, Bifidobacterium and the total bacterial population in feces from young children with atopic dermatitis was investigated. The study included 50 children randomized to intake of one of the probiotic strain or placebo. Microbial composition was characterized by denaturing gradient gel electrophoresis, quantitative PCR and, in a subset of subjects, by pyrosequencing of the 16S rRNA gene. The core population of the Lactobacillus group was identified as Lactobacillus gasseri, Lactobacillus fermentum, Lactobacillus oris, Leuconostoc mesenteroides, while the bifidobacterial community included Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium longum and Bifidobacterium catenulatum. The fecal numbers of L. acidophilus and B. lactis increased significantly after intervention, indicating survival of the ingested bacteria. The levels of Bifidobacterium correlated positively (P=0.03), while the levels of the Lactobacillus group negatively (P=0.01) with improvement of atopic eczema evaluated by the Severity Scoring of Atopic Dermatitis index. This correlation was observed across the whole study cohort and not attributed to the probiotic intake. The main conclusion of the study is that administration of L. acidophilus NCFM and B. lactis Bi-07 does not affect the composition and diversity of the main bacterial populations in feces. SN - 1574-6941 UR - https://www.unboundmedicine.com/medline/citation/21204871/Predominant_genera_of_fecal_microbiota_in_children_with_atopic_dermatitis_are_not_altered_by_intake_of_probiotic_bacteria_Lactobacillus_acidophilus_NCFM_and_Bifidobacterium_animalis_subsp__lactis_Bi_07_ L2 - https://academic.oup.com/femsec/article-lookup/doi/10.1111/j.1574-6941.2010.01024.x DB - PRIME DP - Unbound Medicine ER -