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Methanol is an endogenous elicitor molecule for the synthesis of tryptophan and tryptophan-derived secondary metabolites upon senescence of detached rice leaves.
Plant J. 2011 Apr; 66(2):247-57.PJ

Abstract

During senescence of detached rice leaves, tryptophan (Trp) and Trp-derived secondary metabolites such as serotonin and 4-coumaroylserotonin accumulated in concert with methanol (MeOH) production. This senescence-induced MeOH induction was closely associated with levels of pectin methylesterase (PME)1 mRNA and PME enzyme activity. Exogenous challenge of detached rice leaves with 1% MeOH accelerated Trp and serotonin biosynthesis with induction of the corresponding genes. No other solvents, including ethanol, resulted in a Trp-inducing effect. This MeOH-induced Trp synthesis was positively regulated by abscisic acid but negatively regulated by cytokinin, suggesting hormonal involvement in the action of MeOH. Endogenous overproduction or suppression of MeOH either by PME1 overexpression or RNA interference (RNAi) gene silencing revealed that PME1 overexpressing lines produced twofold higher Trp levels with elevated Trp biosynthetic gene expression, whereas RNAi lines showed twofold reduction in Trp level in healthy control rice leaves, suggesting that MeOH acts as an endogenous elicitor to enhance Trp biosynthesis. Among many transcription factors induced following MeOH treatment, the WRKY family showed significant induction patterns, of which WRKY14 appeared to play a key regulatory role in MeOH-induced Trp and Trp-derived secondary metabolite biosynthesis.

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Authors+Show Affiliations

Kang K
Department of Biotechnology, Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Bioenergy Research Center, Chonnam National University, Gwangju 500-757, Republic of Korea.
Park S
No affiliation info available
Natsagdorj U
No affiliation info available
Kim YS
No affiliation info available
Back K
No affiliation info available

MeSH

Abscisic AcidCarboxylic Ester HydrolasesMethanolOryzaPlant LeavesPlant ProteinsPlants, Genetically ModifiedRNA InterferenceSerotoninTranscription FactorsTryptophan

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21205035

Citation

Kang, Kiyoon, et al. "Methanol Is an Endogenous Elicitor Molecule for the Synthesis of Tryptophan and Tryptophan-derived Secondary Metabolites Upon Senescence of Detached Rice Leaves." The Plant Journal : for Cell and Molecular Biology, vol. 66, no. 2, 2011, pp. 247-57.
Kang K, Park S, Natsagdorj U, et al. Methanol is an endogenous elicitor molecule for the synthesis of tryptophan and tryptophan-derived secondary metabolites upon senescence of detached rice leaves. Plant J. 2011;66(2):247-57.
Kang, K., Park, S., Natsagdorj, U., Kim, Y. S., & Back, K. (2011). Methanol is an endogenous elicitor molecule for the synthesis of tryptophan and tryptophan-derived secondary metabolites upon senescence of detached rice leaves. The Plant Journal : for Cell and Molecular Biology, 66(2), 247-57. https://doi.org/10.1111/j.1365-313X.2011.04486.x
Kang K, et al. Methanol Is an Endogenous Elicitor Molecule for the Synthesis of Tryptophan and Tryptophan-derived Secondary Metabolites Upon Senescence of Detached Rice Leaves. Plant J. 2011;66(2):247-57. PubMed PMID: 21205035.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Methanol is an endogenous elicitor molecule for the synthesis of tryptophan and tryptophan-derived secondary metabolites upon senescence of detached rice leaves. AU - Kang,Kiyoon, AU - Park,Sangkyu, AU - Natsagdorj,Uyanga, AU - Kim,Young Soon, AU - Back,Kyoungwhan, Y1 - 2011/02/18/ PY - 2011/1/6/entrez PY - 2011/1/6/pubmed PY - 2011/7/29/medline SP - 247 EP - 57 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 66 IS - 2 N2 - During senescence of detached rice leaves, tryptophan (Trp) and Trp-derived secondary metabolites such as serotonin and 4-coumaroylserotonin accumulated in concert with methanol (MeOH) production. This senescence-induced MeOH induction was closely associated with levels of pectin methylesterase (PME)1 mRNA and PME enzyme activity. Exogenous challenge of detached rice leaves with 1% MeOH accelerated Trp and serotonin biosynthesis with induction of the corresponding genes. No other solvents, including ethanol, resulted in a Trp-inducing effect. This MeOH-induced Trp synthesis was positively regulated by abscisic acid but negatively regulated by cytokinin, suggesting hormonal involvement in the action of MeOH. Endogenous overproduction or suppression of MeOH either by PME1 overexpression or RNA interference (RNAi) gene silencing revealed that PME1 overexpressing lines produced twofold higher Trp levels with elevated Trp biosynthetic gene expression, whereas RNAi lines showed twofold reduction in Trp level in healthy control rice leaves, suggesting that MeOH acts as an endogenous elicitor to enhance Trp biosynthesis. Among many transcription factors induced following MeOH treatment, the WRKY family showed significant induction patterns, of which WRKY14 appeared to play a key regulatory role in MeOH-induced Trp and Trp-derived secondary metabolite biosynthesis. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/21205035/Methanol_is_an_endogenous_elicitor_molecule_for_the_synthesis_of_tryptophan_and_tryptophan_derived_secondary_metabolites_upon_senescence_of_detached_rice_leaves_ L2 - https://doi.org/10.1111/j.1365-313X.2011.04486.x DB - PRIME DP - Unbound Medicine ER -