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Crystallization and preliminary X-ray crystallographic analysis of human quinolinate phosphoribosyltransferase.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jan 01; 67(Pt 1):38-40.AC

Abstract

Quinolinate phosphoribosyltransferase (QPRTase) is a key NAD-biosynthetic enzyme which catalyzes the transfer of quinolinic acid to 5-phosphoribosyl-1-pyrophosphate, yielding nicotinic acid mononucleotide. Homo sapiens QPRTase (Hs-QPRTase) appeared as a hexamer during purification and the protein was crystallized. Diffraction data were collected and processed at 2.8 Å resolution. Native Hs-QPRTase crystals belonged to space group P2(1), with unit-cell parameters a=76.2, b=137.1, c=92.7 Å, β=103.8°. Assuming the presence of six molecules in the asymmetric unit, the calculated Matthews coefficient is 2.46 Å3 Da(-1), which corresponds to a solvent content of 49.9%.

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Authors+Show Affiliations

Kang GB
School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
Kim MK
No affiliation info available
Youn HS
No affiliation info available
An JY
No affiliation info available
Lee JG
No affiliation info available
Park KR
No affiliation info available
Lee SH
No affiliation info available
Kim Y
No affiliation info available
Fukuoka S
No affiliation info available
Eom SH
No affiliation info available

MeSH

AnimalsCrystallizationCrystallography, X-RayHumansMolecular Sequence DataNADPentosyltransferasesProtein Structure, Quaternary

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21206019

Citation

Kang, Gil Bu, et al. "Crystallization and Preliminary X-ray Crystallographic Analysis of Human Quinolinate Phosphoribosyltransferase." Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, vol. 67, no. Pt 1, 2011, pp. 38-40.
Kang GB, Kim MK, Youn HS, et al. Crystallization and preliminary X-ray crystallographic analysis of human quinolinate phosphoribosyltransferase. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67(Pt 1):38-40.
Kang, G. B., Kim, M. K., Youn, H. S., An, J. Y., Lee, J. G., Park, K. R., Lee, S. H., Kim, Y., Fukuoka, S., & Eom, S. H. (2011). Crystallization and preliminary X-ray crystallographic analysis of human quinolinate phosphoribosyltransferase. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 67(Pt 1), 38-40. https://doi.org/10.1107/S1744309110041011
Kang GB, et al. Crystallization and Preliminary X-ray Crystallographic Analysis of Human Quinolinate Phosphoribosyltransferase. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jan 1;67(Pt 1):38-40. PubMed PMID: 21206019.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crystallization and preliminary X-ray crystallographic analysis of human quinolinate phosphoribosyltransferase. AU - Kang,Gil Bu, AU - Kim,Mun-Kyoung, AU - Youn,Hyung-Seop, AU - An,Jun Yop, AU - Lee,Jung-Gyu, AU - Park,Kyoung Ryoung, AU - Lee,Sung Hang, AU - Kim,Yongseong, AU - Fukuoka,Shin-Ichi, AU - Eom,Soo Hyun, Y1 - 2010/12/21/ PY - 2010/01/20/received PY - 2010/10/12/accepted PY - 2011/1/6/entrez PY - 2011/1/6/pubmed PY - 2011/4/13/medline SP - 38 EP - 40 JF - Acta crystallographica. Section F, Structural biology and crystallization communications JO - Acta Crystallogr Sect F Struct Biol Cryst Commun VL - 67 IS - Pt 1 N2 - Quinolinate phosphoribosyltransferase (QPRTase) is a key NAD-biosynthetic enzyme which catalyzes the transfer of quinolinic acid to 5-phosphoribosyl-1-pyrophosphate, yielding nicotinic acid mononucleotide. Homo sapiens QPRTase (Hs-QPRTase) appeared as a hexamer during purification and the protein was crystallized. Diffraction data were collected and processed at 2.8 Å resolution. Native Hs-QPRTase crystals belonged to space group P2(1), with unit-cell parameters a=76.2, b=137.1, c=92.7 Å, β=103.8°. Assuming the presence of six molecules in the asymmetric unit, the calculated Matthews coefficient is 2.46 Å3 Da(-1), which corresponds to a solvent content of 49.9%. SN - 1744-3091 UR - https://www.unboundmedicine.com/medline/citation/21206019/Crystallization_and_preliminary_X_ray_crystallographic_analysis_of_human_quinolinate_phosphoribosyltransferase_ L2 - http://scripts.iucr.org/cgi-bin/paper?S1744309110041011 DB - PRIME DP - Unbound Medicine ER -