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Dependence of hemoglobin mass estimation with the optimized CO-rebreathing method on different spectrophotometers.
Scand J Med Sci Sports. 2012 Apr; 22(2):224-31.SJ

Abstract

The assessment of total hemoglobin mass (tHb-mass) with the optimized carbon monoxide-rebreathing procedure (oCOR) is discussed as a promising method to detect blood doping. The method requires repeated measurements of the carboxyhemoglobin fraction (%HbCO) using spectrophotometers (CO oximeters). In order to determine whether %HbCO measurements with different spectrophotometers yield similar tHb-masses, the results of 57 tHb-mass calculations from simultaneous %HbCO measurements with two different spectrophotometers (RapidLab, OSM3) were analyzed. For the comparison of longitudinal tHb-mass alterations (ΔtHb-mass), 3 tHb-mass measurements were obtained at 6-month intervals (33-37 subjects). Because of significant differences in %HbCO measurements, the limits of agreement for tHb-mass(OSM3) and tHb-mass(RapidLab) were 11.2% (95% reference range -6.8 to +15.6%) and the correlation of ΔtHb-masses as determined with the two spectrophotometers over two time intervals was weak (r: 0.28-0.66). In only about 70% of all ΔtHb-mass estimations did ΔtHb-mass(OSM3) and ΔtHb-mass(RapidLab) show the same direction of change. Apparently, the analytical variation in tHb-mass determination with oCOR increases considerably with the use of different spectrophotometers. Therefore, agreement on the use of one spectrophotometer that accurately measures low %HbCO values is needed if oCOR should be used in an anti-doping setting.

Authors+Show Affiliations

Department of Sports Medicine, Medical Clinic, University Hospital Heidelberg, Heidelberg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

21210851

Citation

Ulrich, G, et al. "Dependence of Hemoglobin Mass Estimation With the Optimized CO-rebreathing Method On Different Spectrophotometers." Scandinavian Journal of Medicine & Science in Sports, vol. 22, no. 2, 2012, pp. 224-31.
Ulrich G, Strunz J, Frese F, et al. Dependence of hemoglobin mass estimation with the optimized CO-rebreathing method on different spectrophotometers. Scand J Med Sci Sports. 2012;22(2):224-31.
Ulrich, G., Strunz, J., Frese, F., Bärtsch, P., & Friedmann-Bette, B. (2012). Dependence of hemoglobin mass estimation with the optimized CO-rebreathing method on different spectrophotometers. Scandinavian Journal of Medicine & Science in Sports, 22(2), 224-31. https://doi.org/10.1111/j.1600-0838.2010.01247.x
Ulrich G, et al. Dependence of Hemoglobin Mass Estimation With the Optimized CO-rebreathing Method On Different Spectrophotometers. Scand J Med Sci Sports. 2012;22(2):224-31. PubMed PMID: 21210851.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dependence of hemoglobin mass estimation with the optimized CO-rebreathing method on different spectrophotometers. AU - Ulrich,G, AU - Strunz,J, AU - Frese,F, AU - Bärtsch,P, AU - Friedmann-Bette,B, Y1 - 2011/01/07/ PY - 2011/1/8/entrez PY - 2011/1/8/pubmed PY - 2012/7/10/medline SP - 224 EP - 31 JF - Scandinavian journal of medicine & science in sports JO - Scand J Med Sci Sports VL - 22 IS - 2 N2 - The assessment of total hemoglobin mass (tHb-mass) with the optimized carbon monoxide-rebreathing procedure (oCOR) is discussed as a promising method to detect blood doping. The method requires repeated measurements of the carboxyhemoglobin fraction (%HbCO) using spectrophotometers (CO oximeters). In order to determine whether %HbCO measurements with different spectrophotometers yield similar tHb-masses, the results of 57 tHb-mass calculations from simultaneous %HbCO measurements with two different spectrophotometers (RapidLab, OSM3) were analyzed. For the comparison of longitudinal tHb-mass alterations (ΔtHb-mass), 3 tHb-mass measurements were obtained at 6-month intervals (33-37 subjects). Because of significant differences in %HbCO measurements, the limits of agreement for tHb-mass(OSM3) and tHb-mass(RapidLab) were 11.2% (95% reference range -6.8 to +15.6%) and the correlation of ΔtHb-masses as determined with the two spectrophotometers over two time intervals was weak (r: 0.28-0.66). In only about 70% of all ΔtHb-mass estimations did ΔtHb-mass(OSM3) and ΔtHb-mass(RapidLab) show the same direction of change. Apparently, the analytical variation in tHb-mass determination with oCOR increases considerably with the use of different spectrophotometers. Therefore, agreement on the use of one spectrophotometer that accurately measures low %HbCO values is needed if oCOR should be used in an anti-doping setting. SN - 1600-0838 UR - https://www.unboundmedicine.com/medline/citation/21210851/Dependence_of_hemoglobin_mass_estimation_with_the_optimized_CO_rebreathing_method_on_different_spectrophotometers_ L2 - https://doi.org/10.1111/j.1600-0838.2010.01247.x DB - PRIME DP - Unbound Medicine ER -