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[Molecular genetic features of sporadic Burkitt's lymphoma in children].
Zhonghua Bing Li Xue Za Zhi 2010; 39(12):819-24ZB

Abstract

OBJECTIVE

To investigate the molecular genetic features and diagnostic aspects of sporadic Burkitt's lymphoma (BL) in children.

METHODS

Tissue microarray was constructed to include 64 cases of pediatric BL and 6 cases of pediatric diffuse large B-cell lymphoma (DLBCL). Immunohistochemistry and fluorescence in-situ hybridization for c-myc, bcl-2, bcl-6, IgH, myc/IgH and bcl-2/IgH gene were performed. Cases of pediatric Burkitt's lymphomas were subclassified into three groups based on their cellular orgins: the germinal center (GC) group, the late-germinal center (late-GC) group and the post-germinal center (post-GC) group.

RESULTS

Among 64 Burkitt's lymphomas studied, expression of CD20, CD10, bcl-6, bcl-2 and MUM1 by immunohistochemistry were 100% (64 cases), 98.4% (63 cases), 96.9% (62 cases), 0 (0 cases) and 23.4% (15 cases), respectively. Various gene rearrangements were found involving the c-myc 93.1% (54/58 cases) and IgH 82.8% (48/58 cases). Detailed rearrangements are as follows: 46 cases (85.2%) myc/IgH gene translocation along with c-myc and IgH gene rearrangement; 4 cases (7.4%) c-myc gene rearrangement without IgH and myc/IgH abnormality; 4 cases (7.4%) without c-myc, IgH or myc/IgH gene rearrangement. No case showed bcl-2 gene abnormality (100%). Fifty nine cases showed normal bcl-6 gene status. One case had bcl-6 gene rearrangement and amplification with the pathologic and immunophenotypic characteristics of BL, leading to a revised pathological diagnosis of B-cell lymphoma, unclassifiable with features intermediate between diffuse large B-cell lymphoma and Burkitt's lymphoma (DLBCL/BL). Two cases showed c-myc gene rearrangement. Two cases showed bcl-6 gene amplification and 6 DLBCL cases had a normal status of bcl-2/IgH.

CONCLUSIONS

A majority of pediatric sporadic BL arise from the germinal center B cells, most of which have c-myc gene rearrangement. It is useful to distinguish BL and DLBCL by multiple genes detection.

Authors+Show Affiliations

Department of Pathology, Jiangxi Children's Hospital, Nanchang 330006, China. ywp07912000@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

21215097

Citation

Yang, Wen-Ping, et al. "[Molecular Genetic Features of Sporadic Burkitt's Lymphoma in Children]." Zhonghua Bing Li Xue Za Zhi = Chinese Journal of Pathology, vol. 39, no. 12, 2010, pp. 819-24.
Yang WP, Huang H, Gong LP, et al. [Molecular genetic features of sporadic Burkitt's lymphoma in children]. Zhonghua Bing Li Xue Za Zhi. 2010;39(12):819-24.
Yang, W. P., Huang, H., Gong, L. P., Wu, Y., Xu, H. Y., Zou, Y., ... Zhu, C. D. (2010). [Molecular genetic features of sporadic Burkitt's lymphoma in children]. Zhonghua Bing Li Xue Za Zhi = Chinese Journal of Pathology, 39(12), pp. 819-24.
Yang WP, et al. [Molecular Genetic Features of Sporadic Burkitt's Lymphoma in Children]. Zhonghua Bing Li Xue Za Zhi. 2010;39(12):819-24. PubMed PMID: 21215097.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Molecular genetic features of sporadic Burkitt's lymphoma in children]. AU - Yang,Wen-Ping, AU - Huang,Hui, AU - Gong,Li-Ping, AU - Wu,Yan, AU - Xu,Hong-Yan, AU - Zou,Yin, AU - Lü,Bei-Bei, AU - Zhong,Hua-Sheng, AU - Deng,Qing-Qiang, AU - Xiao,Qiang, AU - Zeng,Song-Tao, AU - Zhu,Cai-di, PY - 2011/1/11/entrez PY - 2011/1/11/pubmed PY - 2011/9/20/medline SP - 819 EP - 24 JF - Zhonghua bing li xue za zhi = Chinese journal of pathology JO - Zhonghua Bing Li Xue Za Zhi VL - 39 IS - 12 N2 - OBJECTIVE: To investigate the molecular genetic features and diagnostic aspects of sporadic Burkitt's lymphoma (BL) in children. METHODS: Tissue microarray was constructed to include 64 cases of pediatric BL and 6 cases of pediatric diffuse large B-cell lymphoma (DLBCL). Immunohistochemistry and fluorescence in-situ hybridization for c-myc, bcl-2, bcl-6, IgH, myc/IgH and bcl-2/IgH gene were performed. Cases of pediatric Burkitt's lymphomas were subclassified into three groups based on their cellular orgins: the germinal center (GC) group, the late-germinal center (late-GC) group and the post-germinal center (post-GC) group. RESULTS: Among 64 Burkitt's lymphomas studied, expression of CD20, CD10, bcl-6, bcl-2 and MUM1 by immunohistochemistry were 100% (64 cases), 98.4% (63 cases), 96.9% (62 cases), 0 (0 cases) and 23.4% (15 cases), respectively. Various gene rearrangements were found involving the c-myc 93.1% (54/58 cases) and IgH 82.8% (48/58 cases). Detailed rearrangements are as follows: 46 cases (85.2%) myc/IgH gene translocation along with c-myc and IgH gene rearrangement; 4 cases (7.4%) c-myc gene rearrangement without IgH and myc/IgH abnormality; 4 cases (7.4%) without c-myc, IgH or myc/IgH gene rearrangement. No case showed bcl-2 gene abnormality (100%). Fifty nine cases showed normal bcl-6 gene status. One case had bcl-6 gene rearrangement and amplification with the pathologic and immunophenotypic characteristics of BL, leading to a revised pathological diagnosis of B-cell lymphoma, unclassifiable with features intermediate between diffuse large B-cell lymphoma and Burkitt's lymphoma (DLBCL/BL). Two cases showed c-myc gene rearrangement. Two cases showed bcl-6 gene amplification and 6 DLBCL cases had a normal status of bcl-2/IgH. CONCLUSIONS: A majority of pediatric sporadic BL arise from the germinal center B cells, most of which have c-myc gene rearrangement. It is useful to distinguish BL and DLBCL by multiple genes detection. SN - 0529-5807 UR - https://www.unboundmedicine.com/medline/citation/21215097/[Molecular_genetic_features_of_sporadic_Burkitt's_lymphoma_in_children]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0529-5807&year=2010&vol=39&issue=12&fpage=819 DB - PRIME DP - Unbound Medicine ER -