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Differences in binding affinities of MDA, MDMA, MDEA, Amphetamine, Methamphetamine, and their deuterated analogues to solid-phase extraction cartridges.
J Anal Toxicol. 2011 Jan; 35(1):15-22.JA

Abstract

This study evaluated the potential for partial separation of drugs from their deuterated internal standards using Cerex(®) Polycrom™ CLIN II solid-phase extraction (SPE) cartridges. After elution from the column and derivatization, gas chromatography-mass spectrometry results showed that the target compound eluted from the SPE cartridge prior to its deuterated form. This elution separation effect was greater for 3,4-methylenedioxymethamphetamine (MDMA) and methamphetamine (MAMP) than for the other drugs studied. When the drugs were eluted in 0.5 mL increments from a 50 mg sorbent bed, no drug appeared in the first fraction. The drug to internal standard ratios (expected value 1.00) for subsequent fractions collected were 1.30, 1.07, and 0.83 for MDA/MDA-d(5); 1.65, 1.18, 0.67, and 0.56 for MDMA/MDMAd(5); and 1.37, 1.18, and 0.95 for MDEA/MDEA-d(6). For d-AMP and d-MAMP, the expected ratio was 0.40. The subsequent ratios were 0.63, 0.46, 0.35, and 0.34 for d-AMP/d-AMP-d(11); and 1.00, 0.59, 0.25, and 0.18 for d-MAMP/d-MAMP-d(14). The affinity of d-MAMPd(14) was shown to be greater than that of d-MAMP-d(5), and deuteration at the propyl end of the molecule was shown to increase binding more than deuteration on the phenyl group.

Authors+Show Affiliations

Navy Drug Screening Laboratory, 320B B Street, Great Lakes, Illinois, USA. robert.romberg@med.navy.milNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

21219698

Citation

Romberg, R W., et al. "Differences in Binding Affinities of MDA, MDMA, MDEA, Amphetamine, Methamphetamine, and Their Deuterated Analogues to Solid-phase Extraction Cartridges." Journal of Analytical Toxicology, vol. 35, no. 1, 2011, pp. 15-22.
Romberg RW, Ntamack AG, Blacik LJ, et al. Differences in binding affinities of MDA, MDMA, MDEA, Amphetamine, Methamphetamine, and their deuterated analogues to solid-phase extraction cartridges. J Anal Toxicol. 2011;35(1):15-22.
Romberg, R. W., Ntamack, A. G., Blacik, L. J., Kazarian, C. M., Snyder, J. J., & Welsh, E. R. (2011). Differences in binding affinities of MDA, MDMA, MDEA, Amphetamine, Methamphetamine, and their deuterated analogues to solid-phase extraction cartridges. Journal of Analytical Toxicology, 35(1), 15-22.
Romberg RW, et al. Differences in Binding Affinities of MDA, MDMA, MDEA, Amphetamine, Methamphetamine, and Their Deuterated Analogues to Solid-phase Extraction Cartridges. J Anal Toxicol. 2011;35(1):15-22. PubMed PMID: 21219698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differences in binding affinities of MDA, MDMA, MDEA, Amphetamine, Methamphetamine, and their deuterated analogues to solid-phase extraction cartridges. AU - Romberg,R W, AU - Ntamack,A G, AU - Blacik,L J, AU - Kazarian,C M, AU - Snyder,J Jacob, AU - Welsh,E R, PY - 2011/1/12/entrez PY - 2011/1/12/pubmed PY - 2011/4/19/medline SP - 15 EP - 22 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 35 IS - 1 N2 - This study evaluated the potential for partial separation of drugs from their deuterated internal standards using Cerex(®) Polycrom™ CLIN II solid-phase extraction (SPE) cartridges. After elution from the column and derivatization, gas chromatography-mass spectrometry results showed that the target compound eluted from the SPE cartridge prior to its deuterated form. This elution separation effect was greater for 3,4-methylenedioxymethamphetamine (MDMA) and methamphetamine (MAMP) than for the other drugs studied. When the drugs were eluted in 0.5 mL increments from a 50 mg sorbent bed, no drug appeared in the first fraction. The drug to internal standard ratios (expected value 1.00) for subsequent fractions collected were 1.30, 1.07, and 0.83 for MDA/MDA-d(5); 1.65, 1.18, 0.67, and 0.56 for MDMA/MDMAd(5); and 1.37, 1.18, and 0.95 for MDEA/MDEA-d(6). For d-AMP and d-MAMP, the expected ratio was 0.40. The subsequent ratios were 0.63, 0.46, 0.35, and 0.34 for d-AMP/d-AMP-d(11); and 1.00, 0.59, 0.25, and 0.18 for d-MAMP/d-MAMP-d(14). The affinity of d-MAMPd(14) was shown to be greater than that of d-MAMP-d(5), and deuteration at the propyl end of the molecule was shown to increase binding more than deuteration on the phenyl group. SN - 1945-2403 UR - https://www.unboundmedicine.com/medline/citation/21219698/Differences_in_binding_affinities_of_MDA_MDMA_MDEA_Amphetamine_Methamphetamine_and_their_deuterated_analogues_to_solid_phase_extraction_cartridges_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/anatox/35.1.15 DB - PRIME DP - Unbound Medicine ER -