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Toxigenic status of Staphylococcus aureus isolated from bovine raw milk and Minas frescal cheese in Brazil.
J Food Prot. 2010 Dec; 73(12):2225-31.JF

Abstract

A group of 291 Staphylococcus aureus isolates from mastitic cow's milk (n = 125), bulk tank milk (n = 96), and Minas frescal cheese (n = 70) were screened for staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed, see, seg, seh, sei, selj, and sell) and for the tst-1 gene encoding staphylococcal toxic shock syndrome toxin 1 by PCR assay. A total of 109 (37.5%) of the isolates were positive for at least one of these 11 genes, and 23 distinct genotypes of toxin genes were observed. Of the S. aureus isolates bearing SE genes, 17 (13.6%) were from mastitic cow's milk, 41 (41.7%) were from bulk tank milk, and 51 (72.9%) were from Minas frescal cheese. The occurrence of exclusively more recently described SE genes (seg through sell) was considerably higher (87 of 109 PCR-positive strains) than that of classical SE genes (sea through see, 15 strains). The SE genes most commonly detected were seg and sei; they were found alone or in different combinations with other toxin genes, but in 60.8% of the cases they were codetected. No strain possessed see. The tst-1 gene was found in eight isolates but none from mastitic cow's milk. Macrorestriction analysis of chromosomal DNA from 89 S. aureus isolates positive for SE gene(s) was conducted with the enzyme SmaI. Fifty-five distinct pulsed-field gel electrophoresis patterns were found, demonstrating a lack of predominance of any specific clone. A second enzyme, Apa I, used for some isolates was less discriminating than Sma I. The high genotype diversity of potential toxigenic S. aureus strains found in this study, especially from Minas frescal cheese, suggests various sources of contamination. Efforts from the entire production chain are required to improve consumer safety.

Authors+Show Affiliations

Embrapa Dairy Cattle, Rua Eugenio do Nascimento 610, Bairro Dom Bosco, 36038-330, Juiz de Fora, Minas Gerais, Brazil. edna@cnpgl.embrapa.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21219740

Citation

Arcuri, Edna Froeder, et al. "Toxigenic Status of Staphylococcus Aureus Isolated From Bovine Raw Milk and Minas Frescal Cheese in Brazil." Journal of Food Protection, vol. 73, no. 12, 2010, pp. 2225-31.
Arcuri EF, Angelo FF, Guimarães MF, et al. Toxigenic status of Staphylococcus aureus isolated from bovine raw milk and Minas frescal cheese in Brazil. J Food Prot. 2010;73(12):2225-31.
Arcuri, E. F., Angelo, F. F., Guimarães, M. F., Talon, R., Borges, M. d. e. . F., Leroy, S., Loiseau, G., Lange, C. C., Andrade, N. J., & Montet, D. (2010). Toxigenic status of Staphylococcus aureus isolated from bovine raw milk and Minas frescal cheese in Brazil. Journal of Food Protection, 73(12), 2225-31.
Arcuri EF, et al. Toxigenic Status of Staphylococcus Aureus Isolated From Bovine Raw Milk and Minas Frescal Cheese in Brazil. J Food Prot. 2010;73(12):2225-31. PubMed PMID: 21219740.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Toxigenic status of Staphylococcus aureus isolated from bovine raw milk and Minas frescal cheese in Brazil. AU - Arcuri,Edna Froeder, AU - Angelo,Fabiola Fonseca, AU - Guimarães,Marta Fonseca Martins, AU - Talon,Régine, AU - Borges,Maria de Fatima, AU - Leroy,Sabine, AU - Loiseau,Gérard, AU - Lange,Carla Christine, AU - Andrade,Nélio José de, AU - Montet,Didier, PY - 2011/1/12/entrez PY - 2011/1/12/pubmed PY - 2011/2/1/medline SP - 2225 EP - 31 JF - Journal of food protection JO - J Food Prot VL - 73 IS - 12 N2 - A group of 291 Staphylococcus aureus isolates from mastitic cow's milk (n = 125), bulk tank milk (n = 96), and Minas frescal cheese (n = 70) were screened for staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed, see, seg, seh, sei, selj, and sell) and for the tst-1 gene encoding staphylococcal toxic shock syndrome toxin 1 by PCR assay. A total of 109 (37.5%) of the isolates were positive for at least one of these 11 genes, and 23 distinct genotypes of toxin genes were observed. Of the S. aureus isolates bearing SE genes, 17 (13.6%) were from mastitic cow's milk, 41 (41.7%) were from bulk tank milk, and 51 (72.9%) were from Minas frescal cheese. The occurrence of exclusively more recently described SE genes (seg through sell) was considerably higher (87 of 109 PCR-positive strains) than that of classical SE genes (sea through see, 15 strains). The SE genes most commonly detected were seg and sei; they were found alone or in different combinations with other toxin genes, but in 60.8% of the cases they were codetected. No strain possessed see. The tst-1 gene was found in eight isolates but none from mastitic cow's milk. Macrorestriction analysis of chromosomal DNA from 89 S. aureus isolates positive for SE gene(s) was conducted with the enzyme SmaI. Fifty-five distinct pulsed-field gel electrophoresis patterns were found, demonstrating a lack of predominance of any specific clone. A second enzyme, Apa I, used for some isolates was less discriminating than Sma I. The high genotype diversity of potential toxigenic S. aureus strains found in this study, especially from Minas frescal cheese, suggests various sources of contamination. Efforts from the entire production chain are required to improve consumer safety. SN - 1944-9097 UR - https://www.unboundmedicine.com/medline/citation/21219740/Toxigenic_status_of_Staphylococcus_aureus_isolated_from_bovine_raw_milk_and_Minas_frescal_cheese_in_Brazil_ L2 - https://meridian.allenpress.com/jfp/article-lookup/doi/10.4315/0362-028x-73.12.2225 DB - PRIME DP - Unbound Medicine ER -