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Expression of ER quality control-related genes in response to changes in BiP1 levels in developing rice endosperm.
Plant J. 2011 Mar; 65(5):675-89.PJ

Abstract

Binding protein (BiP) is the key chaperone involved in folding of secretory proteins such as seed storage proteins in the ER lumen. To obtain functional information about BiP1, a gene that is predominantly expressed during rice seed maturation, we generated several transgenic rice plants in which various levels of BiP1 protein accumulated in an endosperm-specific manner. Severe suppression (BiP1 KD) or significant over-expression (BiP1 OEmax) of BiP1 not only altered seed phenotype and the intracellular structure of endosperm cells, but also reduced seed storage protein content, starch accumulation and grain weight. Microarray and RT-PCR analyses indicated that expression of many chaperone and co-chaperone genes was induced in transgenic plants, with more prominent expression in the BiP1 KD line than in the BiP1 OEmax line. Transcriptional induction of most chaperones was observed in calli treated with dithiothreitol or tunicamycin, treatments that trigger ER stress, indicating that induction of the chaperone genes in transgenic rice was caused by an ER stress response. In transient assays using rice protoplasts, the ortholog (Os06g0622700) of the AtbZIP60 transcription factor was shown to be involved in activation of some chaperone genes. Slight increases in the BiP1 level compared with wild-type, accompanied by increased levels of calnexin and protein disulfide isomerase-like proteins, resulted in significant enhancement of seed storage protein content, without any change in intracellular structure or seed phenotype. Judicious modification of BiP1 levels in transgenic rice can provide suitable conditions for the production of secretory proteins by alleviating ER stress.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wakasa Y
Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan.
Yasuda H
No affiliation info available
Oono Y
No affiliation info available
Kawakatsu T
No affiliation info available
Hirose S
No affiliation info available
Takahashi H
No affiliation info available
Hayashi S
No affiliation info available
Yang L
No affiliation info available
Takaiwa F
No affiliation info available

MeSH

Basic-Leucine Zipper Transcription FactorsEndoplasmic ReticulumEndospermGene Expression ProfilingGene Expression Regulation, PlantMolecular ChaperonesOligonucleotide Array Sequence AnalysisOryzaPlant ProteinsPlants, Genetically ModifiedRNA, PlantSeeds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21223397

Citation

Wakasa, Yuhya, et al. "Expression of ER Quality Control-related Genes in Response to Changes in BiP1 Levels in Developing Rice Endosperm." The Plant Journal : for Cell and Molecular Biology, vol. 65, no. 5, 2011, pp. 675-89.
Wakasa Y, Yasuda H, Oono Y, et al. Expression of ER quality control-related genes in response to changes in BiP1 levels in developing rice endosperm. Plant J. 2011;65(5):675-89.
Wakasa, Y., Yasuda, H., Oono, Y., Kawakatsu, T., Hirose, S., Takahashi, H., Hayashi, S., Yang, L., & Takaiwa, F. (2011). Expression of ER quality control-related genes in response to changes in BiP1 levels in developing rice endosperm. The Plant Journal : for Cell and Molecular Biology, 65(5), 675-89. https://doi.org/10.1111/j.1365-313X.2010.04453.x
Wakasa Y, et al. Expression of ER Quality Control-related Genes in Response to Changes in BiP1 Levels in Developing Rice Endosperm. Plant J. 2011;65(5):675-89. PubMed PMID: 21223397.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of ER quality control-related genes in response to changes in BiP1 levels in developing rice endosperm. AU - Wakasa,Yuhya, AU - Yasuda,Hiroshi, AU - Oono,Youko, AU - Kawakatsu,Taiji, AU - Hirose,Sakiko, AU - Takahashi,Hideyuki, AU - Hayashi,Shimpei, AU - Yang,Lijun, AU - Takaiwa,Fumio, Y1 - 2011/01/12/ PY - 2011/1/13/entrez PY - 2011/1/13/pubmed PY - 2011/6/18/medline SP - 675 EP - 89 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 65 IS - 5 N2 - Binding protein (BiP) is the key chaperone involved in folding of secretory proteins such as seed storage proteins in the ER lumen. To obtain functional information about BiP1, a gene that is predominantly expressed during rice seed maturation, we generated several transgenic rice plants in which various levels of BiP1 protein accumulated in an endosperm-specific manner. Severe suppression (BiP1 KD) or significant over-expression (BiP1 OEmax) of BiP1 not only altered seed phenotype and the intracellular structure of endosperm cells, but also reduced seed storage protein content, starch accumulation and grain weight. Microarray and RT-PCR analyses indicated that expression of many chaperone and co-chaperone genes was induced in transgenic plants, with more prominent expression in the BiP1 KD line than in the BiP1 OEmax line. Transcriptional induction of most chaperones was observed in calli treated with dithiothreitol or tunicamycin, treatments that trigger ER stress, indicating that induction of the chaperone genes in transgenic rice was caused by an ER stress response. In transient assays using rice protoplasts, the ortholog (Os06g0622700) of the AtbZIP60 transcription factor was shown to be involved in activation of some chaperone genes. Slight increases in the BiP1 level compared with wild-type, accompanied by increased levels of calnexin and protein disulfide isomerase-like proteins, resulted in significant enhancement of seed storage protein content, without any change in intracellular structure or seed phenotype. Judicious modification of BiP1 levels in transgenic rice can provide suitable conditions for the production of secretory proteins by alleviating ER stress. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/21223397/Expression_of_ER_quality_control_related_genes_in_response_to_changes_in_BiP1_levels_in_developing_rice_endosperm_ DB - PRIME DP - Unbound Medicine ER -