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Application of experimental design in optimization of the separation condition for determination of four active components in cold medicines by flow injection-capillary electrophoresis.
J Chromatogr Sci. 2011 Feb; 49(2):142-7.JC

Abstract

Orthogonal design (OD) was employed to optimize the separation condition of flow injection-capillary electrophoresis (FI-CE). In order to compare the optimum condition, uniform design and univariate approach were also adopted. The influences of variables such as buffer pH, buffer concentration, acetonitrile (ACN) percentage, and separation voltage were discussed. The optimum separation condition was established. The limits of detection were 1.94 × 10(-2), 6.40 × 10(-3), 1.16 × 10(-2) and 1.94 × 10(-2) μg/mL for dextromethorphan hydrobromide (Dex), chlorphenamine hydrogen maleate (Chl), pseudoephedrine hydrochloride (Pse), and paracetamol (Par), respectively. The RSDs of peaks areas were less than 2.0%. The results showed the OD was an effective method among experimental designs for optimizing the separation conditions of CE. The optimum condition was used for separation and determination of Dex, Chl, Pse, and Par in cold medicines. The average recovery was between 96.68-101.25%.

Authors+Show Affiliations

School of Pharmacy, Shandong University, Jinan 250012, China.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21223640

Citation

Liu, Xiumei, and Xingguo Chen. "Application of Experimental Design in Optimization of the Separation Condition for Determination of Four Active Components in Cold Medicines By Flow Injection-capillary Electrophoresis." Journal of Chromatographic Science, vol. 49, no. 2, 2011, pp. 142-7.
Liu X, Chen X. Application of experimental design in optimization of the separation condition for determination of four active components in cold medicines by flow injection-capillary electrophoresis. J Chromatogr Sci. 2011;49(2):142-7.
Liu, X., & Chen, X. (2011). Application of experimental design in optimization of the separation condition for determination of four active components in cold medicines by flow injection-capillary electrophoresis. Journal of Chromatographic Science, 49(2), 142-7.
Liu X, Chen X. Application of Experimental Design in Optimization of the Separation Condition for Determination of Four Active Components in Cold Medicines By Flow Injection-capillary Electrophoresis. J Chromatogr Sci. 2011;49(2):142-7. PubMed PMID: 21223640.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of experimental design in optimization of the separation condition for determination of four active components in cold medicines by flow injection-capillary electrophoresis. AU - Liu,Xiumei, AU - Chen,Xingguo, PY - 2011/1/13/entrez PY - 2011/1/13/pubmed PY - 2011/4/19/medline SP - 142 EP - 7 JF - Journal of chromatographic science JO - J Chromatogr Sci VL - 49 IS - 2 N2 - Orthogonal design (OD) was employed to optimize the separation condition of flow injection-capillary electrophoresis (FI-CE). In order to compare the optimum condition, uniform design and univariate approach were also adopted. The influences of variables such as buffer pH, buffer concentration, acetonitrile (ACN) percentage, and separation voltage were discussed. The optimum separation condition was established. The limits of detection were 1.94 × 10(-2), 6.40 × 10(-3), 1.16 × 10(-2) and 1.94 × 10(-2) μg/mL for dextromethorphan hydrobromide (Dex), chlorphenamine hydrogen maleate (Chl), pseudoephedrine hydrochloride (Pse), and paracetamol (Par), respectively. The RSDs of peaks areas were less than 2.0%. The results showed the OD was an effective method among experimental designs for optimizing the separation conditions of CE. The optimum condition was used for separation and determination of Dex, Chl, Pse, and Par in cold medicines. The average recovery was between 96.68-101.25%. SN - 1945-239X UR - https://www.unboundmedicine.com/medline/citation/21223640/Application_of_experimental_design_in_optimization_of_the_separation_condition_for_determination_of_four_active_components_in_cold_medicines_by_flow_injection_capillary_electrophoresis_ L2 - https://academic.oup.com/chromsci/article-lookup/doi/10.1093/chrsci/49.2.142 DB - PRIME DP - Unbound Medicine ER -