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Changes in spinal cord expression of fractalkine and its receptor in a rat model of disc herniation by autologous nucleus pulposus.
Spine (Phila Pa 1976). 2011 May 20; 36(12):E753-60.S

Abstract

STUDY DESIGN

Behavior, mRNA and immunohistochemical assessment of the expression of fractalkine (CX3CL1) and its receptor (CX3CR1) in a rat model of disc herniation by autologous nucleus pulposus (NP) implantation.

OBJECTIVE

To evaluate the changes in expression of fractalkine and its receptor in the spinal cord and their association with pain behavior in a rat model of disc herniation.

SUMMARY OF BACKGROUND DATA

Chemokines have recently been implicated in the pathophysiology of neuropathic pain. They mediate astrocytic migration and microglial proliferation, which are involved in the regulation of nociceptive transmission. Fractalkine is a chemokine, which participates in the mechanisms of neuropathic pain as a mediator of neuron-glia interactions.

METHODS

Sixty-six rats (NP-treated = 47, sham = 19) were implanted with autologous NP (approximately 3 mg) on the left L5 nerve root, just proximal to the dorsal root ganglion and tested for thermal hyperalgesia and mechanical allodynia before surgery and on days 1, 5, 10, 20, and 30 after surgery. The changes of expression of fractalkine and its receptor in the spinal cord were studied using real time PCR and immunohistochemistry.

RESULTS

Rats developed ipsilateral mechanical allodynia at day 1 and bilateral thermal hyperalgesia at day 5 after surgery, and these changes in sensitivity persisted throughout the observation period. The expression of mRNA for fractalkine in the spinal cord was increased by day 5 and remained upregulated for the duration of the experiment. The immunostaining for fractalkine increased in neurons and astrocytes and that for the fractalkine receptor increased in microglia in the dorsal horn ipsilateral to the disc herniation.

CONCLUSION

Our results indicate that autologous implantation of NP induces thermal hyperalgesia and mechanical allodynia, and leads to an upregulation of fractalkine and its receptor in spinal neurons and glia, implicating fractalkine in association with radicular pain.

Authors+Show Affiliations

Department of Rehabilitation Medicine, School of Medicine, Catholic University of Daegu, Daegu, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21224760

Citation

Park, Hea-Woon, et al. "Changes in Spinal Cord Expression of Fractalkine and Its Receptor in a Rat Model of Disc Herniation By Autologous Nucleus Pulposus." Spine, vol. 36, no. 12, 2011, pp. E753-60.
Park HW, Ahn SH, Kim SJ, et al. Changes in spinal cord expression of fractalkine and its receptor in a rat model of disc herniation by autologous nucleus pulposus. Spine (Phila Pa 1976). 2011;36(12):E753-60.
Park, H. W., Ahn, S. H., Kim, S. J., Seo, J. M., Cho, Y. W., Jang, S. H., Hwang, S. J., & Kwak, S. Y. (2011). Changes in spinal cord expression of fractalkine and its receptor in a rat model of disc herniation by autologous nucleus pulposus. Spine, 36(12), E753-60. https://doi.org/10.1097/BRS.0b013e3181ef610b
Park HW, et al. Changes in Spinal Cord Expression of Fractalkine and Its Receptor in a Rat Model of Disc Herniation By Autologous Nucleus Pulposus. Spine (Phila Pa 1976). 2011 May 20;36(12):E753-60. PubMed PMID: 21224760.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in spinal cord expression of fractalkine and its receptor in a rat model of disc herniation by autologous nucleus pulposus. AU - Park,Hea-Woon, AU - Ahn,Sang-Ho, AU - Kim,Su-Jeong, AU - Seo,Jeong-Min, AU - Cho,Yun-Woo, AU - Jang,Sung-Ho, AU - Hwang,Se-Jin, AU - Kwak,So-Young, PY - 2011/1/13/entrez PY - 2011/1/13/pubmed PY - 2012/3/30/medline SP - E753 EP - 60 JF - Spine JO - Spine (Phila Pa 1976) VL - 36 IS - 12 N2 - STUDY DESIGN: Behavior, mRNA and immunohistochemical assessment of the expression of fractalkine (CX3CL1) and its receptor (CX3CR1) in a rat model of disc herniation by autologous nucleus pulposus (NP) implantation. OBJECTIVE: To evaluate the changes in expression of fractalkine and its receptor in the spinal cord and their association with pain behavior in a rat model of disc herniation. SUMMARY OF BACKGROUND DATA: Chemokines have recently been implicated in the pathophysiology of neuropathic pain. They mediate astrocytic migration and microglial proliferation, which are involved in the regulation of nociceptive transmission. Fractalkine is a chemokine, which participates in the mechanisms of neuropathic pain as a mediator of neuron-glia interactions. METHODS: Sixty-six rats (NP-treated = 47, sham = 19) were implanted with autologous NP (approximately 3 mg) on the left L5 nerve root, just proximal to the dorsal root ganglion and tested for thermal hyperalgesia and mechanical allodynia before surgery and on days 1, 5, 10, 20, and 30 after surgery. The changes of expression of fractalkine and its receptor in the spinal cord were studied using real time PCR and immunohistochemistry. RESULTS: Rats developed ipsilateral mechanical allodynia at day 1 and bilateral thermal hyperalgesia at day 5 after surgery, and these changes in sensitivity persisted throughout the observation period. The expression of mRNA for fractalkine in the spinal cord was increased by day 5 and remained upregulated for the duration of the experiment. The immunostaining for fractalkine increased in neurons and astrocytes and that for the fractalkine receptor increased in microglia in the dorsal horn ipsilateral to the disc herniation. CONCLUSION: Our results indicate that autologous implantation of NP induces thermal hyperalgesia and mechanical allodynia, and leads to an upregulation of fractalkine and its receptor in spinal neurons and glia, implicating fractalkine in association with radicular pain. SN - 1528-1159 UR - https://www.unboundmedicine.com/medline/citation/21224760/Changes_in_spinal_cord_expression_of_fractalkine_and_its_receptor_in_a_rat_model_of_disc_herniation_by_autologous_nucleus_pulposus_ L2 - https://doi.org/10.1097/BRS.0b013e3181ef610b DB - PRIME DP - Unbound Medicine ER -