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A rapid, simple and sensitive liquid chromatography-tandem mass spectrometry method for routine clinical monitoring of tacrolimus with the Waters Masstrak™ immmunosuppressant kit.
Methods Find Exp Clin Pharmacol. 2010 Dec; 32(10):737-43.MF

Abstract

Advances in mass spectrometry instruments have led to increased utilization of high performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and it would be necessary to standardize blood quantification of immunosuppressant drugs. The aim of the study was to validate and assess the robustness of an LC-MS/MS method for quantification of tacrolimus in whole blood using the Waters Masstrak™ Immunosuppressant Kit. After protein precipitation from whole blood samples, chromatographic separation was performed in 2 min. Detection was performed with a Waters Tandem Quadrupole MS Quattro Premier XE, operated in multiple-reaction monitoring in positive electrospray ionization mode. This method was validated and compared to Enzyme Multiplied Immunoassay Technique (EMIT) method in accordance with actual guidelines. The limit of quantification was 1.0 ng/mL and the calibration curve was linear to 27.6 ng/mL. Between-day and within-day trueness and precision were < 15% at three concentrations spanning the linear range. The EMIT assay showed an average positive bias of 28.3% compared with the LC-MS/MS. Internal and external quality control were always accepted and demonstrated the robustness of this method. In conclusion, we validated a rapid, simple and robust quantification of tacrolimus in blood samples with the Waters Masstrak™ Immunosuppressant Kit.

Authors+Show Affiliations

Laboratoire de Pharmacocinétique et Toxicologie, CHU Timone, Marseille, France. romain.guilhaumou@ap-hm.frNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Validation Study

Language

eng

PubMed ID

21225010

Citation

Guilhaumou, R, et al. "A Rapid, Simple and Sensitive Liquid Chromatography-tandem Mass Spectrometry Method for Routine Clinical Monitoring of Tacrolimus With the Waters Masstrak™ Immmunosuppressant Kit." Methods and Findings in Experimental and Clinical Pharmacology, vol. 32, no. 10, 2010, pp. 737-43.
Guilhaumou R, Lacarelle B, Sampol-Manos E. A rapid, simple and sensitive liquid chromatography-tandem mass spectrometry method for routine clinical monitoring of tacrolimus with the Waters Masstrak™ immmunosuppressant kit. Methods Find Exp Clin Pharmacol. 2010;32(10):737-43.
Guilhaumou, R., Lacarelle, B., & Sampol-Manos, E. (2010). A rapid, simple and sensitive liquid chromatography-tandem mass spectrometry method for routine clinical monitoring of tacrolimus with the Waters Masstrak™ immmunosuppressant kit. Methods and Findings in Experimental and Clinical Pharmacology, 32(10), 737-43. https://doi.org/10.1358/mf.2010.32.10.1549035
Guilhaumou R, Lacarelle B, Sampol-Manos E. A Rapid, Simple and Sensitive Liquid Chromatography-tandem Mass Spectrometry Method for Routine Clinical Monitoring of Tacrolimus With the Waters Masstrak™ Immmunosuppressant Kit. Methods Find Exp Clin Pharmacol. 2010;32(10):737-43. PubMed PMID: 21225010.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A rapid, simple and sensitive liquid chromatography-tandem mass spectrometry method for routine clinical monitoring of tacrolimus with the Waters Masstrak™ immmunosuppressant kit. AU - Guilhaumou,R, AU - Lacarelle,B, AU - Sampol-Manos,E, PY - 2011/1/13/entrez PY - 2011/1/13/pubmed PY - 2011/5/13/medline SP - 737 EP - 43 JF - Methods and findings in experimental and clinical pharmacology JO - Methods Find Exp Clin Pharmacol VL - 32 IS - 10 N2 - Advances in mass spectrometry instruments have led to increased utilization of high performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and it would be necessary to standardize blood quantification of immunosuppressant drugs. The aim of the study was to validate and assess the robustness of an LC-MS/MS method for quantification of tacrolimus in whole blood using the Waters Masstrak™ Immunosuppressant Kit. After protein precipitation from whole blood samples, chromatographic separation was performed in 2 min. Detection was performed with a Waters Tandem Quadrupole MS Quattro Premier XE, operated in multiple-reaction monitoring in positive electrospray ionization mode. This method was validated and compared to Enzyme Multiplied Immunoassay Technique (EMIT) method in accordance with actual guidelines. The limit of quantification was 1.0 ng/mL and the calibration curve was linear to 27.6 ng/mL. Between-day and within-day trueness and precision were < 15% at three concentrations spanning the linear range. The EMIT assay showed an average positive bias of 28.3% compared with the LC-MS/MS. Internal and external quality control were always accepted and demonstrated the robustness of this method. In conclusion, we validated a rapid, simple and robust quantification of tacrolimus in blood samples with the Waters Masstrak™ Immunosuppressant Kit. SN - 0379-0355 UR - https://www.unboundmedicine.com/medline/citation/21225010/A_rapid_simple_and_sensitive_liquid_chromatography_tandem_mass_spectrometry_method_for_routine_clinical_monitoring_of_tacrolimus_with_the_Waters_Masstrak™_immmunosuppressant_kit_ L2 - http://journals.prous.com/journals/servlet/xmlxsl/pk_journals.xml_summaryn_pr?p_JournalId=6&amp;p_RefId=1549035 DB - PRIME DP - Unbound Medicine ER -