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Development of a mixed-mode solid phase extraction method and further gas chromatography mass spectrometry for the analysis of 3-alkyl-2-methoxypyrazines in wine.
J Chromatogr A. 2011 Feb 11; 1218(6):842-8.JC

Abstract

A new method for analysing 3-isopropyl-2-methoxypyrazine, 3-sec-butyl-2-methoxypyrazine and 3-isobutyl-2-methoxypyrazine in wine has been developed and applied to wine. The analytes are extracted from 25 mL of wine in a solid-phase extraction cartridge filled with 60 mg of cation-exchange mixed-mode sorbent. Analytes are recovered with triethylamine in dichloromethane and the organic extract is analysed by GC-SIM-MS using 3-isopropyl-2-ethoxypyrazine as internal standard. The detection limits of the method are in all cases under 1 ng/L, below the olfactory thresholds of the compounds in wine. The repeatability of the method is around 15% for levels in wine of 2 ng/L. Linearity is satisfactory and recoveries are in all cases close to 100% with RSD between 13% and 20%. The method has been applied to the analysis of 12 Chilean white and 8 Spanish red wines. The levels found suggest that 3-alkyl-2-methoxypyrazines can exert a significant sensory contribution to the aroma of Chilean Sauvignon Blanc wines, while most likely they play a nearly negligible role on traditional Ribera and Rioja Spanish red wines.

Authors+Show Affiliations

Departamento de Química Analítica, Facultad de Ciencias, Instituto de Investigación en Ingeniería de Aragón I3A, Universidad de Zaragoza, C/Pedro Cerbuna 12, 50009 Zaragoza, Spain. riclopez@unizar.esNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21227432

Citation

López, Ricardo, et al. "Development of a Mixed-mode Solid Phase Extraction Method and Further Gas Chromatography Mass Spectrometry for the Analysis of 3-alkyl-2-methoxypyrazines in Wine." Journal of Chromatography. A, vol. 1218, no. 6, 2011, pp. 842-8.
López R, Gracia-Moreno E, Cacho J, et al. Development of a mixed-mode solid phase extraction method and further gas chromatography mass spectrometry for the analysis of 3-alkyl-2-methoxypyrazines in wine. J Chromatogr A. 2011;1218(6):842-8.
López, R., Gracia-Moreno, E., Cacho, J., & Ferrreira, V. (2011). Development of a mixed-mode solid phase extraction method and further gas chromatography mass spectrometry for the analysis of 3-alkyl-2-methoxypyrazines in wine. Journal of Chromatography. A, 1218(6), 842-8. https://doi.org/10.1016/j.chroma.2010.12.048
López R, et al. Development of a Mixed-mode Solid Phase Extraction Method and Further Gas Chromatography Mass Spectrometry for the Analysis of 3-alkyl-2-methoxypyrazines in Wine. J Chromatogr A. 2011 Feb 11;1218(6):842-8. PubMed PMID: 21227432.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a mixed-mode solid phase extraction method and further gas chromatography mass spectrometry for the analysis of 3-alkyl-2-methoxypyrazines in wine. AU - López,Ricardo, AU - Gracia-Moreno,Elisa, AU - Cacho,Juan, AU - Ferrreira,Vicente, Y1 - 2010/12/21/ PY - 2010/07/19/received PY - 2010/11/25/revised PY - 2010/12/13/accepted PY - 2011/1/14/entrez PY - 2011/1/14/pubmed PY - 2011/5/11/medline SP - 842 EP - 8 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1218 IS - 6 N2 - A new method for analysing 3-isopropyl-2-methoxypyrazine, 3-sec-butyl-2-methoxypyrazine and 3-isobutyl-2-methoxypyrazine in wine has been developed and applied to wine. The analytes are extracted from 25 mL of wine in a solid-phase extraction cartridge filled with 60 mg of cation-exchange mixed-mode sorbent. Analytes are recovered with triethylamine in dichloromethane and the organic extract is analysed by GC-SIM-MS using 3-isopropyl-2-ethoxypyrazine as internal standard. The detection limits of the method are in all cases under 1 ng/L, below the olfactory thresholds of the compounds in wine. The repeatability of the method is around 15% for levels in wine of 2 ng/L. Linearity is satisfactory and recoveries are in all cases close to 100% with RSD between 13% and 20%. The method has been applied to the analysis of 12 Chilean white and 8 Spanish red wines. The levels found suggest that 3-alkyl-2-methoxypyrazines can exert a significant sensory contribution to the aroma of Chilean Sauvignon Blanc wines, while most likely they play a nearly negligible role on traditional Ribera and Rioja Spanish red wines. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/21227432/Development_of_a_mixed_mode_solid_phase_extraction_method_and_further_gas_chromatography_mass_spectrometry_for_the_analysis_of_3_alkyl_2_methoxypyrazines_in_wine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(10)01740-1 DB - PRIME DP - Unbound Medicine ER -