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Glutathione peroxidase3 of Saccharomyces cerevisiae protects phospholipids during cadmium-induced oxidative stress.
Antonie Van Leeuwenhoek. 2011 May; 99(4):761-71.AV

Abstract

The present study was undertaken to determine the role of glutathione peroxidase3 (gpx3) in phospholipid protection in cells. Wild-type (WT) cells showed an overall increase in phospholipids upon 50 μM cadmium (Cd)-treatment, whereas an untreated gpx3Δ strain showed a drastic reduction in overall phospholipids which was further reduced with 50 μM Cd. In WT cells, Cd-exposure increased the short chain fatty acids and decreased the unsaturated fatty acids and the magnitude was high in Cd-treated gpx3Δ cells. Purified recombinant gpx3p showed higher activity with phospholipid hydroperoxides than shorter hydroperoxides. An increase in gpx activity was observed in Cd-treated WT cells and no such alteration was observed in gpx3Δ. WT cells treated with Cd showed an increase in MDA over untreated, while untreated gpx3Δ cells themselves showed a higher level of MDA which was further enhanced with Cd-treatment. Iron, zinc and calcium levels were significantly altered in WT and gpx3Δ cells during Cd-treatment.

Authors+Show Affiliations

Department of Biochemistry, Bharathidasan University, Tiruchirappalli, 620 024, Tamilnadu, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21229313

Citation

Muthukumar, Kannan, et al. "Glutathione Peroxidase3 of Saccharomyces Cerevisiae Protects Phospholipids During Cadmium-induced Oxidative Stress." Antonie Van Leeuwenhoek, vol. 99, no. 4, 2011, pp. 761-71.
Muthukumar K, Rajakumar S, Sarkar MN, et al. Glutathione peroxidase3 of Saccharomyces cerevisiae protects phospholipids during cadmium-induced oxidative stress. Antonie Van Leeuwenhoek. 2011;99(4):761-71.
Muthukumar, K., Rajakumar, S., Sarkar, M. N., & Nachiappan, V. (2011). Glutathione peroxidase3 of Saccharomyces cerevisiae protects phospholipids during cadmium-induced oxidative stress. Antonie Van Leeuwenhoek, 99(4), 761-71. https://doi.org/10.1007/s10482-011-9550-9
Muthukumar K, et al. Glutathione Peroxidase3 of Saccharomyces Cerevisiae Protects Phospholipids During Cadmium-induced Oxidative Stress. Antonie Van Leeuwenhoek. 2011;99(4):761-71. PubMed PMID: 21229313.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glutathione peroxidase3 of Saccharomyces cerevisiae protects phospholipids during cadmium-induced oxidative stress. AU - Muthukumar,Kannan, AU - Rajakumar,Selvaraj, AU - Sarkar,Mary Nirmala, AU - Nachiappan,Vasanthi, Y1 - 2011/01/13/ PY - 2010/10/22/received PY - 2011/01/04/accepted PY - 2011/1/14/entrez PY - 2011/1/14/pubmed PY - 2011/10/26/medline SP - 761 EP - 71 JF - Antonie van Leeuwenhoek JO - Antonie Van Leeuwenhoek VL - 99 IS - 4 N2 - The present study was undertaken to determine the role of glutathione peroxidase3 (gpx3) in phospholipid protection in cells. Wild-type (WT) cells showed an overall increase in phospholipids upon 50 μM cadmium (Cd)-treatment, whereas an untreated gpx3Δ strain showed a drastic reduction in overall phospholipids which was further reduced with 50 μM Cd. In WT cells, Cd-exposure increased the short chain fatty acids and decreased the unsaturated fatty acids and the magnitude was high in Cd-treated gpx3Δ cells. Purified recombinant gpx3p showed higher activity with phospholipid hydroperoxides than shorter hydroperoxides. An increase in gpx activity was observed in Cd-treated WT cells and no such alteration was observed in gpx3Δ. WT cells treated with Cd showed an increase in MDA over untreated, while untreated gpx3Δ cells themselves showed a higher level of MDA which was further enhanced with Cd-treatment. Iron, zinc and calcium levels were significantly altered in WT and gpx3Δ cells during Cd-treatment. SN - 1572-9699 UR - https://www.unboundmedicine.com/medline/citation/21229313/Glutathione_peroxidase3_of_Saccharomyces_cerevisiae_protects_phospholipids_during_cadmium_induced_oxidative_stress_ L2 - https://doi.org/10.1007/s10482-011-9550-9 DB - PRIME DP - Unbound Medicine ER -