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Pentose phosphate pathway flux analysis for glycopeptide antibiotic vancomycin production during glucose-limited cultivation of Amycolatopsis orientalis.
Prep Biochem Biotechnol. 2011; 41(1):94-105.PB

Abstract

In vivo pentose phosphate pathway (PPP) enzymes such as glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), and transaldolase (TAL) activities as well as ATP- and ADP-level variations of Amycolatopsis orientalis were investigated with respect to glucose concentration and incubation period. G6PDH, 6PGDH, and TAL activities of A. orientalis reached maximum levels at 48 hr for all glucose concentrations used, after which the levels began to decline. G6PDH, 6PGDH, and TAL activities showed positive correlation with the glucose concentration up to 15 g/L, while further increases had an opposite effect. Intracellular ATP level showed a positive correlation with glucose concentrations, while ADP level increased up to 15 g/L. ATP concentration of A. orientalis increased rapidly at 48 hr of incubation, as was the case also for G6PDH, 6PGDH, and TAL activities, although the incubation period corresponding to maximum values of ADP shifted to 60 hr. Production of the glycopeptide antibiotic vancomycin increased with the increases in glucose concentrations up to 15 g/L, by showing coherence in the rates of oxidative and nonoxidative parts of the PPP.

Authors+Show Affiliations

Faculty of Science, Department of Chemistry, Biochemistry Division, University of Dokuz Eylul, Buca, Izmir, Turkey. hulya.kayali@deu.edu.tr

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21229467

Citation

Ayar-Kayali, Hulya. "Pentose Phosphate Pathway Flux Analysis for Glycopeptide Antibiotic Vancomycin Production During Glucose-limited Cultivation of Amycolatopsis Orientalis." Preparative Biochemistry & Biotechnology, vol. 41, no. 1, 2011, pp. 94-105.
Ayar-Kayali H. Pentose phosphate pathway flux analysis for glycopeptide antibiotic vancomycin production during glucose-limited cultivation of Amycolatopsis orientalis. Prep Biochem Biotechnol. 2011;41(1):94-105.
Ayar-Kayali, H. (2011). Pentose phosphate pathway flux analysis for glycopeptide antibiotic vancomycin production during glucose-limited cultivation of Amycolatopsis orientalis. Preparative Biochemistry & Biotechnology, 41(1), 94-105. https://doi.org/10.1080/10826068.2010.535401
Ayar-Kayali H. Pentose Phosphate Pathway Flux Analysis for Glycopeptide Antibiotic Vancomycin Production During Glucose-limited Cultivation of Amycolatopsis Orientalis. Prep Biochem Biotechnol. 2011;41(1):94-105. PubMed PMID: 21229467.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pentose phosphate pathway flux analysis for glycopeptide antibiotic vancomycin production during glucose-limited cultivation of Amycolatopsis orientalis. A1 - Ayar-Kayali,Hulya, PY - 2011/1/14/entrez PY - 2011/1/14/pubmed PY - 2011/6/23/medline SP - 94 EP - 105 JF - Preparative biochemistry & biotechnology JO - Prep. Biochem. Biotechnol. VL - 41 IS - 1 N2 - In vivo pentose phosphate pathway (PPP) enzymes such as glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), and transaldolase (TAL) activities as well as ATP- and ADP-level variations of Amycolatopsis orientalis were investigated with respect to glucose concentration and incubation period. G6PDH, 6PGDH, and TAL activities of A. orientalis reached maximum levels at 48 hr for all glucose concentrations used, after which the levels began to decline. G6PDH, 6PGDH, and TAL activities showed positive correlation with the glucose concentration up to 15 g/L, while further increases had an opposite effect. Intracellular ATP level showed a positive correlation with glucose concentrations, while ADP level increased up to 15 g/L. ATP concentration of A. orientalis increased rapidly at 48 hr of incubation, as was the case also for G6PDH, 6PGDH, and TAL activities, although the incubation period corresponding to maximum values of ADP shifted to 60 hr. Production of the glycopeptide antibiotic vancomycin increased with the increases in glucose concentrations up to 15 g/L, by showing coherence in the rates of oxidative and nonoxidative parts of the PPP. SN - 1532-2297 UR - https://www.unboundmedicine.com/medline/citation/21229467/Pentose_phosphate_pathway_flux_analysis_for_glycopeptide_antibiotic_vancomycin_production_during_glucose_limited_cultivation_of_Amycolatopsis_orientalis_ L2 - http://www.tandfonline.com/doi/full/10.1080/10826068.2010.535401 DB - PRIME DP - Unbound Medicine ER -