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Differentiation of hematopoietic stem cells in irradiated mouse thymic lobes. Kinetics and phenotype of progeny.
J Immunol. 1990 Dec 01; 145(11):3661-8.JI

Abstract

To define cell populations which participate in the very early stages of T cell development in the mouse thymus, we enriched hematopoietic stem cells from mouse bone marrow and injected them into thymic lobes of irradiated Ly-5 congenic recipients. The progeny of the stem cells were identified and their phenotypes were determined by two-color flow cytometry for the expression of various cell surface differentiation Ag during the course of their subsequent intrathymic development. The majority of the differentiation which occurred in the first 10 days after intrathymic cell transfer was myeloid in nature; hence, this study demonstrates that the irradiated thymus is not strictly selective for T cell development. Further, the maximum rate of T cell development was observed after intrathymic injection of 200 stem cells. Donor-derived cells which did not express Ag characteristic of the myeloid lineage could be detected and their phenotypes could be determined by flow cytometry as early as 7 days after intrathymic injection. At this time, the cells were still very similar phenotypically to the bone marrow hematopoietic stem cells. Exceptions to this were the expression of stem cell Ag 2 and a decrease in the level of MHC class I Ag expression. After 9 days, the donor-derived cells expressed high levels of the Thy-1 Ag and proceeded to change in cell surface phenotype as differentiation continued. These cell phenotypes are described for the time frame ending 18 days after injection, when most donor-derived cells were phenotypically small CD4+ CD8+ (double-positive) thymocytes.

Authors+Show Affiliations

Walter and Eliza Hall Institute for Medical Research, Royal Melbourne Hospital, Parkville, Victoria, Australia.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2123223

Citation

Spangrude, G J., and R Scollay. "Differentiation of Hematopoietic Stem Cells in Irradiated Mouse Thymic Lobes. Kinetics and Phenotype of Progeny." Journal of Immunology (Baltimore, Md. : 1950), vol. 145, no. 11, 1990, pp. 3661-8.
Spangrude GJ, Scollay R. Differentiation of hematopoietic stem cells in irradiated mouse thymic lobes. Kinetics and phenotype of progeny. J Immunol. 1990;145(11):3661-8.
Spangrude, G. J., & Scollay, R. (1990). Differentiation of hematopoietic stem cells in irradiated mouse thymic lobes. Kinetics and phenotype of progeny. Journal of Immunology (Baltimore, Md. : 1950), 145(11), 3661-8.
Spangrude GJ, Scollay R. Differentiation of Hematopoietic Stem Cells in Irradiated Mouse Thymic Lobes. Kinetics and Phenotype of Progeny. J Immunol. 1990 Dec 1;145(11):3661-8. PubMed PMID: 2123223.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differentiation of hematopoietic stem cells in irradiated mouse thymic lobes. Kinetics and phenotype of progeny. AU - Spangrude,G J, AU - Scollay,R, PY - 1990/12/1/pubmed PY - 1990/12/1/medline PY - 1990/12/1/entrez SP - 3661 EP - 8 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 145 IS - 11 N2 - To define cell populations which participate in the very early stages of T cell development in the mouse thymus, we enriched hematopoietic stem cells from mouse bone marrow and injected them into thymic lobes of irradiated Ly-5 congenic recipients. The progeny of the stem cells were identified and their phenotypes were determined by two-color flow cytometry for the expression of various cell surface differentiation Ag during the course of their subsequent intrathymic development. The majority of the differentiation which occurred in the first 10 days after intrathymic cell transfer was myeloid in nature; hence, this study demonstrates that the irradiated thymus is not strictly selective for T cell development. Further, the maximum rate of T cell development was observed after intrathymic injection of 200 stem cells. Donor-derived cells which did not express Ag characteristic of the myeloid lineage could be detected and their phenotypes could be determined by flow cytometry as early as 7 days after intrathymic injection. At this time, the cells were still very similar phenotypically to the bone marrow hematopoietic stem cells. Exceptions to this were the expression of stem cell Ag 2 and a decrease in the level of MHC class I Ag expression. After 9 days, the donor-derived cells expressed high levels of the Thy-1 Ag and proceeded to change in cell surface phenotype as differentiation continued. These cell phenotypes are described for the time frame ending 18 days after injection, when most donor-derived cells were phenotypically small CD4+ CD8+ (double-positive) thymocytes. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2123223/Differentiation_of_hematopoietic_stem_cells_in_irradiated_mouse_thymic_lobes__Kinetics_and_phenotype_of_progeny_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2123223 DB - PRIME DP - Unbound Medicine ER -