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Identification and tissue distribution of odorant binding protein genes in the lucerne plant bug Adelphocoris lineolatus (Goeze).
Insect Biochem Mol Biol. 2011 Apr; 41(4):254-63.IB

Abstract

Insect odorant binding proteins (OBPs) are required for insect olfaction perception and play a key role in transporting hydrophobic semiochemicals across the sensillum lymph to the olfactory receptors (Ors). We constructed two high-quality cDNA libraries from the male and female antennae of the lucerne plant bug, Adelphocoris lineolatus (Goeze) (Hemiptera: Miridae), a hemipteran species in a large and economically important phylogenetic group of phytophagous insects. A total of 1538 male ESTs and 1576 female ESTs were sequenced and analyzed, which produced 2915 high-quality ESTs for further analysis. The 2915 ESTs were assembled to 1423 unigenes. Of the 1423 unigenes, 895 (63%) showed no significant similarity with any known GenBank entry. The most prevalent transcripts in the cDNA libraries are OBPs, chemosensory proteins (CSPs), protein takeout precursors, antennae-specific proteins. We identified 14 genes encoding 12 "classical OBPs" with only six conserved cysteines and 2 "Plus-C OBPs" with two additional conserved cysteines and a conserved proline immediately after the sixth cysteine. AlinOBP4 has a very high amino acid identity of 89% to LAP, a well studied OBP of the tarnished plant bug Lygus lineolaris. The expression profiles of the 14 OBPs in different tissues (antennae, heads, thoraxes, abdomens, legs and wings) were measured by real-time qPCR. The results revealed some OBP genes are highly and differentially expressed in male and female antennae, and four OBP genes have an extremely high transcript level in the legs. Of two Plus-C OBP genes, one (AlinOBP14) is highly expressed only in the heads and another (AlinOBP7) is expressed in all olfactory tissues with much lower transcript levels comparing to other OBPs. The possible physiological functions of these OBPs are discussed.

Authors+Show Affiliations

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21232599

Citation

Gu, Shao-Hua, et al. "Identification and Tissue Distribution of Odorant Binding Protein Genes in the Lucerne Plant Bug Adelphocoris Lineolatus (Goeze)." Insect Biochemistry and Molecular Biology, vol. 41, no. 4, 2011, pp. 254-63.
Gu SH, Wang SP, Zhang XY, et al. Identification and tissue distribution of odorant binding protein genes in the lucerne plant bug Adelphocoris lineolatus (Goeze). Insect Biochem Mol Biol. 2011;41(4):254-63.
Gu, S. H., Wang, S. P., Zhang, X. Y., Wu, K. M., Guo, Y. Y., Zhou, J. J., & Zhang, Y. J. (2011). Identification and tissue distribution of odorant binding protein genes in the lucerne plant bug Adelphocoris lineolatus (Goeze). Insect Biochemistry and Molecular Biology, 41(4), 254-63. https://doi.org/10.1016/j.ibmb.2011.01.002
Gu SH, et al. Identification and Tissue Distribution of Odorant Binding Protein Genes in the Lucerne Plant Bug Adelphocoris Lineolatus (Goeze). Insect Biochem Mol Biol. 2011;41(4):254-63. PubMed PMID: 21232599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and tissue distribution of odorant binding protein genes in the lucerne plant bug Adelphocoris lineolatus (Goeze). AU - Gu,Shao-Hua, AU - Wang,Shu-Ping, AU - Zhang,Xue-Ying, AU - Wu,Kong-Ming, AU - Guo,Yu-Yuan, AU - Zhou,Jing-Jiang, AU - Zhang,Yong-Jun, Y1 - 2011/01/11/ PY - 2010/04/24/received PY - 2010/12/23/revised PY - 2011/01/03/accepted PY - 2011/1/15/entrez PY - 2011/1/15/pubmed PY - 2011/6/15/medline SP - 254 EP - 63 JF - Insect biochemistry and molecular biology JO - Insect Biochem Mol Biol VL - 41 IS - 4 N2 - Insect odorant binding proteins (OBPs) are required for insect olfaction perception and play a key role in transporting hydrophobic semiochemicals across the sensillum lymph to the olfactory receptors (Ors). We constructed two high-quality cDNA libraries from the male and female antennae of the lucerne plant bug, Adelphocoris lineolatus (Goeze) (Hemiptera: Miridae), a hemipteran species in a large and economically important phylogenetic group of phytophagous insects. A total of 1538 male ESTs and 1576 female ESTs were sequenced and analyzed, which produced 2915 high-quality ESTs for further analysis. The 2915 ESTs were assembled to 1423 unigenes. Of the 1423 unigenes, 895 (63%) showed no significant similarity with any known GenBank entry. The most prevalent transcripts in the cDNA libraries are OBPs, chemosensory proteins (CSPs), protein takeout precursors, antennae-specific proteins. We identified 14 genes encoding 12 "classical OBPs" with only six conserved cysteines and 2 "Plus-C OBPs" with two additional conserved cysteines and a conserved proline immediately after the sixth cysteine. AlinOBP4 has a very high amino acid identity of 89% to LAP, a well studied OBP of the tarnished plant bug Lygus lineolaris. The expression profiles of the 14 OBPs in different tissues (antennae, heads, thoraxes, abdomens, legs and wings) were measured by real-time qPCR. The results revealed some OBP genes are highly and differentially expressed in male and female antennae, and four OBP genes have an extremely high transcript level in the legs. Of two Plus-C OBP genes, one (AlinOBP14) is highly expressed only in the heads and another (AlinOBP7) is expressed in all olfactory tissues with much lower transcript levels comparing to other OBPs. The possible physiological functions of these OBPs are discussed. SN - 1879-0240 UR - https://www.unboundmedicine.com/medline/citation/21232599/Identification_and_tissue_distribution_of_odorant_binding_protein_genes_in_the_lucerne_plant_bug_Adelphocoris_lineolatus__Goeze__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(11)00004-X DB - PRIME DP - Unbound Medicine ER -