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Post-exercise protein synthesis rates are only marginally higher in type I compared with type II muscle fibres following resistance-type exercise.
Eur J Appl Physiol. 2011 Aug; 111(8):1871-8.EJ

Abstract

We examined the effect of an acute bout of resistance exercise on fractional muscle protein synthesis rates in human type I and type II muscle fibres. After a standardised breakfast (31 ± 1 kJ kg(-1) body weight, consisting of 52 Energy% (En%) carbohydrate, 34 En% protein and 14 En% fat), 9 untrained men completed a lower-limb resistance exercise bout (8 sets of 10 repetitions leg press and leg extension at 70% 1RM). A primed, continuous infusion of L: -[ring-(13)C(6)]phenylalanine was combined with muscle biopsies collected from both legs immediately after exercise and after 6 h of post-exercise recovery. Single muscle fibres were dissected from freeze-dried biopsies and stained for ATPase activity with pre-incubation at a pH of 4.3. Type I and II fibres were separated under a light microscope and analysed for protein-bound L: -[ring-(13)C(6)]phenylalanine labelling. Baseline (post-exercise) L: -[ring-(13)C(6)]phenylalanine muscle tissue labelling, expressed as (∂(13)C/(12)C), averaged -32.09 ± 0.28, -32.53 ± 0.10 and -32.02 ± 0.16 in the type I and II muscle fibres and mixed muscle, respectively (P = 0.14). During post-exercise recovery, muscle protein synthesis rates were marginally (8 ± 2%) higher in the type I than type II muscle fibres, at 0.100 ± 0.005 versus 0.094 ± 0.005%/h, respectively (P < 0.05), whereby rates of mixed muscle protein were 0.091 ± 0.005%/h. Muscle protein synthesis rates following resistance-type exercise are only marginally higher in type I compared with type II muscle fibres.

Authors+Show Affiliations

Department of Human Movement Sciences, NUTRIM School for Nutrition, Toxicology and Metabolism, Maastricht University Medical Centre, PO Box 616, 6200 MD, Maastricht, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21234594

Citation

Koopman, René, et al. "Post-exercise Protein Synthesis Rates Are Only Marginally Higher in Type I Compared With Type II Muscle Fibres Following Resistance-type Exercise." European Journal of Applied Physiology, vol. 111, no. 8, 2011, pp. 1871-8.
Koopman R, Gleeson BG, Gijsen AP, et al. Post-exercise protein synthesis rates are only marginally higher in type I compared with type II muscle fibres following resistance-type exercise. Eur J Appl Physiol. 2011;111(8):1871-8.
Koopman, R., Gleeson, B. G., Gijsen, A. P., Groen, B., Senden, J. M., Rennie, M. J., & van Loon, L. J. (2011). Post-exercise protein synthesis rates are only marginally higher in type I compared with type II muscle fibres following resistance-type exercise. European Journal of Applied Physiology, 111(8), 1871-8. https://doi.org/10.1007/s00421-010-1808-9
Koopman R, et al. Post-exercise Protein Synthesis Rates Are Only Marginally Higher in Type I Compared With Type II Muscle Fibres Following Resistance-type Exercise. Eur J Appl Physiol. 2011;111(8):1871-8. PubMed PMID: 21234594.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Post-exercise protein synthesis rates are only marginally higher in type I compared with type II muscle fibres following resistance-type exercise. AU - Koopman,René, AU - Gleeson,Benjamin G, AU - Gijsen,Annemie P, AU - Groen,Bart, AU - Senden,Joan M G, AU - Rennie,Michael J, AU - van Loon,Luc J C, Y1 - 2011/01/14/ PY - 2010/09/20/received PY - 2010/12/23/accepted PY - 2011/1/15/entrez PY - 2011/1/15/pubmed PY - 2011/12/14/medline SP - 1871 EP - 8 JF - European journal of applied physiology JO - Eur J Appl Physiol VL - 111 IS - 8 N2 - We examined the effect of an acute bout of resistance exercise on fractional muscle protein synthesis rates in human type I and type II muscle fibres. After a standardised breakfast (31 ± 1 kJ kg(-1) body weight, consisting of 52 Energy% (En%) carbohydrate, 34 En% protein and 14 En% fat), 9 untrained men completed a lower-limb resistance exercise bout (8 sets of 10 repetitions leg press and leg extension at 70% 1RM). A primed, continuous infusion of L: -[ring-(13)C(6)]phenylalanine was combined with muscle biopsies collected from both legs immediately after exercise and after 6 h of post-exercise recovery. Single muscle fibres were dissected from freeze-dried biopsies and stained for ATPase activity with pre-incubation at a pH of 4.3. Type I and II fibres were separated under a light microscope and analysed for protein-bound L: -[ring-(13)C(6)]phenylalanine labelling. Baseline (post-exercise) L: -[ring-(13)C(6)]phenylalanine muscle tissue labelling, expressed as (∂(13)C/(12)C), averaged -32.09 ± 0.28, -32.53 ± 0.10 and -32.02 ± 0.16 in the type I and II muscle fibres and mixed muscle, respectively (P = 0.14). During post-exercise recovery, muscle protein synthesis rates were marginally (8 ± 2%) higher in the type I than type II muscle fibres, at 0.100 ± 0.005 versus 0.094 ± 0.005%/h, respectively (P < 0.05), whereby rates of mixed muscle protein were 0.091 ± 0.005%/h. Muscle protein synthesis rates following resistance-type exercise are only marginally higher in type I compared with type II muscle fibres. SN - 1439-6327 UR - https://www.unboundmedicine.com/medline/citation/21234594/Post_exercise_protein_synthesis_rates_are_only_marginally_higher_in_type_I_compared_with_type_II_muscle_fibres_following_resistance_type_exercise_ L2 - https://dx.doi.org/10.1007/s00421-010-1808-9 DB - PRIME DP - Unbound Medicine ER -