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Nasal immunization with plasmid DNA encoding P6 protein and immunostimulatory complexes elicits nontypeable Haemophilus influenzae-specific long-term mucosal immune responses in the nasopharynx.
Vaccine. 2011 Feb 24; 29(10):1881-90.V

Abstract

Nasal vaccination is an effective therapeutic regimen for preventing upper respiratory infection, while DNA vaccines represent a new approach for controlling infectious diseases. Here, we examined the efficacy of nasally administered DNA vaccine on upper respiratory infections. A DNA plasmid encoding the P6 outer membrane protein of nontypeable Haemophilus influenzae (NTHi) was constructed. Mice were immunized 3 times intranasally with the DNA plasmid and Matrix-M, an immunostimulatory complex adjuvant. P6-specific immune responses were examined using purified P6 protein. Nasal-associated lymphoid tissue (NALT) CD4(+) T cells were purified and incubated with feeder cells in the presence of P6, and the expression of cytokine mRNA was examined. In addition, NTHi challenges were performed and the level of NTHi was quantified in nasal washes. P6-specific nasal wash IgA and serum IgG were elevated following immunization with the DNA plasmid and Matrix-M. The number of specific IgA-producing cells increased in the nasal passages of the immunized mice. In addition to Th1 and Th2 cytokine expression, IL-17 was detected in P6-specific NALT CD4(+) T cells. Moreover, DNA vaccination enhanced bacterial clearance. These findings suggest that a successful DNA vaccination protocol has been developed for inducing in vivo immune responses against NTHi. Nasal vaccination with P6 DNA vaccine and Matrix-M might be a new effective regimen for the induction of specific protective immunity in the upper respiratory tract.

Authors+Show Affiliations

Department of Otolaryngology, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hazama-cho, Yufu, Oita 879-5593, Japan. satoruk@med.oita-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21237276

Citation

Kodama, Satoru, et al. "Nasal Immunization With Plasmid DNA Encoding P6 Protein and Immunostimulatory Complexes Elicits Nontypeable Haemophilus Influenzae-specific Long-term Mucosal Immune Responses in the Nasopharynx." Vaccine, vol. 29, no. 10, 2011, pp. 1881-90.
Kodama S, Hirano T, Noda K, et al. Nasal immunization with plasmid DNA encoding P6 protein and immunostimulatory complexes elicits nontypeable Haemophilus influenzae-specific long-term mucosal immune responses in the nasopharynx. Vaccine. 2011;29(10):1881-90.
Kodama, S., Hirano, T., Noda, K., Umemoto, S., & Suzuki, M. (2011). Nasal immunization with plasmid DNA encoding P6 protein and immunostimulatory complexes elicits nontypeable Haemophilus influenzae-specific long-term mucosal immune responses in the nasopharynx. Vaccine, 29(10), 1881-90. https://doi.org/10.1016/j.vaccine.2010.12.129
Kodama S, et al. Nasal Immunization With Plasmid DNA Encoding P6 Protein and Immunostimulatory Complexes Elicits Nontypeable Haemophilus Influenzae-specific Long-term Mucosal Immune Responses in the Nasopharynx. Vaccine. 2011 Feb 24;29(10):1881-90. PubMed PMID: 21237276.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nasal immunization with plasmid DNA encoding P6 protein and immunostimulatory complexes elicits nontypeable Haemophilus influenzae-specific long-term mucosal immune responses in the nasopharynx. AU - Kodama,Satoru, AU - Hirano,Takashi, AU - Noda,Kenji, AU - Umemoto,Shingo, AU - Suzuki,Masashi, Y1 - 2011/01/13/ PY - 2010/09/16/received PY - 2010/11/26/revised PY - 2010/12/30/accepted PY - 2011/1/18/entrez PY - 2011/1/18/pubmed PY - 2011/6/1/medline SP - 1881 EP - 90 JF - Vaccine JO - Vaccine VL - 29 IS - 10 N2 - Nasal vaccination is an effective therapeutic regimen for preventing upper respiratory infection, while DNA vaccines represent a new approach for controlling infectious diseases. Here, we examined the efficacy of nasally administered DNA vaccine on upper respiratory infections. A DNA plasmid encoding the P6 outer membrane protein of nontypeable Haemophilus influenzae (NTHi) was constructed. Mice were immunized 3 times intranasally with the DNA plasmid and Matrix-M, an immunostimulatory complex adjuvant. P6-specific immune responses were examined using purified P6 protein. Nasal-associated lymphoid tissue (NALT) CD4(+) T cells were purified and incubated with feeder cells in the presence of P6, and the expression of cytokine mRNA was examined. In addition, NTHi challenges were performed and the level of NTHi was quantified in nasal washes. P6-specific nasal wash IgA and serum IgG were elevated following immunization with the DNA plasmid and Matrix-M. The number of specific IgA-producing cells increased in the nasal passages of the immunized mice. In addition to Th1 and Th2 cytokine expression, IL-17 was detected in P6-specific NALT CD4(+) T cells. Moreover, DNA vaccination enhanced bacterial clearance. These findings suggest that a successful DNA vaccination protocol has been developed for inducing in vivo immune responses against NTHi. Nasal vaccination with P6 DNA vaccine and Matrix-M might be a new effective regimen for the induction of specific protective immunity in the upper respiratory tract. SN - 1873-2518 UR - https://www.unboundmedicine.com/medline/citation/21237276/Nasal_immunization_with_plasmid_DNA_encoding_P6_protein_and_immunostimulatory_complexes_elicits_nontypeable_Haemophilus_influenzae_specific_long_term_mucosal_immune_responses_in_the_nasopharynx_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(11)00010-7 DB - PRIME DP - Unbound Medicine ER -