Tags

Type your tag names separated by a space and hit enter

Tormentic acid, a triterpenoid saponin, isolated from Rosa rugosa, inhibited LPS-induced iNOS, COX-2, and TNF-α expression through inactivation of the nuclear factor-κb pathway in RAW 264.7 macrophages.
Int Immunopharmacol. 2011 Apr; 11(4):504-10.II

Abstract

We previously reported that extract of Rosa rugosa root and its active triterpenoids constituents exhibit anti-nociceptive and anti-inflammatory effects in animal models. However, little is known about the effects and the molecular mechanism of the 19α-hydroxyursane-type triterpenoids. Among the tested 19α-hydroxyursane-type triterpenoids (kaji-ichigoside F(1), rosamultin, euscaphic acid, tormentic acid (TA)), TA was found to most potently inhibit the production of nitric oxide (NO) in RAW 264.7 cells. We investigated the anti-inflammatory effects and its underlying molecular mechanisms of TA in lipopolysaccaride (LPS)-stimulated RAW 264.7 cells. TA dose-dependently reduced the productions of NO, prostaglandin E(2) (PGE(2)), and tumor necrosis factor-α (TNF-α) induced by LPS. In addition, TA significantly suppressed the LPS-induced expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-α at the mRNA and protein levels. Moreover, treatment with TA decreased LPS-induced DNA binding of nuclear factor-kappa B (NF-κB) and nuclear translocation of p65 and p50 subunits of NF-κB. Consistent with these findings, TA also suppressed the LPS-stimulated degradation and phosphorylation of inhibitor of kappa B-α (IκB-α). Taken together, these results suggest that the anti-inflammatory activity of TA is associated with the down-regulation of iNOS, COX-2, and TNF-α through the negative regulation of the NF-κB pathway in RAW 264.7 cells.

Authors+Show Affiliations

Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21237302

Citation

An, Hyo-Jin, et al. "Tormentic Acid, a Triterpenoid Saponin, Isolated From Rosa Rugosa, Inhibited LPS-induced iNOS, COX-2, and TNF-α Expression Through Inactivation of the Nuclear Factor-κb Pathway in RAW 264.7 Macrophages." International Immunopharmacology, vol. 11, no. 4, 2011, pp. 504-10.
An HJ, Kim IT, Park HJ, et al. Tormentic acid, a triterpenoid saponin, isolated from Rosa rugosa, inhibited LPS-induced iNOS, COX-2, and TNF-α expression through inactivation of the nuclear factor-κb pathway in RAW 264.7 macrophages. Int Immunopharmacol. 2011;11(4):504-10.
An, H. J., Kim, I. T., Park, H. J., Kim, H. M., Choi, J. H., & Lee, K. T. (2011). Tormentic acid, a triterpenoid saponin, isolated from Rosa rugosa, inhibited LPS-induced iNOS, COX-2, and TNF-α expression through inactivation of the nuclear factor-κb pathway in RAW 264.7 macrophages. International Immunopharmacology, 11(4), 504-10. https://doi.org/10.1016/j.intimp.2011.01.002
An HJ, et al. Tormentic Acid, a Triterpenoid Saponin, Isolated From Rosa Rugosa, Inhibited LPS-induced iNOS, COX-2, and TNF-α Expression Through Inactivation of the Nuclear Factor-κb Pathway in RAW 264.7 Macrophages. Int Immunopharmacol. 2011;11(4):504-10. PubMed PMID: 21237302.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tormentic acid, a triterpenoid saponin, isolated from Rosa rugosa, inhibited LPS-induced iNOS, COX-2, and TNF-α expression through inactivation of the nuclear factor-κb pathway in RAW 264.7 macrophages. AU - An,Hyo-Jin, AU - Kim,In-Tae, AU - Park,Hee-Juhn, AU - Kim,Hyung-Min, AU - Choi,Jung-Hye, AU - Lee,Kyung-Tae, Y1 - 2011/01/13/ PY - 2010/10/26/received PY - 2010/12/30/revised PY - 2011/01/04/accepted PY - 2011/1/18/entrez PY - 2011/1/18/pubmed PY - 2011/7/16/medline SP - 504 EP - 10 JF - International immunopharmacology JO - Int Immunopharmacol VL - 11 IS - 4 N2 - We previously reported that extract of Rosa rugosa root and its active triterpenoids constituents exhibit anti-nociceptive and anti-inflammatory effects in animal models. However, little is known about the effects and the molecular mechanism of the 19α-hydroxyursane-type triterpenoids. Among the tested 19α-hydroxyursane-type triterpenoids (kaji-ichigoside F(1), rosamultin, euscaphic acid, tormentic acid (TA)), TA was found to most potently inhibit the production of nitric oxide (NO) in RAW 264.7 cells. We investigated the anti-inflammatory effects and its underlying molecular mechanisms of TA in lipopolysaccaride (LPS)-stimulated RAW 264.7 cells. TA dose-dependently reduced the productions of NO, prostaglandin E(2) (PGE(2)), and tumor necrosis factor-α (TNF-α) induced by LPS. In addition, TA significantly suppressed the LPS-induced expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-α at the mRNA and protein levels. Moreover, treatment with TA decreased LPS-induced DNA binding of nuclear factor-kappa B (NF-κB) and nuclear translocation of p65 and p50 subunits of NF-κB. Consistent with these findings, TA also suppressed the LPS-stimulated degradation and phosphorylation of inhibitor of kappa B-α (IκB-α). Taken together, these results suggest that the anti-inflammatory activity of TA is associated with the down-regulation of iNOS, COX-2, and TNF-α through the negative regulation of the NF-κB pathway in RAW 264.7 cells. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/21237302/Tormentic_acid_a_triterpenoid_saponin_isolated_from_Rosa_rugosa_inhibited_LPS_induced_iNOS_COX_2_and_TNF_α_expression_through_inactivation_of_the_nuclear_factor_κb_pathway_in_RAW_264_7_macrophages_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(11)00007-5 DB - PRIME DP - Unbound Medicine ER -