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Amelioration of selenite toxicity and cataractogenesis in cultured rat lenses by Vitex negundo.
Graefes Arch Clin Exp Ophthalmol. 2011 May; 249(5):685-92.GA

Abstract

PURPOSE

Recent evidence suggests that oxidative stress plays a significant role in the development of cataract. The present study sought to evaluate the efficacy of flavonoid fraction of Vitex negundo (FVN) in preventing the toxicity induced by sodium selenite in vitro culture condition.

METHODS

Enucleated rat lenses were maintained in organ culture containing DMEM medium alone (G I), supplemented with 0.1mM sodium selenite (G II), sodium selenite + 20 μg/ml quercetin (G III) and sodium selenite + 50 μg/ml FVN (G IV). Treatment was from the second to fifth day, while selenite administration was done on the third day. After the experimental period, lenses were taken out and the activities of superoxide dismutase (SOD), catalase, Ca(2+) ATPase, levels of reduced glutathione (GSH), calcium, reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), and sulfhydryl content were studied.

RESULTS

Morphological examination revealed dense vacuolization and loss of cortical transparency in G II compared to control and treated group. The mean activities of the enzymes SOD, catalase and Ca(2+) ATPase, levels of GSH and sulfhydryl content were significantly reduced in lenses of G II compared to control. In addition, the mean levels of ROS, calcium and TBARS were elevated in G II compared to control. However, these changes were modulated by FVN treatment to further strengthen its protective role over selenite cataract.

CONCLUSION

These results suggest that FVN treatment prevented selenite toxicity and cataractogenesis by maintaining antioxidant status, calcium homeostasis, protecting sulfhydryl group, and decreasing oxidative stress in lens, which may be due to its protective effects.

Authors+Show Affiliations

Department of Biochemistry, University of Kerala, Kariavattom, Kerala, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21240520

Citation

Rooban, B N., et al. "Amelioration of Selenite Toxicity and Cataractogenesis in Cultured Rat Lenses By Vitex Negundo." Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, vol. 249, no. 5, 2011, pp. 685-92.
Rooban BN, Sasikala V, Sahasranamam V, et al. Amelioration of selenite toxicity and cataractogenesis in cultured rat lenses by Vitex negundo. Graefes Arch Clin Exp Ophthalmol. 2011;249(5):685-92.
Rooban, B. N., Sasikala, V., Sahasranamam, V., & Abraham, A. (2011). Amelioration of selenite toxicity and cataractogenesis in cultured rat lenses by Vitex negundo. Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, 249(5), 685-92. https://doi.org/10.1007/s00417-010-1598-0
Rooban BN, et al. Amelioration of Selenite Toxicity and Cataractogenesis in Cultured Rat Lenses By Vitex Negundo. Graefes Arch Clin Exp Ophthalmol. 2011;249(5):685-92. PubMed PMID: 21240520.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amelioration of selenite toxicity and cataractogenesis in cultured rat lenses by Vitex negundo. AU - Rooban,B N, AU - Sasikala,V, AU - Sahasranamam,V, AU - Abraham,Annie, Y1 - 2011/01/15/ PY - 2010/07/20/received PY - 2010/12/02/accepted PY - 2010/11/01/revised PY - 2011/1/18/entrez PY - 2011/1/18/pubmed PY - 2011/8/27/medline SP - 685 EP - 92 JF - Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie JO - Graefes Arch Clin Exp Ophthalmol VL - 249 IS - 5 N2 - PURPOSE: Recent evidence suggests that oxidative stress plays a significant role in the development of cataract. The present study sought to evaluate the efficacy of flavonoid fraction of Vitex negundo (FVN) in preventing the toxicity induced by sodium selenite in vitro culture condition. METHODS: Enucleated rat lenses were maintained in organ culture containing DMEM medium alone (G I), supplemented with 0.1mM sodium selenite (G II), sodium selenite + 20 μg/ml quercetin (G III) and sodium selenite + 50 μg/ml FVN (G IV). Treatment was from the second to fifth day, while selenite administration was done on the third day. After the experimental period, lenses were taken out and the activities of superoxide dismutase (SOD), catalase, Ca(2+) ATPase, levels of reduced glutathione (GSH), calcium, reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), and sulfhydryl content were studied. RESULTS: Morphological examination revealed dense vacuolization and loss of cortical transparency in G II compared to control and treated group. The mean activities of the enzymes SOD, catalase and Ca(2+) ATPase, levels of GSH and sulfhydryl content were significantly reduced in lenses of G II compared to control. In addition, the mean levels of ROS, calcium and TBARS were elevated in G II compared to control. However, these changes were modulated by FVN treatment to further strengthen its protective role over selenite cataract. CONCLUSION: These results suggest that FVN treatment prevented selenite toxicity and cataractogenesis by maintaining antioxidant status, calcium homeostasis, protecting sulfhydryl group, and decreasing oxidative stress in lens, which may be due to its protective effects. SN - 1435-702X UR - https://www.unboundmedicine.com/medline/citation/21240520/Amelioration_of_selenite_toxicity_and_cataractogenesis_in_cultured_rat_lenses_by_Vitex_negundo_ L2 - https://dx.doi.org/10.1007/s00417-010-1598-0 DB - PRIME DP - Unbound Medicine ER -