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Combined simulation and experimental study of large deformation of red blood cells in microfluidic systems.
Ann Biomed Eng 2011; 39(3):1041-50AB

Abstract

We investigate the biophysical characteristics of healthy human red blood cells (RBCs) traversing microfluidic channels with cross-sectional areas as small as 2.7 × 3 μm. We combine single RBC optical tweezers and flow experiments with corresponding simulations based on dissipative particle dynamics (DPD), and upon validation of the DPD model, predictive simulations and companion experiments are performed in order to quantify cell deformation and pressure-velocity relationships for different channel sizes and physiologically relevant temperatures. We discuss conditions associated with the shape transitions of RBCs along with the relative effects of membrane and cytosol viscosity, plasma environments, and geometry on flow through microfluidic systems at physiological temperatures. In particular, we identify a cross-sectional area threshold below which the RBC membrane properties begin to dominate its flow behavior at room temperature; at physiological temperatures this effect is less profound.

Authors+Show Affiliations

Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

21240637

Citation

Quinn, David J., et al. "Combined Simulation and Experimental Study of Large Deformation of Red Blood Cells in Microfluidic Systems." Annals of Biomedical Engineering, vol. 39, no. 3, 2011, pp. 1041-50.
Quinn DJ, Pivkin I, Wong SY, et al. Combined simulation and experimental study of large deformation of red blood cells in microfluidic systems. Ann Biomed Eng. 2011;39(3):1041-50.
Quinn, D. J., Pivkin, I., Wong, S. Y., Chiam, K. H., Dao, M., Karniadakis, G. E., & Suresh, S. (2011). Combined simulation and experimental study of large deformation of red blood cells in microfluidic systems. Annals of Biomedical Engineering, 39(3), pp. 1041-50. doi:10.1007/s10439-010-0232-y.
Quinn DJ, et al. Combined Simulation and Experimental Study of Large Deformation of Red Blood Cells in Microfluidic Systems. Ann Biomed Eng. 2011;39(3):1041-50. PubMed PMID: 21240637.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combined simulation and experimental study of large deformation of red blood cells in microfluidic systems. AU - Quinn,David J, AU - Pivkin,Igor, AU - Wong,Sophie Y, AU - Chiam,Keng-Hwee, AU - Dao,Ming, AU - Karniadakis,George Em, AU - Suresh,Subra, Y1 - 2010/12/14/ PY - 2010/09/02/received PY - 2010/12/01/accepted PY - 2011/1/18/entrez PY - 2011/1/18/pubmed PY - 2011/6/3/medline SP - 1041 EP - 50 JF - Annals of biomedical engineering JO - Ann Biomed Eng VL - 39 IS - 3 N2 - We investigate the biophysical characteristics of healthy human red blood cells (RBCs) traversing microfluidic channels with cross-sectional areas as small as 2.7 × 3 μm. We combine single RBC optical tweezers and flow experiments with corresponding simulations based on dissipative particle dynamics (DPD), and upon validation of the DPD model, predictive simulations and companion experiments are performed in order to quantify cell deformation and pressure-velocity relationships for different channel sizes and physiologically relevant temperatures. We discuss conditions associated with the shape transitions of RBCs along with the relative effects of membrane and cytosol viscosity, plasma environments, and geometry on flow through microfluidic systems at physiological temperatures. In particular, we identify a cross-sectional area threshold below which the RBC membrane properties begin to dominate its flow behavior at room temperature; at physiological temperatures this effect is less profound. SN - 1573-9686 UR - https://www.unboundmedicine.com/medline/citation/21240637/Combined_simulation_and_experimental_study_of_large_deformation_of_red_blood_cells_in_microfluidic_systems_ L2 - https://doi.org/10.1007/s10439-010-0232-y DB - PRIME DP - Unbound Medicine ER -