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Molecular epidemiology of Orientia tsutsugamushi in Cambodia and Central Vietnam reveals a broad region-wide genetic diversity.
Infect Genet Evol. 2013 Apr; 15:35-42.IG

Abstract

Scrub typhus is an acute infectious disease caused by an obligate intracellular bacterium Orientia tsutsugamushi following the bite of infected trombiculid mites of the genus Leptotrombidium. This zoonotic disease is a major cause of febrile illness in the Asia-Pacific region, with a large spectrum of clinical manifestations from unapparent or mild disease to fatal disease. O. tsutsugamushi is characterized by a very high genomic plasticity and a large number of antigenic variants amongst strains. The 56-kDa type specific antigen (TSA) gene, encoding the major antigenic protein, was used as reference to investigate the genetic relationships between the strains and to genotype O. tsutsugamushi isolates. The open reading frame of the 56-kDa TSA gene of 41 sequences (28 Cambodian and 13 Vietnamese strains) from patient samples were sequenced and used for genotyping. The 28 Cambodian isolates clustered into 5 major groups, including Karp (43.5%), JG-v (25%), Kato/TA716 (21.5%), TA763 (3.5%) and Gilliam (3.5%). Karp (77%), TA763 (15.5%) and JG-v (7.5%) strains were identified amongst the 13 Vietnamese isolates. This is the first countrywide genotyping description in Cambodia and in Central Vietnam. These results demonstrate the considerable diversity of genotypes in co-circulation in both countries. The genotyping result might raise awareness amongst Cambodian and Vietnamese clinicians of the high genetic diversity of circulating O. tsutsugamushi strains and provides unique and beneficial data for serological and molecular diagnosis of scrub typhus infections as well as raw materials for future studies and vaccine development.

Authors+Show Affiliations

Institut Pasteur in Cambodia, Virology Unit, 5 Monivong Blvd., Phnom Penh, Cambodia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21241829

Citation

Duong, Veasna, et al. "Molecular Epidemiology of Orientia Tsutsugamushi in Cambodia and Central Vietnam Reveals a Broad Region-wide Genetic Diversity." Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, vol. 15, 2013, pp. 35-42.
Duong V, Mai TT, Blasdell K, et al. Molecular epidemiology of Orientia tsutsugamushi in Cambodia and Central Vietnam reveals a broad region-wide genetic diversity. Infect Genet Evol. 2013;15:35-42.
Duong, V., Mai, T. T., Blasdell, K., Lo, l. e. . V., Morvan, C., Lay, S., Anukool, W., Wongprompitak, P., Suputtamongkol, Y., Laurent, D., Richner, B., Ra, C., Chien, B. T., Frutos, R., & Buchy, P. (2013). Molecular epidemiology of Orientia tsutsugamushi in Cambodia and Central Vietnam reveals a broad region-wide genetic diversity. Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 15, 35-42. https://doi.org/10.1016/j.meegid.2011.01.004
Duong V, et al. Molecular Epidemiology of Orientia Tsutsugamushi in Cambodia and Central Vietnam Reveals a Broad Region-wide Genetic Diversity. Infect Genet Evol. 2013;15:35-42. PubMed PMID: 21241829.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular epidemiology of Orientia tsutsugamushi in Cambodia and Central Vietnam reveals a broad region-wide genetic diversity. AU - Duong,Veasna, AU - Mai,Trinh Thi Xuan, AU - Blasdell,Kim, AU - Lo,Le Viet, AU - Morvan,Claire, AU - Lay,Sreyrath, AU - Anukool,Wichittra, AU - Wongprompitak,Patimaporn, AU - Suputtamongkol,Yupin, AU - Laurent,Denis, AU - Richner,Beat, AU - Ra,Chheang, AU - Chien,Bui Trong, AU - Frutos,Roger, AU - Buchy,Philippe, Y1 - 2011/01/15/ PY - 2010/10/23/received PY - 2010/12/23/revised PY - 2011/01/04/accepted PY - 2011/1/19/entrez PY - 2011/1/19/pubmed PY - 2014/1/23/medline SP - 35 EP - 42 JF - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JO - Infect Genet Evol VL - 15 N2 - Scrub typhus is an acute infectious disease caused by an obligate intracellular bacterium Orientia tsutsugamushi following the bite of infected trombiculid mites of the genus Leptotrombidium. This zoonotic disease is a major cause of febrile illness in the Asia-Pacific region, with a large spectrum of clinical manifestations from unapparent or mild disease to fatal disease. O. tsutsugamushi is characterized by a very high genomic plasticity and a large number of antigenic variants amongst strains. The 56-kDa type specific antigen (TSA) gene, encoding the major antigenic protein, was used as reference to investigate the genetic relationships between the strains and to genotype O. tsutsugamushi isolates. The open reading frame of the 56-kDa TSA gene of 41 sequences (28 Cambodian and 13 Vietnamese strains) from patient samples were sequenced and used for genotyping. The 28 Cambodian isolates clustered into 5 major groups, including Karp (43.5%), JG-v (25%), Kato/TA716 (21.5%), TA763 (3.5%) and Gilliam (3.5%). Karp (77%), TA763 (15.5%) and JG-v (7.5%) strains were identified amongst the 13 Vietnamese isolates. This is the first countrywide genotyping description in Cambodia and in Central Vietnam. These results demonstrate the considerable diversity of genotypes in co-circulation in both countries. The genotyping result might raise awareness amongst Cambodian and Vietnamese clinicians of the high genetic diversity of circulating O. tsutsugamushi strains and provides unique and beneficial data for serological and molecular diagnosis of scrub typhus infections as well as raw materials for future studies and vaccine development. SN - 1567-7257 UR - https://www.unboundmedicine.com/medline/citation/21241829/Molecular_epidemiology_of_Orientia_tsutsugamushi_in_Cambodia_and_Central_Vietnam_reveals_a_broad_region_wide_genetic_diversity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-1348(11)00007-4 DB - PRIME DP - Unbound Medicine ER -