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Reconstitution of CMV pp65 and IE-1-specific IFN-γ CD8(+) and CD4(+) T-cell responses affording protection from CMV DNAemia following allogeneic hematopoietic SCT.
Bone Marrow Transplant. 2011 Nov; 46(11):1437-43.BM

Abstract

Threshold levels of CMV-specific T-cell populations presumably affording protection from active CMV infection in allo-SCT recipients have been proposed, but lack extensive validation. We quantified CMV pp65 and immediate-early 1-specific IFN-γ CD8(+) and CD4(+) T cell responses at days +30, +60 and +90 after transplantation in 133 patients, and established cutoff cell levels protecting from CMV DNAemia within the first 120 days after transplantation. No patients showing IFN-γ CD8(+) or IFN-γ CD4(+) T-cell counts >1.0 and >1.2 cells/μL, respectively, developed a subsequent episode of CMV DNAemia. Initial or recurrent episodes of CMV DNAemia occurred in the face of IFN-γ T-cell levels below defined thresholds. Negative predictive values at day +30 for the IFN-γ CD8(+) and CD4(+) T-cell markers were 68.1 and 61.8%, respectively. Recipients of grafts from CMV seropositive, related or HLA-matched donors, or receiving non-myeloablative conditioning had nonsignificant tendencies to reach more frequently protective levels of both T-cell subsets at early and late (day +365) times after transplantation. The use of anti-thymocyte globulin and umbilical cord blood transplantation were associated with impaired CMV-specific T-cell reconstitution. CMV-specific IFN-γ CD8(+) and CD4(+) T-cell recovery occurred irrespective of detectable CMV DNAemia.

Authors+Show Affiliations

Microbiology Service, Department of Microbiology, Hospital Clínico Universitario, School of Medicine, Valencia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21243030

Citation

Tormo, N, et al. "Reconstitution of CMV Pp65 and IE-1-specific IFN-γ CD8(+) and CD4(+) T-cell Responses Affording Protection From CMV DNAemia Following Allogeneic Hematopoietic SCT." Bone Marrow Transplantation, vol. 46, no. 11, 2011, pp. 1437-43.
Tormo N, Solano C, Benet I, et al. Reconstitution of CMV pp65 and IE-1-specific IFN-γ CD8(+) and CD4(+) T-cell responses affording protection from CMV DNAemia following allogeneic hematopoietic SCT. Bone Marrow Transplant. 2011;46(11):1437-43.
Tormo, N., Solano, C., Benet, I., Nieto, J., de la Cámara, R., López, J., Garcia-Noblejas, A., Muñoz-Cobo, B., Costa, E., Clari, M. A., Hernández-Boluda, J. C., Remigia, M. J., & Navarro, D. (2011). Reconstitution of CMV pp65 and IE-1-specific IFN-γ CD8(+) and CD4(+) T-cell responses affording protection from CMV DNAemia following allogeneic hematopoietic SCT. Bone Marrow Transplantation, 46(11), 1437-43. https://doi.org/10.1038/bmt.2010.330
Tormo N, et al. Reconstitution of CMV Pp65 and IE-1-specific IFN-γ CD8(+) and CD4(+) T-cell Responses Affording Protection From CMV DNAemia Following Allogeneic Hematopoietic SCT. Bone Marrow Transplant. 2011;46(11):1437-43. PubMed PMID: 21243030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reconstitution of CMV pp65 and IE-1-specific IFN-γ CD8(+) and CD4(+) T-cell responses affording protection from CMV DNAemia following allogeneic hematopoietic SCT. AU - Tormo,N, AU - Solano,C, AU - Benet,I, AU - Nieto,J, AU - de la Cámara,R, AU - López,J, AU - Garcia-Noblejas,A, AU - Muñoz-Cobo,B, AU - Costa,E, AU - Clari,M A, AU - Hernández-Boluda,J C, AU - Remigia,M J, AU - Navarro,D, Y1 - 2011/01/17/ PY - 2011/1/19/entrez PY - 2011/1/19/pubmed PY - 2012/3/3/medline SP - 1437 EP - 43 JF - Bone marrow transplantation JO - Bone Marrow Transplant VL - 46 IS - 11 N2 - Threshold levels of CMV-specific T-cell populations presumably affording protection from active CMV infection in allo-SCT recipients have been proposed, but lack extensive validation. We quantified CMV pp65 and immediate-early 1-specific IFN-γ CD8(+) and CD4(+) T cell responses at days +30, +60 and +90 after transplantation in 133 patients, and established cutoff cell levels protecting from CMV DNAemia within the first 120 days after transplantation. No patients showing IFN-γ CD8(+) or IFN-γ CD4(+) T-cell counts >1.0 and >1.2 cells/μL, respectively, developed a subsequent episode of CMV DNAemia. Initial or recurrent episodes of CMV DNAemia occurred in the face of IFN-γ T-cell levels below defined thresholds. Negative predictive values at day +30 for the IFN-γ CD8(+) and CD4(+) T-cell markers were 68.1 and 61.8%, respectively. Recipients of grafts from CMV seropositive, related or HLA-matched donors, or receiving non-myeloablative conditioning had nonsignificant tendencies to reach more frequently protective levels of both T-cell subsets at early and late (day +365) times after transplantation. The use of anti-thymocyte globulin and umbilical cord blood transplantation were associated with impaired CMV-specific T-cell reconstitution. CMV-specific IFN-γ CD8(+) and CD4(+) T-cell recovery occurred irrespective of detectable CMV DNAemia. SN - 1476-5365 UR - https://www.unboundmedicine.com/medline/citation/21243030/Reconstitution_of_CMV_pp65_and_IE_1_specific_IFN_��_CD8_+__and_CD4_+__T_cell_responses_affording_protection_from_CMV_DNAemia_following_allogeneic_hematopoietic_SCT_ L2 - https://doi.org/10.1038/bmt.2010.330 DB - PRIME DP - Unbound Medicine ER -