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Protective role of diallyl tetrasulfide on cadmium-induced testicular damage in adult rats: a biochemical and histological study.

Abstract

Cadmium (Cd)-induced oxidative damage is the most serious problem that leads to reproductive system failure in both human and animals. Our previous studies indicate that diallyl tetrasulfide (DTS) from garlic has the cytoprotective and antioxidant activity against Cd-induced toxicity in vivo and in vitro. The present investigation was carried out to find the influence of DTS on peroxidative damage induced by Cd in rat testes. The Cd-exposed rat testis showed a significant (p < 0.05) decrease in testes to body weight ratio, along with a significant (p < 0.05) increase in Cd accumulation, lipid peroxidation and protein carbonyl levels. In Cd-exposed rats, we also observed a significant (p < 0.05) decrease in the activities of antioxidant (superoxide dismutase, catalase and glutathione peroxidase) and glutathione metabolizing (glutathione-S-transferase, glutathione reductase and glucose-6-phosphate dehydrogenase) enzymes as well as reduced levels of non-enzymic (reduced glutathione, ascorbate and total sulphydryl groups) antioxidants. In contrast, treatment with DTS (40 mg/kg body weight orally) significantly (p < 0.05) reduced the accumulation of Cd and lipid peroxidation markers and also significantly improved the activities of antioxidant defense system in testes. Testicular protection by DTS is further substantiated by remarkable reduction of Cd-induced pathological changes. Our study has revealed that DTS renders protection against Cd-induced testicular injury by reducing Cd-mediated oxidative damage.

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  • Authors+Show Affiliations

    ,

    Department of Medicine, Brown University School of Medicine, Rhode Island Hospital, Providence, RI 02903, USA. Ponnusamy_Murugavel@brown.edu

    Source

    Toxicology and industrial health 27:5 2011 Jun pg 407-16

    MeSH

    Allyl Compounds
    Animals
    Antioxidants
    Ascorbic Acid
    Cadmium
    Catalase
    Garlic
    Glucosephosphate Dehydrogenase
    Glutathione
    Glutathione Reductase
    Glutathione Transferase
    Lipid Peroxidation
    Male
    Oxidative Stress
    Plant Extracts
    Protein Carbonylation
    Rats
    Rats, Wistar
    Sulfides
    Superoxide Dismutase
    Testicular Diseases
    Testis

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    21245201

    Citation

    Ponnusamy, Murugavel, and Leelavinothan Pari. "Protective Role of Diallyl Tetrasulfide On Cadmium-induced Testicular Damage in Adult Rats: a Biochemical and Histological Study." Toxicology and Industrial Health, vol. 27, no. 5, 2011, pp. 407-16.
    Ponnusamy M, Pari L. Protective role of diallyl tetrasulfide on cadmium-induced testicular damage in adult rats: a biochemical and histological study. Toxicol Ind Health. 2011;27(5):407-16.
    Ponnusamy, M., & Pari, L. (2011). Protective role of diallyl tetrasulfide on cadmium-induced testicular damage in adult rats: a biochemical and histological study. Toxicology and Industrial Health, 27(5), pp. 407-16. doi:10.1177/0748233710387633.
    Ponnusamy M, Pari L. Protective Role of Diallyl Tetrasulfide On Cadmium-induced Testicular Damage in Adult Rats: a Biochemical and Histological Study. Toxicol Ind Health. 2011;27(5):407-16. PubMed PMID: 21245201.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Protective role of diallyl tetrasulfide on cadmium-induced testicular damage in adult rats: a biochemical and histological study. AU - Ponnusamy,Murugavel, AU - Pari,Leelavinothan, Y1 - 2011/01/18/ PY - 2011/1/20/entrez PY - 2011/1/20/pubmed PY - 2011/9/13/medline SP - 407 EP - 16 JF - Toxicology and industrial health JO - Toxicol Ind Health VL - 27 IS - 5 N2 - Cadmium (Cd)-induced oxidative damage is the most serious problem that leads to reproductive system failure in both human and animals. Our previous studies indicate that diallyl tetrasulfide (DTS) from garlic has the cytoprotective and antioxidant activity against Cd-induced toxicity in vivo and in vitro. The present investigation was carried out to find the influence of DTS on peroxidative damage induced by Cd in rat testes. The Cd-exposed rat testis showed a significant (p < 0.05) decrease in testes to body weight ratio, along with a significant (p < 0.05) increase in Cd accumulation, lipid peroxidation and protein carbonyl levels. In Cd-exposed rats, we also observed a significant (p < 0.05) decrease in the activities of antioxidant (superoxide dismutase, catalase and glutathione peroxidase) and glutathione metabolizing (glutathione-S-transferase, glutathione reductase and glucose-6-phosphate dehydrogenase) enzymes as well as reduced levels of non-enzymic (reduced glutathione, ascorbate and total sulphydryl groups) antioxidants. In contrast, treatment with DTS (40 mg/kg body weight orally) significantly (p < 0.05) reduced the accumulation of Cd and lipid peroxidation markers and also significantly improved the activities of antioxidant defense system in testes. Testicular protection by DTS is further substantiated by remarkable reduction of Cd-induced pathological changes. Our study has revealed that DTS renders protection against Cd-induced testicular injury by reducing Cd-mediated oxidative damage. SN - 1477-0393 UR - https://www.unboundmedicine.com/medline/citation/21245201/Protective_role_of_diallyl_tetrasulfide_on_cadmium_induced_testicular_damage_in_adult_rats:_a_biochemical_and_histological_study_ L2 - http://journals.sagepub.com/doi/full/10.1177/0748233710387633?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -