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Long-term in vivo imaging and measurement of dendritic shrinkage of retinal ganglion cells.
Invest Ophthalmol Vis Sci. 2011 Mar 01; 52(3):1539-47.IO

Abstract

PURPOSE

To monitor and measure dendritic shrinkage of retinal ganglion cells (RGCs) in a strain of transgenic mice (Thy-1 YFP) that expresses yellow fluorescent proteins in neurons under the control of a Thy-1 promoter.

METHODS

A total of 125 RGCs from 16 eyes of Thy-1 YFP transgenic mice were serially imaged with a confocal scanning laser ophthalmoscope for 6 months after optic nerve crush. Quantitative analysis of cell body area, axon diameter, dendritic field, number of terminal branches, total dendritic branch length, branching complexity, symmetry, and distance from the optic disc was used to characterize the morphology of RGCs, describe the patterns of axonal and dendritic degeneration, identify the morphologic predictors for cell survival, and estimate the rate of dendritic shrinkage.

RESULTS

RGC damage was observed prospectively to begin with progressive dendritic shrinkage, followed by loss of the axon and the cell body. In a small proportion of RGCs, progressive axonal changes including fragmentation, beading, retraction, and bulb formation were also observed. RGCs with a larger dendritic field and a longer total dendritic branch length in general have a better survival probability. The rate of dendritic shrinkage was variable with a slower rate observed in cells having a larger dendritic field, a longer total dendritic branch length, and a greater distance from the optic disc.

CONCLUSIONS

Estimating the probability of RGC survival and measuring the rate of dendritic shrinkage could become a new paradigm for investigating neuronal degeneration and evaluating the response of neuroprotective treatment.

Authors+Show Affiliations

Department of Ophthalmology and Visual Sciences, Chinese University of Hong Kong, Kowloon, Hong Kong, Peoples Republic of China. tlims00@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21245394

Citation

Leung, Christopher Kai-shun, et al. "Long-term in Vivo Imaging and Measurement of Dendritic Shrinkage of Retinal Ganglion Cells." Investigative Ophthalmology & Visual Science, vol. 52, no. 3, 2011, pp. 1539-47.
Leung CK, Weinreb RN, Li ZW, et al. Long-term in vivo imaging and measurement of dendritic shrinkage of retinal ganglion cells. Invest Ophthalmol Vis Sci. 2011;52(3):1539-47.
Leung, C. K., Weinreb, R. N., Li, Z. W., Liu, S., Lindsey, J. D., Choi, N., Liu, L., Cheung, C. Y., Ye, C., Qiu, K., Chen, L. J., Yung, W. H., Crowston, J. G., Pu, M., So, K. F., Pang, C. P., & Lam, D. S. (2011). Long-term in vivo imaging and measurement of dendritic shrinkage of retinal ganglion cells. Investigative Ophthalmology & Visual Science, 52(3), 1539-47. https://doi.org/10.1167/iovs.10-6012
Leung CK, et al. Long-term in Vivo Imaging and Measurement of Dendritic Shrinkage of Retinal Ganglion Cells. Invest Ophthalmol Vis Sci. 2011 Mar 1;52(3):1539-47. PubMed PMID: 21245394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-term in vivo imaging and measurement of dendritic shrinkage of retinal ganglion cells. AU - Leung,Christopher Kai-shun, AU - Weinreb,Robert N, AU - Li,Zhi Wei, AU - Liu,Shu, AU - Lindsey,James D, AU - Choi,Nathan, AU - Liu,Lan, AU - Cheung,Carol Yim-lui, AU - Ye,Cong, AU - Qiu,Kunliang, AU - Chen,Li Jia, AU - Yung,Wing Ho, AU - Crowston,Jonathan G, AU - Pu,Mingliang, AU - So,Kwok Fai, AU - Pang,Chi Pui, AU - Lam,Dennis Shun Chiu, Y1 - 2011/03/01/ PY - 2011/1/20/entrez PY - 2011/1/20/pubmed PY - 2011/5/21/medline SP - 1539 EP - 47 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 52 IS - 3 N2 - PURPOSE: To monitor and measure dendritic shrinkage of retinal ganglion cells (RGCs) in a strain of transgenic mice (Thy-1 YFP) that expresses yellow fluorescent proteins in neurons under the control of a Thy-1 promoter. METHODS: A total of 125 RGCs from 16 eyes of Thy-1 YFP transgenic mice were serially imaged with a confocal scanning laser ophthalmoscope for 6 months after optic nerve crush. Quantitative analysis of cell body area, axon diameter, dendritic field, number of terminal branches, total dendritic branch length, branching complexity, symmetry, and distance from the optic disc was used to characterize the morphology of RGCs, describe the patterns of axonal and dendritic degeneration, identify the morphologic predictors for cell survival, and estimate the rate of dendritic shrinkage. RESULTS: RGC damage was observed prospectively to begin with progressive dendritic shrinkage, followed by loss of the axon and the cell body. In a small proportion of RGCs, progressive axonal changes including fragmentation, beading, retraction, and bulb formation were also observed. RGCs with a larger dendritic field and a longer total dendritic branch length in general have a better survival probability. The rate of dendritic shrinkage was variable with a slower rate observed in cells having a larger dendritic field, a longer total dendritic branch length, and a greater distance from the optic disc. CONCLUSIONS: Estimating the probability of RGC survival and measuring the rate of dendritic shrinkage could become a new paradigm for investigating neuronal degeneration and evaluating the response of neuroprotective treatment. SN - 1552-5783 UR - https://www.unboundmedicine.com/medline/citation/21245394/Long_term_in_vivo_imaging_and_measurement_of_dendritic_shrinkage_of_retinal_ganglion_cells_ L2 - https://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.10-6012 DB - PRIME DP - Unbound Medicine ER -