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Temporal fluctuation of multidrug resistant salmonella typhi haplotypes in the mekong river delta region of Vietnam.
PLoS Negl Trop Dis. 2011 Jan 04; 5(1):e929.PN

Abstract

BACKGROUND

typhoid fever remains a public health problem in Vietnam, with a significant burden in the Mekong River delta region. Typhoid fever is caused by the bacterial pathogen Salmonella enterica serovar Typhi (S. Typhi), which is frequently multidrug resistant with reduced susceptibility to fluoroquinolone-based drugs, the first choice for the treatment of typhoid fever. We used a GoldenGate (Illumina) assay to type 1,500 single nucleotide polymorphisms (SNPs) and analyse the genetic variation of S. Typhi isolated from 267 typhoid fever patients in the Mekong delta region participating in a randomized trial conducted between 2004 and 2005.

PRINCIPAL FINDINGS

the population of S. Typhi circulating during the study was highly clonal, with 91% of isolates belonging to a single clonal complex of the S. Typhi H58 haplogroup. The patterns of disease were consistent with the presence of an endemic haplotype H58-C and a localised outbreak of S. Typhi haplotype H58-E2 in 2004. H58-E2-associated typhoid fever cases exhibited evidence of significant geo-spatial clustering along the Sông H u branch of the Mekong River. Multidrug resistance was common in the established clone H58-C but not in the outbreak clone H58-E2, however all H58 S. Typhi were nalidixic acid resistant and carried a Ser83Phe amino acid substitution in the gyrA gene.

SIGNIFICANCE

the H58 haplogroup dominates S. Typhi populations in other endemic areas, but the population described here was more homogeneous than previously examined populations, and the dominant clonal complex (H58-C, -E1, -E2) observed in this study has not been detected outside Vietnam. IncHI1 plasmid-bearing S. Typhi H58-C was endemic during the study period whilst H58-E2, which rarely carried the plasmid, was only transient, suggesting a selective advantage for the plasmid. These data add insight into the outbreak dynamics and local molecular epidemiology of S. Typhi in southern Vietnam.

Authors+Show Affiliations

The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom. kholt@unimelb.edu.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Multicenter Study
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21245916

Citation

Holt, Kathryn E., et al. "Temporal Fluctuation of Multidrug Resistant Salmonella Typhi Haplotypes in the Mekong River Delta Region of Vietnam." PLoS Neglected Tropical Diseases, vol. 5, no. 1, 2011, pp. e929.
Holt KE, Dolecek C, Chau TT, et al. Temporal fluctuation of multidrug resistant salmonella typhi haplotypes in the mekong river delta region of Vietnam. PLoS Negl Trop Dis. 2011;5(1):e929.
Holt, K. E., Dolecek, C., Chau, T. T., Duy, P. T., La, T. T., Hoang, N. V., Nga, T. V., Campbell, J. I., Manh, B. H., Vinh Chau, N. V., Hien, T. T., Farrar, J., Dougan, G., & Baker, S. (2011). Temporal fluctuation of multidrug resistant salmonella typhi haplotypes in the mekong river delta region of Vietnam. PLoS Neglected Tropical Diseases, 5(1), e929. https://doi.org/10.1371/journal.pntd.0000929
Holt KE, et al. Temporal Fluctuation of Multidrug Resistant Salmonella Typhi Haplotypes in the Mekong River Delta Region of Vietnam. PLoS Negl Trop Dis. 2011 Jan 4;5(1):e929. PubMed PMID: 21245916.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Temporal fluctuation of multidrug resistant salmonella typhi haplotypes in the mekong river delta region of Vietnam. AU - Holt,Kathryn E, AU - Dolecek,Christiane, AU - Chau,Tran Thuy, AU - Duy,Pham Thanh, AU - La,Tran Thi Phi, AU - Hoang,Nguyen Van Minh, AU - Nga,Tran Vu Thieu, AU - Campbell,James I, AU - Manh,Bui Huu, AU - Vinh Chau,Nguyen Van, AU - Hien,Tran Tinh, AU - Farrar,Jeremy, AU - Dougan,Gordon, AU - Baker,Stephen, Y1 - 2011/01/04/ PY - 2010/07/27/received PY - 2010/11/30/accepted PY - 2011/1/20/entrez PY - 2011/1/20/pubmed PY - 2011/4/22/medline SP - e929 EP - e929 JF - PLoS neglected tropical diseases JO - PLoS Negl Trop Dis VL - 5 IS - 1 N2 - BACKGROUND: typhoid fever remains a public health problem in Vietnam, with a significant burden in the Mekong River delta region. Typhoid fever is caused by the bacterial pathogen Salmonella enterica serovar Typhi (S. Typhi), which is frequently multidrug resistant with reduced susceptibility to fluoroquinolone-based drugs, the first choice for the treatment of typhoid fever. We used a GoldenGate (Illumina) assay to type 1,500 single nucleotide polymorphisms (SNPs) and analyse the genetic variation of S. Typhi isolated from 267 typhoid fever patients in the Mekong delta region participating in a randomized trial conducted between 2004 and 2005. PRINCIPAL FINDINGS: the population of S. Typhi circulating during the study was highly clonal, with 91% of isolates belonging to a single clonal complex of the S. Typhi H58 haplogroup. The patterns of disease were consistent with the presence of an endemic haplotype H58-C and a localised outbreak of S. Typhi haplotype H58-E2 in 2004. H58-E2-associated typhoid fever cases exhibited evidence of significant geo-spatial clustering along the Sông H u branch of the Mekong River. Multidrug resistance was common in the established clone H58-C but not in the outbreak clone H58-E2, however all H58 S. Typhi were nalidixic acid resistant and carried a Ser83Phe amino acid substitution in the gyrA gene. SIGNIFICANCE: the H58 haplogroup dominates S. Typhi populations in other endemic areas, but the population described here was more homogeneous than previously examined populations, and the dominant clonal complex (H58-C, -E1, -E2) observed in this study has not been detected outside Vietnam. IncHI1 plasmid-bearing S. Typhi H58-C was endemic during the study period whilst H58-E2, which rarely carried the plasmid, was only transient, suggesting a selective advantage for the plasmid. These data add insight into the outbreak dynamics and local molecular epidemiology of S. Typhi in southern Vietnam. SN - 1935-2735 UR - https://www.unboundmedicine.com/medline/citation/21245916/Temporal_fluctuation_of_multidrug_resistant_salmonella_typhi_haplotypes_in_the_mekong_river_delta_region_of_Vietnam_ L2 - https://dx.plos.org/10.1371/journal.pntd.0000929 DB - PRIME DP - Unbound Medicine ER -