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In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus.
Virol J. 2011 Jan 19; 8:26.VJ

Abstract

BACKGROUND

Hepatitis C is a major health problem causes liver cirrhosis, hepatocellular carcinoma and death. The current treatment of standard interferon in combination with ribavirin, has limited benefits due to emergence of resistant mutations during long-term treatment, adverse side effects and high cost. Hence, there is a need for the development of more effective, less toxic antiviral agents.

RESULTS

The present study was designed to search anti-HCV plants from different areas of Pakistan. Ten medicinal plants were collected and tested for anti-HCV activity by infecting the liver cells with HCV 3a innoculum. Methanol and chloroform extracts of Solanum nigrum (SN) seeds exhibited 37% and more than 50% inhibition of HCV respectively at non toxic concentration. Moreover, antiviral effect of SN seeds extract was also analyzed against HCV NS3 protease by transfecting HCV NS3 protease plasmid into liver cells. The results demonstrated that chloroform extract of SN decreased the expression or function of HCV NS3 protease in a dose- dependent manner and GAPDH remained constant.

CONCLUSION

These results suggest that SN extract contains potential antiviral agents against HCV and combination of SN extract with interferon will be better option to treat chronic HCV.

Authors+Show Affiliations

Division of Molecular Medicine, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21247464

Citation

Javed, Tariq, et al. "In-vitro Antiviral Activity of Solanum Nigrum Against Hepatitis C Virus." Virology Journal, vol. 8, 2011, p. 26.
Javed T, Ashfaq UA, Riaz S, et al. In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus. Virol J. 2011;8:26.
Javed, T., Ashfaq, U. A., Riaz, S., Rehman, S., & Riazuddin, S. (2011). In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus. Virology Journal, 8, 26. https://doi.org/10.1186/1743-422X-8-26
Javed T, et al. In-vitro Antiviral Activity of Solanum Nigrum Against Hepatitis C Virus. Virol J. 2011 Jan 19;8:26. PubMed PMID: 21247464.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus. AU - Javed,Tariq, AU - Ashfaq,Usman Ali, AU - Riaz,Sana, AU - Rehman,Sidra, AU - Riazuddin,Sheikh, Y1 - 2011/01/19/ PY - 2010/12/13/received PY - 2011/01/19/accepted PY - 2011/1/21/entrez PY - 2011/1/21/pubmed PY - 2011/5/4/medline SP - 26 EP - 26 JF - Virology journal JO - Virol. J. VL - 8 N2 - BACKGROUND: Hepatitis C is a major health problem causes liver cirrhosis, hepatocellular carcinoma and death. The current treatment of standard interferon in combination with ribavirin, has limited benefits due to emergence of resistant mutations during long-term treatment, adverse side effects and high cost. Hence, there is a need for the development of more effective, less toxic antiviral agents. RESULTS: The present study was designed to search anti-HCV plants from different areas of Pakistan. Ten medicinal plants were collected and tested for anti-HCV activity by infecting the liver cells with HCV 3a innoculum. Methanol and chloroform extracts of Solanum nigrum (SN) seeds exhibited 37% and more than 50% inhibition of HCV respectively at non toxic concentration. Moreover, antiviral effect of SN seeds extract was also analyzed against HCV NS3 protease by transfecting HCV NS3 protease plasmid into liver cells. The results demonstrated that chloroform extract of SN decreased the expression or function of HCV NS3 protease in a dose- dependent manner and GAPDH remained constant. CONCLUSION: These results suggest that SN extract contains potential antiviral agents against HCV and combination of SN extract with interferon will be better option to treat chronic HCV. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/21247464/In_vitro_antiviral_activity_of_Solanum_nigrum_against_Hepatitis_C_Virus_ L2 - https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-8-26 DB - PRIME DP - Unbound Medicine ER -