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Prospective evaluation of nonstructural 1 enzyme-linked immunosorbent assay and rapid immunochromatographic tests to detect dengue virus in patients with acute febrile illness.
Diagn Microbiol Infect Dis. 2011 Feb; 69(2):172-8.DM

Abstract

We prospectively evaluated the Bio-Rad nonstructural 1 (NS1) enzyme-linked immunosorbent assay (ELISA) and lateral flow immunochromatographic assay (LFIA) in comparison to an in-place reverse transcription-polymerase chain reaction for dengue diagnosis. Among 537 consecutive samples from patients with acute febrile disease, 264 (49.2%) tested positive in reverse transcription-polymerase chain reaction (RT-PCR), 156 (29.1%) in NS1-antigen (Ag) ELISA, and 125 (23.3%) in NS1-Ag LFIA. Compared to the RT-PCR status, the specificity was 100% for the NS1-Ag ELISA and LFIA, but their respective sensitivities were 61.2% [95% confidence interval (CI), 55.2-67.2] and 49.4% (95% CI, 43.2-55.6), with nadirs of 37.9% and 24.1% on day 6 of illness. The NS1-Ag ELISA and LFIA were positive, respectively, for 48.0% and 40.7% of the secondary infections versus 85.0% and 66.7% of the primary infections. For patients <5 years old, NS1-Ag ELISA and LFIA reached respective sensitivities of 100% and 90.5%. Reports of results of dengue NS1-Ag assays should specify that negativity does not preclude DENV infection, and require further investigations in the case of severe disease.

Authors+Show Affiliations

Laboratoire de Virologie-Immunologie, EA 4537, Centre Hospitalier Universitaire de Fort-de-France, Université des Antilles et de la Guyane, 97200 Fort-de-France, Martinique.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

21251561

Citation

Najioullah, Fatiha, et al. "Prospective Evaluation of Nonstructural 1 Enzyme-linked Immunosorbent Assay and Rapid Immunochromatographic Tests to Detect Dengue Virus in Patients With Acute Febrile Illness." Diagnostic Microbiology and Infectious Disease, vol. 69, no. 2, 2011, pp. 172-8.
Najioullah F, Combet E, Paturel L, et al. Prospective evaluation of nonstructural 1 enzyme-linked immunosorbent assay and rapid immunochromatographic tests to detect dengue virus in patients with acute febrile illness. Diagn Microbiol Infect Dis. 2011;69(2):172-8.
Najioullah, F., Combet, E., Paturel, L., Martial, J., Koulmann, L., Thomas, L., Hatchuel, Y., Cabié, A., & Cesaire, R. (2011). Prospective evaluation of nonstructural 1 enzyme-linked immunosorbent assay and rapid immunochromatographic tests to detect dengue virus in patients with acute febrile illness. Diagnostic Microbiology and Infectious Disease, 69(2), 172-8. https://doi.org/10.1016/j.diagmicrobio.2010.09.021
Najioullah F, et al. Prospective Evaluation of Nonstructural 1 Enzyme-linked Immunosorbent Assay and Rapid Immunochromatographic Tests to Detect Dengue Virus in Patients With Acute Febrile Illness. Diagn Microbiol Infect Dis. 2011;69(2):172-8. PubMed PMID: 21251561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prospective evaluation of nonstructural 1 enzyme-linked immunosorbent assay and rapid immunochromatographic tests to detect dengue virus in patients with acute febrile illness. AU - Najioullah,Fatiha, AU - Combet,Emilie, AU - Paturel,Laure, AU - Martial,Jenny, AU - Koulmann,Laurence, AU - Thomas,Laurent, AU - Hatchuel,Yves, AU - Cabié,André, AU - Cesaire,Raymond, PY - 2010/06/19/received PY - 2010/09/28/revised PY - 2010/09/28/accepted PY - 2011/1/22/entrez PY - 2011/1/22/pubmed PY - 2011/11/8/medline SP - 172 EP - 8 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 69 IS - 2 N2 - We prospectively evaluated the Bio-Rad nonstructural 1 (NS1) enzyme-linked immunosorbent assay (ELISA) and lateral flow immunochromatographic assay (LFIA) in comparison to an in-place reverse transcription-polymerase chain reaction for dengue diagnosis. Among 537 consecutive samples from patients with acute febrile disease, 264 (49.2%) tested positive in reverse transcription-polymerase chain reaction (RT-PCR), 156 (29.1%) in NS1-antigen (Ag) ELISA, and 125 (23.3%) in NS1-Ag LFIA. Compared to the RT-PCR status, the specificity was 100% for the NS1-Ag ELISA and LFIA, but their respective sensitivities were 61.2% [95% confidence interval (CI), 55.2-67.2] and 49.4% (95% CI, 43.2-55.6), with nadirs of 37.9% and 24.1% on day 6 of illness. The NS1-Ag ELISA and LFIA were positive, respectively, for 48.0% and 40.7% of the secondary infections versus 85.0% and 66.7% of the primary infections. For patients <5 years old, NS1-Ag ELISA and LFIA reached respective sensitivities of 100% and 90.5%. Reports of results of dengue NS1-Ag assays should specify that negativity does not preclude DENV infection, and require further investigations in the case of severe disease. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/21251561/Prospective_evaluation_of_nonstructural_1_enzyme_linked_immunosorbent_assay_and_rapid_immunochromatographic_tests_to_detect_dengue_virus_in_patients_with_acute_febrile_illness_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(10)00435-9 DB - PRIME DP - Unbound Medicine ER -