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Design of an imprinted clean-up method for mycophenolic acid in maize.
J Chromatogr A. 2011 Feb 25; 1218(8):1122-30.JC

Abstract

In the present work, the development of imprinted polymers selective towards mycophenolic acid and their application in food analysis are reported for the first time. To synthesize the molecularly imprinted polymer (MIP) 4-vinylpyridine and ethyleneglycol dimethacrylate were applied as functional monomer and cross-linker, respectively. Besides the toxin itself, the implementation of structural analogues as templates was evaluated. A molecularly imprinted solid-phase extraction (MISPE) procedure was designed for the selective clean-up of maize extracts. Binding experiments and Scatchard analysis indicated the presence of specific binding sites in the imprinted polymers. The imprinting effect varied along with the selected template. The dissociation constant (K(D)) of the higher affinity binding sites ranged from 0.8 μmol/l to 15.6 μmol/l, while the K(D) of the lower affinity binding sites was in the range of 138.5-519.3 μmol/l. The performance of the MIPs throughout the clean-up of spiked maize sample extracts was evaluated and compared with the results obtained when applying a non-imprinted polymer. Depending on the polymers and the spiked concentration, recoveries after MISPE and non-imprinted solid-phase extraction varied respectively from 49% to 84% and from 28% to 31%. The imprinted polymers were superior regarding matrix effect, limit of detection (LOD) and limit of quantification (LOQ). LOD ranged from 0.17 μg/kg to 0.25 μg/kg and LOQ varied from 0.57 μg/kg to 0.82 μg/kg. Analysis of 15 maize samples by liquid chromatography tandem mass spectrometry revealed that the MIPs could be excellent sorbents for clean-up of contaminated food samples.

Authors+Show Affiliations

Ghent University, Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Harelbekestraat 72, 9000 Ghent, Belgium. david.desmet@ugent.beNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21251660

Citation

De Smet, David, et al. "Design of an Imprinted Clean-up Method for Mycophenolic Acid in Maize." Journal of Chromatography. A, vol. 1218, no. 8, 2011, pp. 1122-30.
De Smet D, Kodeck V, Dubruel P, et al. Design of an imprinted clean-up method for mycophenolic acid in maize. J Chromatogr A. 2011;1218(8):1122-30.
De Smet, D., Kodeck, V., Dubruel, P., Van Peteghem, C., Schacht, E., & De Saeger, S. (2011). Design of an imprinted clean-up method for mycophenolic acid in maize. Journal of Chromatography. A, 1218(8), 1122-30. https://doi.org/10.1016/j.chroma.2010.12.085
De Smet D, et al. Design of an Imprinted Clean-up Method for Mycophenolic Acid in Maize. J Chromatogr A. 2011 Feb 25;1218(8):1122-30. PubMed PMID: 21251660.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Design of an imprinted clean-up method for mycophenolic acid in maize. AU - De Smet,David, AU - Kodeck,Valérie, AU - Dubruel,Peter, AU - Van Peteghem,Carlos, AU - Schacht,Etienne, AU - De Saeger,Sarah, Y1 - 2010/12/28/ PY - 2010/09/03/received PY - 2010/12/17/revised PY - 2010/12/20/accepted PY - 2011/1/22/entrez PY - 2011/1/22/pubmed PY - 2011/5/11/medline SP - 1122 EP - 30 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1218 IS - 8 N2 - In the present work, the development of imprinted polymers selective towards mycophenolic acid and their application in food analysis are reported for the first time. To synthesize the molecularly imprinted polymer (MIP) 4-vinylpyridine and ethyleneglycol dimethacrylate were applied as functional monomer and cross-linker, respectively. Besides the toxin itself, the implementation of structural analogues as templates was evaluated. A molecularly imprinted solid-phase extraction (MISPE) procedure was designed for the selective clean-up of maize extracts. Binding experiments and Scatchard analysis indicated the presence of specific binding sites in the imprinted polymers. The imprinting effect varied along with the selected template. The dissociation constant (K(D)) of the higher affinity binding sites ranged from 0.8 μmol/l to 15.6 μmol/l, while the K(D) of the lower affinity binding sites was in the range of 138.5-519.3 μmol/l. The performance of the MIPs throughout the clean-up of spiked maize sample extracts was evaluated and compared with the results obtained when applying a non-imprinted polymer. Depending on the polymers and the spiked concentration, recoveries after MISPE and non-imprinted solid-phase extraction varied respectively from 49% to 84% and from 28% to 31%. The imprinted polymers were superior regarding matrix effect, limit of detection (LOD) and limit of quantification (LOQ). LOD ranged from 0.17 μg/kg to 0.25 μg/kg and LOQ varied from 0.57 μg/kg to 0.82 μg/kg. Analysis of 15 maize samples by liquid chromatography tandem mass spectrometry revealed that the MIPs could be excellent sorbents for clean-up of contaminated food samples. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/21251660/Design_of_an_imprinted_clean_up_method_for_mycophenolic_acid_in_maize_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(10)01796-6 DB - PRIME DP - Unbound Medicine ER -