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Sensitivity enhancement of fluorescence detection in CE by coupling and conducting excitation light with tapered optical fiber.
Electrophoresis. 2011 Jan; 32(2):268-74.E

Abstract

This paper reports the enhancement of sensitivity of detection for in-column fiber optic-induced fluorescence detection system in CE by tapered optical fiber (TOF). Two types of optical fiber, TOF and conventional cylindrical optical fiber (COF), were employed to construct the CE (TOF-CE and COF-CE) and were compared for sensitivity to riboflavin (RF). The fluorescence intensities from a RF sample with excitation light sources and fibers at various coupling angles were investigated. The fluorescence signal from TOF-CE was ca. ten times that of COF-CE. In addition, the detection performance of four excitation light source-fiber configurations including Laser-TOF, Laser-COF, LED-TOF, and LED-COF were compared. The LODs for RF were 0.21, 0.82, 0.80, and 7.5 nM, respectively, for the four excitation light source-fiber configurations. The results demonstrate that the sensitivity obtained by LED-TOF is close to that of Laser-COF. Both Laser-TOF and LED-TOF can greatly improve the sensitivity of detection in CE. TOF has the major attribute of collecting and focusing the excitation light intensity. Thus, the sensitivity obtained by LED-TOF without focusing lens is just same as that of LED-COF with a focusing lens. This demonstrates that the CE system can be further simplified by eliminating the focusing lens for excitation light. LED-TOF-CE and LED-COF-CE system were applied to the separation and determination of RF in real sample (green tea), respectively. The tapered fiber optic-induced fluorescence detection system in CE is an ideal tool for trace analysis.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Yang X
College of Chemistry and College of Chemical Engineering, Sichuan University, Chengdu, P. R. China.
Huo F
No affiliation info available
Yuan H
No affiliation info available
Zhang B
No affiliation info available
Xiao D
No affiliation info available
Choi MM
No affiliation info available

MeSH

Electrophoresis, CapillaryOptical FibersRiboflavinSensitivity and SpecificitySpectrometry, Fluorescence

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21254125

Citation

Yang, Xiupei, et al. "Sensitivity Enhancement of Fluorescence Detection in CE By Coupling and Conducting Excitation Light With Tapered Optical Fiber." Electrophoresis, vol. 32, no. 2, 2011, pp. 268-74.
Yang X, Huo F, Yuan H, et al. Sensitivity enhancement of fluorescence detection in CE by coupling and conducting excitation light with tapered optical fiber. Electrophoresis. 2011;32(2):268-74.
Yang, X., Huo, F., Yuan, H., Zhang, B., Xiao, D., & Choi, M. M. (2011). Sensitivity enhancement of fluorescence detection in CE by coupling and conducting excitation light with tapered optical fiber. Electrophoresis, 32(2), 268-74. https://doi.org/10.1002/elps.201000340
Yang X, et al. Sensitivity Enhancement of Fluorescence Detection in CE By Coupling and Conducting Excitation Light With Tapered Optical Fiber. Electrophoresis. 2011;32(2):268-74. PubMed PMID: 21254125.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitivity enhancement of fluorescence detection in CE by coupling and conducting excitation light with tapered optical fiber. AU - Yang,Xiupei, AU - Huo,Feng, AU - Yuan,Hongyan, AU - Zhang,Bo, AU - Xiao,Dan, AU - Choi,Martin M F, Y1 - 2010/12/14/ PY - 2010/03/23/received PY - 2010/10/18/revised PY - 2010/10/18/accepted PY - 2011/1/22/entrez PY - 2011/1/22/pubmed PY - 2011/6/22/medline SP - 268 EP - 74 JF - Electrophoresis JO - Electrophoresis VL - 32 IS - 2 N2 - This paper reports the enhancement of sensitivity of detection for in-column fiber optic-induced fluorescence detection system in CE by tapered optical fiber (TOF). Two types of optical fiber, TOF and conventional cylindrical optical fiber (COF), were employed to construct the CE (TOF-CE and COF-CE) and were compared for sensitivity to riboflavin (RF). The fluorescence intensities from a RF sample with excitation light sources and fibers at various coupling angles were investigated. The fluorescence signal from TOF-CE was ca. ten times that of COF-CE. In addition, the detection performance of four excitation light source-fiber configurations including Laser-TOF, Laser-COF, LED-TOF, and LED-COF were compared. The LODs for RF were 0.21, 0.82, 0.80, and 7.5 nM, respectively, for the four excitation light source-fiber configurations. The results demonstrate that the sensitivity obtained by LED-TOF is close to that of Laser-COF. Both Laser-TOF and LED-TOF can greatly improve the sensitivity of detection in CE. TOF has the major attribute of collecting and focusing the excitation light intensity. Thus, the sensitivity obtained by LED-TOF without focusing lens is just same as that of LED-COF with a focusing lens. This demonstrates that the CE system can be further simplified by eliminating the focusing lens for excitation light. LED-TOF-CE and LED-COF-CE system were applied to the separation and determination of RF in real sample (green tea), respectively. The tapered fiber optic-induced fluorescence detection system in CE is an ideal tool for trace analysis. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/21254125/Sensitivity_enhancement_of_fluorescence_detection_in_CE_by_coupling_and_conducting_excitation_light_with_tapered_optical_fiber_ L2 - https://doi.org/10.1002/elps.201000340 DB - PRIME DP - Unbound Medicine ER -